فهرست مطالب mohsen ashrafi

 Attention Deficit Hyperactivity Disorder (ADHD) in adulthood is associated with significant impairment in occupational, academic, and social functioning. The aim of this study is to survey the frequency of ADHD in adults referred to psychiatric clinics.

 The present cross-sectional descriptive study includes 300 patients referred to psychiatric clinics affiliated to Babol University of Medical Sciences with an age range of 18-45 years who were selected and included in the study. It is used the Adults Attention Deficit Hyperactivity Disorder self-report scale (ASRS V1.1) to diagnose Adult ADHD in these individuals. Logistic regression and P-Paired test were used to analyze the data.

 The mean age of the subjects was 30.21 ± 7 7.94. Of these, 181 (60.3%) were men and 119 (39.7%) were women. The overall prevalence of Adult ADHD in the study samples was 39.3%. In the logistic regression analysis of crude and adjusted data of study variables, no significant relationship was seen between Adult ADHD and age, education, employment status and marital status (P ≥ 0.05), but a significant relationship between Adult ADHD and consumption of Cigarettes, alcohol and drugs were observed (P ≤ 0.05).

 The findings of the present study show a relatively high prevalence of Adult ADHD among people with a history of psychiatric disorder, who are more likely to be exposed to smoking, alcohol and drug abuse.

Candida albicans is a common yeast in opportunistic fungal diseases world wide, which is frequently colonized on the surface of the skin and mucous membrane. The aim of this study was to investigate the effect of all nanocomposite copper-silver-chitosan on the mentioned yeast. To synthesis of the nanocomposite, first the chitosan was dissolved in water using ultrasonic device, the binding of chitosan and glutaraldehyde was determined by FT-IR technique and the size and morphology of the synthesized nanocomposite were determined by SEM microscopy. In the animal study section, 43 male Wistar rats weighing 200-250 gin 5 groups of 7 were examined. The immune system of mice was weakened by 30 mg/kg cyclophosphamide injection through peritoneal injection and was given candidiasis by oral inoculation of candida suspension. After treatment with the nanocomposite and nystatin, it was found that the number of inflammatory cells in other groups was higher than the group treated with nystatin. Also, the number of cells in nanocomposite coper- silver- chitosan- treated group was much lower than in the other group of mice with weakened immune system and untreated candidiasis. The findings of the present study showed that the therapeutic effect of the nanocomposite against candidiasis was relatively high and had a positive effect on reducing inflammation. Although the anti-yeast effect of copper and chitosan nanoparticles have not been proven separately, but based on the current results, it was found that their combination has a slight synergistic effect and to some extent, slightly effective in their antifungal properties.

Drought as the most important abiotic stress has deleterious effects on plants. Developing drought tolerant varieties can help produce plants in a sustainable way. This study was conducted to identify drought tolerant and drought sensitive thyme species including Thymus vulgaris, T. vulgaris (origin: Spain), T. carmanicus, T. daenensis and T. kotschyanus and to study the mechanism used by them to cope with drought stress. For this purpose, relative water content, water use efficiency, soil water depilation rate, root:shoot ratio, drought resistance index and a new criterion "FC ceased growth" were used. T. carmanicus and T. daenensis had the lowest and the highest reduction on relative water content, respectively. In terms of water use efficiency and soil water depletion curve, the highest and the lowest values were detected for T. daenensis and T. carmanicus, respectively. The most and the least root:shoot ratios were recorded for T. daenensis and T. vulgaris (origin: Spain), respectively. Analyses by drought resistance index and PCA revealed that T. carmanicus is drought susceptible, T. kotschyanus and T. vulgaris are semi-drought susceptible, and T. daenensis and T. vulgaris (origin: Spain) are semi-drought tolerant species. FC ceased growth analysis showed that T. carmanicus stopped its growth at higher FC, while T. kotschyanus stopped it at lower FC. Therefore, based on this criterion and considering the sustainability of growth under drought condition, T. carmanicus and T. kotschyanus are the least and the most drought tolerant Thymus species.

The white button mushroom does not produce remarkable yield in the third flash. Nutritional deficiency and the inability of this mushroom to efficient use of compost are mentioned as its reasons. Basically, compost includes two major food components, lignocellulose and microbial biomass. But this microbial biomass provides just 10% of button mushroom food needs. According to research studies, differentenzymes in both white button mushroom and oyster mushroom are responsible for decomposition of lignin compounds in compost media, from begin of mycelium grows to the end of fruiting. Lacasse, manganese peroxidase, lignin peroxidase, glyoxal oxidase enzymes contribute to degradation of lignin compounds in degradation mushroom has proven by researchers however itis dependent on mushroom types. Manganese peroxidase enzyme (EC. 1.11.1.13) is an extracellular parser lignin enzyme that has a central peroxidase core. Manganese peroxidase enzyme oxidizesMn2 to Mn3 and then Mn3 oxidizes phenolic structure to fonoxile radical. Produced Mn3 is very active and makes complex by chelating organic acids that is produced by mushrooms such as oxalate or malate. Mn3 ions become stable by helping of these chelates and it can penetrate through materials such as wood. On the other hand, in recent years, plant biotechnology provides new solutions for old problems such as use of microorganisms, particularly using bacteria for gene transfer and improvement of superlatives. For a sample of this method, Agrobacterium-mediated transformation system can be noted. In addition, the use of suitable promoters for heterologous genes expression in suitable hosts is an important strategy in functional biotechnology that has been raised in edible mushroom genetic engineering. The lack of efficient and sufficient use of compost, low power of white button mushroom in competition with other rivals, lack of yield per area unit due to production costs, pests and diseases, low flexibility and adaptability with environmental conditions changes are some of the problems that the mushroom reformers are faced. Unlike the great efforts made by researchers, conventional breeding techniques to produce the A. bisporus mushroom only have been led to produce a few new races. Therefore, todays some problems associated with traditional methods of breeding of edible mushrooms, including the need to provide races that have desired characteristics, the traditional method performance tests and low chances of success in the transfer of important agronomic characteristics such as functionality and disease resistance. So, they almost have been replaced with new biotechnology methods. Anexample of this method is to manipulateproperties transformation for the particular purpose. Modification of both expression or type of lignin degrading enzyme are possible solutions to deal with this problem, but these are not applicable or are difficult to be done with traditional breeding programs. In recent years, gene transformation mediated with Agrobacterium routinely is used for gene transformation to mushrooms and is proposed as a method for removing limitations of white button mushroom breeding.
In this research, the oyster mushroom strain Florida was used as the source of manganese peroxidase (mnp) gene and white button mushroom strain 737 gill and cap tissue were used as transformation host. Agrobacterium strain LBA4404 harbors p133H88-FM plasmid thatcontainsmnp gene of oyster mushroom and also hph gene under control of gpdII promoter of the button white mushroom strain IM008 was used as a transformer. Selection medium containing 30 mg/ml Hygromycin B and was used for selecting transformed explants. To confirm transformation, PCR with specific primers of mnp and hph genes was performed on genomic DNA of selected colonies.
Results showed the gill tissue explants, with transformation rate 5%, have a better response to applied transformation method than cap tissue explants, with transformation rate zero percent. As expected, polymerase chain reaction with specific primers ofhph and mnp genes amplified 1049 and 1086 bp fragments and verified the transformation of mycelium's grown on selection medium. It seems that Bacterial strain and also used plasmid were one of the responses for observed low rate transformation which is in accordance with leach and co-workers study. Finally, we could propose that cap tissue is more suitable for further gene transformation of this mushroombecause of high transformation rate of cap tissue.

The similarity of the white button mushroom glycosylation to mammalian cells, caused a significant position to expression of recombinant glycosylated proteins in this mushroom. Human t-PA is a glycosylated protein that degrades the fibrin network formed within blood clots. The gpdII promoter of the button white mushroom strains IM008 and Holland737 were isolated via PCR and the gpdII promoter of the strain IM008 inserted before of hph gene in pCAMBIA1304 plasmid and pCAMBIAH8 constructed. The gLI7 and gLI8 fragments (resulted from connectivity of gpdII promoter of strains Holland737 and IM008, respectively to intron number seven of gpdII gene and laccase1 gene signal peptide) inserted to pCAMBIAH8 and then p13H88 and p13H87 created. Finally the tPA gene cloned to pCAMBIAH88 and pCAMBIAH87 and then p13H88T and p13H87T constructed, respectively. Constructs in this research can be used for tPA production as well as for genetic improvement program of the white button mushroom.

Background and Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis and differentiation of the causative Candida species.
Whole blood samples were collected twice, from 72 patients for Real Time PCR and blood culture assays. The primers and hybridization probes were designed to potentiate the specific sequence of 18S rRNA genes using Light Cycler system and Fluorescence Resonance Energy Transfer (FERT). The patients with hematologic malignancies and bone marrow transplant recipients were evaluated for IC based on the revised European Organization for Research and Treatment of Cancer/ Mycoses Study Group (EORTC/MSG) criteria.
From 2009 to 2011, 72 patients with hematologic malignancies and bone marrow transplant recipients were evaluated for IC. The female to male ratio was 27:45; the mean age was 32.1 years. The most common malignancy in this patient was acute myeloid leukemia (AML) (27.8%) and acute lymphoblastic leukemia (ALL) (26.4%). Out of 72 patients, 11 patients (15.3%) had positive real time PCR /probe results. Based on the melting temperature (Tm) analysis, 5 (45.4%) C. krusei, 3 (27.2%) C. tropicalis, 2 (18.1%) C. parapsilosis and 1 C. albicans (9%) were identified. According to the revised EORTC / MSG, 1 patient (9%) and 10 patients (91%) were defined as proven and possible groups of IC, respectively. The mortality rate in proven and possible IC patient was found 54.5%.
The established Real-time PCR/FRET probe assay is an appropriate diagnostic tool for the detection of Candida species DNA and the management of patients suffering from hematologic malignancies and bone marrow recipient are at risk for IC.

Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Five milliliter blood samples from healthy volunteers were spiked with 100-106 C. albicans cells to determine the detection limit of our method. DNA was extracted from whole blood using glass beads and the QIAamp DNA Blood Mini Kit (Qiagen, Hilden Germany). DNA from C. albicans isolates were amplified with primers and inserted into Escherichia coli (E. coli) DH5α.1 cells with the TA cloning vector (Invitrogen). The plasmid was used for standardization and optimization. A quantitative PCR assay with the LightCycler amplification and detection system based on fluorescence resonance energy transfer (FRET) with two different specific probes was established. To assess the precision and reproducibility of real-time PCR the intra-assay precision was determined in six consecutive assays. No cross-reactivity of the hybridization probes with the DNA of non-C. albicans species or human genomic DNA was observed, which confirmed its 100% specificity. The minimum limit detected was one C. albicans cell or 100 CFU/ml (10 fg) per PCR reaction. The real-time PCR efficiency rate for Candida was high (E = 1.95). Melting curve analysis of C. albicans showed a specific melting peak temperature of 65.76 °C. The real-time PCR assay we developed is highly specific and sufficiently sensitive to detect the fungal load for early diagnosis of invasive candidiasis.