فهرست مطالب mohammad hossein nazaran

The treatment of prostate cancer patients usually starts with androgen ablation and followed by chemotherapy; however, in some cases the tumor develops resistant phenotype. Combination therapy is currently regarded as a cornerstone in cancer therapy to overcome the drug resistance. Herein, we investigated the combinatory effect of Docetaxel and Trastuzumab with a novel nanomedicine, BCc1. Also, we knocked down the expression of Heat shock factor-1, HSF1, in resistant Prostate Cancer cell line 3, PC3, using RNA interference, RNAi, to sensitize the cancer cells to the drug treatment. We observed down-regulation of Erb-B2 Receptor Tyrosine Kinase 3, ERBB3, B-Cell Leukemia/Lymphoma 2, BCL2, and Heat Shock Protein 90, HSP90, in HSF1 knockdown PC3 cells.  Knockdown of HSF1 made PC3 cells more susceptible to Docetaxel treatment. Additionally, BCc1 nanomedicine was tested on prostate cancer cell line PC3 for the first time. It resulted in reduced metabolic activity in these cells.  We propose that a combination of the gene therapy and the chemotherapy gives more favorable results in the treatment of refractory prostate cancer.

The aim of this study was to investigate the effect of mineral supplementation and chelates of trace elements on production, milk composition and blood parameters of lactating Holstein cows. Lactating Holstein cows (n=24) were randomly assigned to one of the following four treatments; 1) the basic diet include inorganic trace minerals (C), 2) 55 mg of iron, 125 mg of copper, 360 mg of zinc, 3.5 mg of chromium, 200 mg of manganese, 2 mg of selenium and 12 mg of cobalt with daily feeding of 7 g of Bonzaplex7 chelated mineral supplement (B), 3) the same amount of zinc, manganese, copper and cobalt was supplied with 7 gr of organic trace mineral supplement Availa4 (A), 4) feeding 7 gr of Availa 4 plus 3.5 gr of Availa-Cr, 2 gr of Availa-Se, 1gr of Availa-Fe (A+3). This study lasted 35 days. Daily feed intake and milk production (3X) were recorded. In spite of more numerical increase in milk yield in treatment B, no effect was observed on the production of milk, milk composition, dry matter intake and rumen metabolites. Plasma glucose concentration in treatments tended to decrease (P=0.07). Plasma triglyceride concentration decreased significantly in treatments (A), (B), (A+3) compared to group (C), present results could be related to increase bioavailability of trace minerals in chelated form specially chromium and copper in present study.