فهرست مطالب n. nasiri

Screening of marine bacteria for developing new drugs is an emerging field in marine biotechnology. The purpose of this study was to investigate the diversity of sponge-associated bacteria from Kish and Larak islands (Persian Gulf) and to determine their antimicrobial, antioxidant, and cytotoxic activities. After sampling, bacteria were grown on the marine sponge agar medium. The isolated bacteria were characterized by polyphasic methods. The antimicrobial activity of the isolated bacteria was determined by the microdilution broth method. Cytotoxic activity was evaluated by MTT (3-4,5-dimethylthiazol-2yl-2,5-diphenyltetrazolium bromide) assay on human cell lines. Antioxidant activity was performed by inhibiting DPPH free radicals. Among 121 bacterial isolates, Vibrio and Bacillus genera were the dominant frequency. The minimum inhibitory concentration of the extracted metabolites was recorded in the range of 64 to 512 µg/mL. The IC50 of antioxidant activity varied from 73.42 to 670.90 µg/mL. The cytotoxic activity of the extracted metabolites ranged from 40.57 to 181.80 µg/mL against SW 480 cell line and 141.30 to 359.70 µg/mL against HepG2 cell line. The HL 15, HL 85, and HK 5 extracts showed less toxicity against human umbilical vein endothelial cells. The results of genetic identification based on the comparison of 16S rRNA gene sequence showed that the potent strains HL 15, HL 24, HL 85, HK 5, and HK 36 belonged to B. safensis, V. alginolyticus, V. rotiferianus, B. aureus and Pseudomonas paralactis, respectively. The present study provided a new understanding of the diversity pattern and biological activity of the bacteria associated with Haliclona oculata.

In assisted reproductive techniques, the selection of a good quality embryo is an important goal, which requires access to a standard method. Today, there are several methods for evaluating in vitro embryos. The morphological evaluation is based on the cleavage rate, shape and symmetry of the blastomeres in the day 3 and day 5 of development. Also, in the preimplantation genetic diagnosis (PGD) method, embryos are evaluated for genetic diseases, to finally are selected a suitable embryo for transfer to the mother's uterus. Since it is important to achieve a low-risk, and high-precision method for selecting of good quality embryos. Recently, the evaluation of embryonic metabolism using analyzing of the material absorbed and secreted by embryos in culture medium, as a non-invasive assessment, has been proposed, that can predict the embryo growth and development more accurately along with morphological evaluation. Therefore, in this narrative review study, relationship between embryo metabolism and its quality has been discussed as a new approach.

Anxiety has multiple effects on heart performance; there are various pharmaceutical and non- pharmaceutical methods to reduce anxiety. Since different colors and ambient light have multiple effects on the patients’ anxiety, this study aimed to determine the impact of color and ambient light on the patients' anxiety in the coronary care unit of Jiroft Imam Khomeini Hospital in 2018.

This experimental study was conducted in the coronary care unit in Jiroft, 2018. Participants (240) were divided into four intervention groups and two control groups. The participants were asked for consent then they were randomly divided into groups of intervention and control to compare the effect of orange, purple (intervention groups) and dark blue with sunlight (control group) on anxiety. Data were collected using the Spielberger situational anxiety questionnaire in two stages of hospitalization and discharging for each patient. Data were analyzed using the analysis of variance.

According to the results of this study, there was a significant difference in the average score of hospitalized patients’ anxiety in the purple units (p=0.03) and purple units with sunlight (p=0.01).

The findings of this study showed that using purple and the integration of purple and sunlight in the coronary care unit can be useful in reducing patients' anxiety. Accordingly, it is better to consider the effect of purple and sunlight in the design of the coronary care unit.

Congenital hypothyroidism is one of the reasons for mental retardation and premature death of infants. Since identification of the determinants of hypothyroidism plays a significant role in its prevention, this study was conducted to determine the prevalence of congenital hypothyroidism and to investigate its determinants in newborn infants.

This study was a secondary analysis of the data of the neonatal congenital hypothyroidism screening program. Hypothyroidism was diagnosed based on the Thyroid Stimulating Hormone (TSH) level in the heel prick blood samples on the third to fifth day of life. The data of infants born in Jiroft hospitals were collected from Jiroft Health Center and analyzed using descriptive statistics and Poisson regression test.

In this study, 4998 newborns (2450, 49.02% female, 2548, 98 / 50% male) were investigated. The study samples were newborns born from March to March 2016. The prevalence of congenital hypothyroidism was 1 in 135 live births. The prevalence of congenital hypothyroidism was higher in babies born by cesarean section (IRR = 2.2, 95% CI =1.1-4.1), newborns admitted to the NICU (IRR = 4.6, 95% CI=2.4-8.9), and babies with high birth weight (IRR = 5.3, 95% CI =3.5-8.1).

The prevalence of hypothyroidism was higher in this study compared to other studies. Its prevalence was higher in males than in females. Genetic and environmental differences may explain this difference. The prevalence of hypothyroidism was higher in infants born through cesarean section and newborns hospitalized in NICU.

The present study investigated sexually disrupting effects of a weak estrogen receptor agonist, nonylphenol ethoxylale on 17 ß – estradiolproduction and ovary tissue in immature Zebra Cichlid (Cichlasoma nigrofasciatum). To determine the effects of nonylphenol ethoxylale ( NPEO ) on female sex hormone of Zebra cichlid, the fish were divided in 3 weight groups ( < 4 g , 4 – 5 g and > 5 g ) and exposed to three concentrations ( 10 – 50 – 100 µg / L ) of NPEO in glass aquariums during 60 days. At an estimated NPEO concentration of 50 and 100 µg / L, 17 ß – estradiol production significantly was increased (P < 0.01). Special changes were showed in 50 µg / L. Estadiol levels were 0.13 – 0.70 ng /ml. This evidence of steroid hormone (17 ß – estradiol) levels showed that nonylphenol ethoxylate (>10 µg / L ) could exert estrogenic effects through gene expression and producting enzymes involving vitellogenesis and lead to production of grown oocytes in 50 µg / L of nonylphenol ethoxylale.