mohammad jahanbakhshi

In order to investigate the effect of foliar application of ascorbic acid on the activity of antioxidant enzymes in mung bean (Vigna radiate L.) under water-deficit stress, a field trial was performed in a split-plot design arranged in completely randomized block design with three replications in the Education and Research Center of Natural Resources Safiabad, Dezful during the two cultivation years (2017 and 2018). Four levels of water-deficit stress treatments were considered in the main plots (25, 50, 75, and 100% of plant water requirement) and combined treatment of foliar application of ascorbic acid in three levels (control, 10, and 20 mM) and genotype including Omidbakhsh VC6173 and Partow cultivars as subplots. The results of mean comparison showed that the highest levels of superoxide dismutase and ascorbate peroxidase were observed in water-deficit stress of 25% water requirement and no foliar application of ascorbic acid. The highest activity of catalase enzyme was observed under water-deficit stress of 50% water requirement and no foliar application of ascorbic acid. Also, the highest activity of guaiacol peroxidase enzyme was observed in Omidbakhsh VC6173 cultivar under water-deficit stress of 25% water requirement and no foliar application of ascorbic acid. The highest glutathione peroxidase activity was observed in VC6173 cultivar under water-deficit stress of 75% water requirement and 10 mM foliar application of ascorbic acid. Results showed that dehydration stress significantly increased the activity of antioxidant enzymes while foliar application of ascorbic acid due to its antioxidant properties reduced the effects of stress and thus reducing the activity of antioxidant enzymes

The anticonvulsant effect of cannabinoid compound has been shown in various models of seizure. On the other hand, there are controversial findings about the role of large conductance calcium-activated potassium (BK) channels in the pathogenesis of epilepsy. In this study, the effect of arachidonyl-2′-chloroethylamide (ACEA), a CB1 receptor agonist, and a BK channel antagonist, paxilline, either alone or in combination was investigated. Both pentylenetetrazole (PTZ) and maximal electroshock (MES) acute models of seizure were used to evaluate the protective effects of drugs. Mice were randomly selected in different groups: (i) control group; (ii) groups received different doses of either paxilline or ACEA; and (iii) groups received combinations of ACEA and paxillin at different doses. In MES model, prevention of hindlimb tonic extension (HLTE) was considered as protective effect. In PTZ model, the required dose of PTZ (mg/kg) to induce tonic-clonic seizure with loss of righting reflex was considered as seizure threshold. In PTZ model, while administration of ACEA per se (5 and 10 mg/kg) caused protective effect against seizure, however, co-administration of ACEA and ineffective doses of paxilline attenuated the antiseizure effects of paxilline. In MES model, while pretreatment by ACEA showed protective effects against seizure, however, co-administration of paxilline and ACEA caused an antagonistic interaction for their antiseizure properties. Our results showed a protective effect of ACEA in both PTZ and MES acute models of seizure. This effect was attenuated by co-administration with paxilline, suggesting the involvement of BK channels in antiseizure activity of ACEA.