فهرست مطالب n. kalhor

Spermatogenesis is a complex phenomenon that is influenced by various genes. One of these genes that is likely to be effective in causing azoospermia is CLCA4. This study was conducted to investigate the association between rs763763348 and rs190628533 single nucleotide polymorphisms in CLCA4 gene and azoospermia in Iranian men.

In this cross-sectional study, blood samples were collected from 100 men suffering from non‐obstructive azoospermia referred to Jihad Daneshgahi Infertility Treatment Center in Qom, as well as 100 fertile men who had at least one child and had healthy sperm test. The DNA of the samples was extracted by salting out method. Then, Tetra-primer ARMS PCR technique was used to check single nucleotide polymorphisms and the relationship between single nucleotide polymorphisms (SNPs) and male infertility was investigated.

The mean age of healthy people was 33.12±2.789 years and the mean age of patients was 32.54±2.571 years. The mean level of FSH hormone in healthy and sick subjects was 6.86±1.214 and 15.05±2.078, respectively (p<0.0001). The mean level of LH hormone in healthy people (4.12±1.04) and in sick people (11.44±1.54) showed a significant relationship (p<0.0001). In all studied subjects, the genotype of 100% of the subjects regarding rs190628533 was CC and the genotype of 100% of subjects regarding rs763334876 was GG. There was no significant difference between the healthy and sick groups in the evaluation of these two SNPs.

According to the results of this study, single nucleotide polymorphisms of rs190628533 and rs763334876 are not the cause of infertility due to azoospermia among Iranian men.

Biofilms are a community of bacteria on surfaces coated with extracellular polymeric materials, and one strategy to remove biofilms is to use nanoparticles. The aim of this study was to synthesize green zinc oxide (ZnO) nanoparticles and determine its anti-biofilm effects on biofilm gene expression in multidrug-resistant Klebsiella pneumoniae strains.

In this in vitro study, ZnO nanoparticles were synthesized by green method using zinc nitrate solution and Typha latifolia extract. Its physical and chemical properties were confirmed by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and FTIR (Fourier-transform infrared spectroscopy) methods. Its antimicrobial and anti-biofilm effects against multidrug-resistant Klebsiella pneumoniae isolates were investigated using minimum inhibitory concentration and plate microtiter methods, respectively. Finally, the effects of ZnO nanoparticles on the expression of wzm biofilm gene were investigated using Real Time PCR.

The synthesized ZnO nanoparticles had a spherical morphology and had a size of less than 100 nm. The results also showed that the synthesized ZnO nanoparticles had significant antimicrobial and anti-biofilm effects against multidrug-resistant K. pneumoniae strains and these nanoparticles significantly reduced the expression of wzm biofilm gene (p<0.05).

The findings of this study showed that the synthesized ZnO nanoparticles have significant antimicrobial and anti-biofilm effects and in the future, with further studies, these nanoparticles can be used as an anti-biofilm candidate.