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Stem Cell differentiation is a process composed of vast variety of factors which are controlled by a network of certain mechanisms. This study aims to determine the possible role of DNA methylation, a potent regulator of VHL, ECAD and RUNX3 genes during Erythroid differentiation driven by miR-451.
To determine the methylation status of promoters and the expression levels of VHL, ECAD and RUNX3 genes, Methylation Specific PCR (MSP) and real-time PCR were used, respectively, on both Cord Blood CD34 Hematopoietic Stem Cells and differentiated cells. To measure the expression levels of mir-451, mirna qpcr technique was used.
Our findings demonstrated a similar methylation pattern for the target genes before and after differentiation by miR-451. However, the expression levels were significantly increased after differentiation. Gene expression and surface marker analysis results further confirmed the potential of miR-451 for driving erythroid differentiation from hematopoietic stem cells.
Our findings ruled out DNA methylation effect on the regulation of VHL, ECAD, and RUNX3 genes during miR-451 mediated erythroid differentiation. However, having CpG islands in their promoters, these three genes are candidates to be controlled by methylation which may not able to be detected by MSP method.
Taken together in this study we have shown a successful erythroid differentiation mediated by miR-451 which is at least in part, independent of DNA methylation. Further understanding of the underlying mechanisms driven by eryhtroid differentiation may lead to therapeutic measures to alter disorders of hematopoietic stem cell differentiation.

Early diagnosis and treatment of leukemia patients remains a fundamental aim in clinical oncology, especially in developing country. Present study highlights the basic requirements of these patients in Iran. Better understanding of these issues may lead to improve the healthcare standards toward leukemia diagnosis and treatment. This descriptive study included 101 specialists in hematology-oncology and pathology serving in oncology centers. The participants were then asked to fill out a standard questionnaire on the issues around diagnosis and treatment of blood malignancies. According to specialists, unfair distribution of facilities across the country, delayed diagnosis of disease, absence of psychological support for patients, and insufficient financial support were the main reasons of inappropriate diagnosis and treatment in leukemia patients. Our results show that making an amendment to health policies by preparing well-equipped medical centers in all provinces, improving the morale of patients through consultation during the process of treatment, and above all, subsiding leukemia patients'' financial problems will promote the health standard regarding the leukemia diagnosis and treatment in Iran.