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عضویت
فهرست مطالب نویسنده:

mohammad hassan bazafkan

  • سهند جرفی *، نعمت الله جعفرزاده حقیقی فرد، مهدی احمدی، افشین تکدستان، محمدحسن بازافکن، سیده مریم موسوی، سمانه میرعالی
    زمینه
    PAHs محصول احتراق ناقص سوخت های فسیلی بوده و به دلیل ماهیت نشر، آلاینده خاک و سواحل به شمار می روند. این ترکیبات در زمره آلاینده های دارای اولویت، سرطان زا و جهش زای قطعی به شمار می روند. دشواری اصلی پاکسازی مناطق آلوده به PAHs، ماهیت به شدت آبگریز این آلاینده ها و جذب شدید آنها به بافت خاک می باشد. هدف اصلی این پژوهش تعیین بازده حذف فنانترن از خاک و سواحل آلوده با استفاده بیوسورفکتانت تولید شده از گونه باکتریایی جداسازی شده از خلیج فارس بود.
    مواد و روش ها
    با غربالگری اولیه، یک گونه باسیلوس sp با قابلیت تولید سورفکتین در آزمایشگاه جداسازی و خالص سازی گردید. یک کنسرسیوم باکتریایی مخلوط متشکل از سه گونه باکتریایی با قابلیت متابولیسم فنانترن از سواحل آلوده خارک جداسازی و به عنوان بذر میکروبی استفاده شد. نمونه های خاک های دارای آلودکی مصنوعی با غلظت اولیه میلی گرم بر کیلوگرم 100 و دارای آلودگی طبیعی طی 9 هفته متوالی مورد اصلاح زیستی قرار گرفتند.
    یافته ها
    بازده حذف فنانترن در نمونه های حاوی بیوسورفکتانت و دارای آلودگی مصنوعی و طبیعی به ترتیب 82 درصد و 39 درصد بود. بازده حذف در نمونه های دارای آلودگی مصنوعی و فاقد بیوسورفکتانت 11 درصد بود.
    نتیجه گیری
    فرایند اصلاح زیستی با استفاده از بیوسورفکتانت های میکروبی گزینه ای کارامد، دوست دار محیط زیست و عملیاتی برای اصلاح سواحل و خاک های آلوده به هیدروکربن های نفتی به شمار می رود.
    کلید واژگان: آلودگی سواحل, PAHs, بیوسورفکتانت, اصلاح زیستی
    Sahand Jorfi *, Neamatollah Jafarzadeh Haghighifard, Mehdi Ahmadi, Afshin Takdastan, Mohammadhassan Bazafkan, Seyadeh Maryam Mousavi, Samaneh Mirali
    Background
    PAHs was producted from incomplete combustion of fossil fuels and due to nature of publishing, it was categorized as the soil and beaches pollutant. These compounds are considered in pollutants which have priority, carcinogenic and certain mutagenic. The main difficulty of clearing contaminated areas to PAHs is the nature of highly water repellent of these pollutants and a strong attraction to the soil texture. The main objective of this current study was to determine the efficiency of phenanthrene removal from contaminated soil and beaches by using biosurfactant produced by a bacterium isolated from Persian Gulf.
    Materials and Methods
    with primary screening, a Bacillus sp strain with surfactin production capability was isolated and purified in laboratory. A mixed bacterial consortium isolated which was consists of three bacterial species with of capable of metabolism of phenanthrene from Khark contaminated beaches and was used as a microbial seed. The synthetic soil samples with initial phenanthrene concentration of 100 mg/kg and also natural contaminated samples were subjected to bioremediation during 9 weeks.
    Results
    The phenanthrene removal efficiency in the samples containing biosurfactants and with artificial and natural pollution were 82% and 39% respectively. The removal efficiency for samples without biosurfactant was 11%.
    Conclusion
    The bioremediation process is considered an efficient, eco-friendly and operational for remediation of beache and soil polluted by petroleum hydrocarbons by using bacterial biosurfactant.
    Keywords: beches polluted, PAHs, Biosurfactan, Bioremediation
  • Mohammad Hassan Bazafkan, Ameneh Hardani *, Mohammad Reza Afzal Zadeh, Ashraf Amir Zargar, Najme Moradi, Nasrin Jalali
    Background
    For many centuries, the human has made a lot of efforts to determine sex ratios in different species and has proposed various theories. In this regard, the effect of several plants on sex ratio (female to male) has been examined, but an empirical research about the effect of aqueous extracts from the leaves of celery on the sex ratio has not been carried out yet..
    Objectives
    Therefore, this study aimed to evaluate the effects of celery extract on the delivery rate, sex ratio, and the number of newborns in female rats..Patients and
    Methods
    A total of 45 adult Wistar rats (15 Males and 30 Females) were prepared. The rats were divided into 3 groups; a control group and the 2 other experimental groups received celery extracts at doses of 100 and 200 mg/kg of their body weight. The extract was given orally to the female rats by gavage for 15 consecutive days. For mating, each male rat was placed with two female rats in separate cages for five days. After delivery, the percentage of rats that gave birth, as well as the number and sex ratio of the newborns were determined. The results were analyzed using SPSS version, 1-way analysis of variance (ANOVA) test, Tukey test, and post hoc LSD..
    Results
    The results showed that aqueous extracts of celery have significantly increased the mean percentage of delivery rate (extract, and control were 100 ± 60 vs. 60 ± 0.16, respectively), the average number of newborns (extract, and control were 7.8 ± 1.2 vs. 3.5 ± 1.2, respectively) and the mean sex ratio (male to female ratio) of newborns (extract and control were 1.26 ± 0.37 vs. 0.81 ± 0.13, respectively) (P ≤ 0. 05)..
    Conclusions
    The extract contains substances that are effective on the delivery rate, sex ratio, and the number of newborns in the female rats. It may affect through the ionic changes in the rats, by modifying the activity of follicle-stimulating and testosterone hormones, and/or changing the rate of fertilizing ability of sperms containing Y chromosome. This phenomenon may also be due to lowering serum fats, acidity levels, and accordingly reducing the acidity of the uterus secretions.
    Keywords: Apium Graveolens, Sex Ratio, Female Rat
  • Mohammad Hassan Bazafkan, Ameneh Hardani *, Mohammad Reza Afzal Zadeh, Ashraf Amir Zargar, Mahmoud Orazi Zadeh, Ali Asghar Hemmati, Esrafil Mansori, Majid Asadi-Samani, Maryam Ghasemiboroon, Vesam Kooti
    Background
    In Iranian traditional medicine, Punica granatum, cabbage, and plantago have been used to prompt wound healing. The effects of cabbage, Punica granatum, and Plantago in wound healing have been investigated to some extent, but no experimental study has been performed to assess the impact of an ointment containing all these three substances on wound healing..
    Objectives
    The aim of the present study was to evaluate the effect of an ointment prepared from a mixture of cabbage, Punica granatum, and plantago on local wound healing in Wistar rats..
    Materials And Methods
    This experimental study was performed on 21 male Wistar rats. Firstly, a full thickness wound was created on rats flank, near the spine by a bistoury blade and forceps. Then they were randomly divided into three groups of seven rats as the control group, a positive control group (the treatment group with phenytoin), and the third one received a treatment with studied ointment. The wounds were evaluated on days three, six, nine, and 12. Results were compared by SPSS software using ANOVA test..
    Results
    The average percentage of wound healing in different groups on days 3, 6, 9 and 12 were as follows; 21.3%, 37.87%, 67.39% and 77.17% in control group; 24.98%, 34.21%, 70.74% and 88.55% in positive control group; and 32.35%, 61.27%, 94.53%, and 99.91%, in case group. A significant difference was observed between the control and case groups (P < 0.05)..
    Keywords: Wound Healing, Punica granatum, Brassica oleracea, Plantago major L
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