najmeh hadizadeh shirazi

Colorectal cancer is among the most common cancers in developed and developing societies. Studies have shown that lifestyle and especially stress can be effective in getting this condition. Considering the key role of norepinephrine in the occurrence of stress and the frequency of use of stress-suppressing drugs such as propranolol, this research aimed to investigate the cytotoxicity of norepinephrine and propranolol on colorectal cell lines by the MTT assay method.

The Caco-2 cell line was purchased from the cell bank of the Pasteur Institute Research Center of Iran. After cell culture, it was treated with serial concentrations (0-100μg/mL) of propranolol and norepinephrine for 24, 48, and 96 hours, and the toxicity of the treatments was evaluated with the MTT assay test, compared to the control group.

After 24 hours of cell culture with the treatments, there was no significant change in the growth rate of the cells. After 48 hours, only propranolol at a concentration of 30.11 μg/mL could lead to 50% mortality. After 96 hours, propranolol with a concentration of 3.42 μg/mL and norepinephrine with a concentration of 91.1 μg/mL showed 50% growth inhibition.

According to the data obtained from the research, it seems that although both additives had a toxic effect on the cells, propranolol had more toxicity on Caco-2 colorectal cancer cells than norepinephrine. Further studies are suggested in the field of new clinical applications for propranolol.

The present study aims to evaluate the possible amelioration of the toxic effect of Pb2+ on the liver and kidney organs of zebrafish (Danio rerio) by the probiotic strain Lactobacillus fermentum as a dietary supplement.

Zebrafish that had beedn treated with probiotics for 60 days were exposed to 0-1500 μg/L Pb2+ for 72h. The LC50 for the control group was determined, and biometric, histological, and antioxidant biomarkers were assessed. This included lipid peroxidation, SOD (superoxide dismutase) activity, reactive oxygen species (ROS) levels, and inflammatory response, focusing on interleukin-8 (IL-8) and interferon-gamma (IFN-γ) gene expression.

Based on morphological analysis, the probiotic-enriched diet had no significant effect on the weight and length of zebrafish as biometric parameters. However, it reduced tissue heavy metal accumulation and mortality by 83% at 1500 μg/L Pb2+ after 72 hours of T2 treatment. Tissue damage, including hepatocyte necrosis, renal and tubular degeneration, decreased significantly in treated groups. Oxidative stress analysis revealed that probiotic treatment alleviated free radical production, SOD activity, and malondialdehyde (MDA) levels in zebrafish, allowing them to tolerate more than 300 μg/L Pb2+. In addition, probiotic-treated zebrafish showed a significant recovery in SOD2, IL-8, and IFN-γ mRNA expression, indicating an improved immune system.

In conclusion, a diet enriched with Lactobacillus fermentum reduced the oxidative stress and improved the resistance of zebrafish to the sub-lethal dose of Pb2+. Overall, Lactobacillus fermentum shows potential as a dietary supplement for aquaculture in Pb2+-contaminated areas.

 Due to the growing need of industries for raw materials and high environmental hazards of chemical products, many researchers and manufacturers have turned their attention to the use of biological products, especially products of microbial origin. In this study, the potential of Aspergillus oryzae and Aspergillus flavus for resistance to ultra violet light in the range of 350-290 nm has been evaluated.

two species of fungi Aspergillus oryzae and Aspergillus flavus, after being purchased as lyophilized powder, were cultured in various culture media for 10 days to select the appropriate culture medium to produce the maximum biomass. Samples were cultured in selective culture medium, PDA, for 7 days and after drying the biomass and separation of pigments, the amount of SPF parameter and the survival of colonies on the culture medium in the presence of ultraviolet rays were measured.

Both types of fungi can withstand 350 nm for about 20 minutes, but the resistance of the fungi to light is 290 nm is much lower. Also, the SPF of Aspergillus flavus is higher than that of Aspergillus oryzae. Thus, it can be concluded that the pigments of Aspergillus oryza and Aspergillus flavus have shown good protection against ultraviolet rays. Therefore, these natural pigments can be used as alternatives to chemicals in industry.

the pigments of Aspergillus fungi studied have shown good protection against ultraviolet rays and have good potential for use in industry.

Biological samples such as bones, tooth, and hair obtained from archaeological excavations are mainly used for dating measurements. However, these findings provide valuable information about the diet, lifestyle or migration, and genetic interaction of communities, and disease because the biochemical content of biological tissues such as bones, teeth, and hair changes under the influence of the lifestyle of the past population. Therefore, it seems that human remains' biochemical, microscopic, and histological studies could be advantageous for clarifying the dark events of the past societies. Bioarchaeology, as an interdisciplinary science, assesses the historical-cultural relationships in ancient samples and human remains like hair, bone, or tooth by biological tools and techniques. In this science, the biological data of specimens are combined with archeological information to obtain a more accurate sociology of past civilizations. The purpose of the present review article is to familiarize archeology researchers with the usual methods in bioarchaeological studies.

In the present study, the position of aromatic amino acids as important elements in the formation and stability of the tertiary structure of zinc-denatured peroxidase has been investigated with the help of second-derivative graph analysis of spectroscopic data. For this purpose, the second- derivative of absorption and emission graphs with 10 replications in the visible UV range of circular dichroism and fluorescence spectroscopy for the enzyme in the presence of 500-100 μM zinc chloride was prepared and studied with the help of MATLAB software. The results show that zinc ions reduce the compaction of microstructures around aromatic amino acids, especially for tryptophan. However, even at a concentration of 500 μM zinc, tryptophan is not directly exposed to the hydrophilic solvent. In general, tryptophan seems to be more important than other aromatic amino acids in inhibiting the enzyme by zinc metal.

Many cellular phenomena, such as the onset of cell division or the polymerization of cytoskeletal microtubules, are controlled not only by chemical or electrical connections, but also use long-distance methods such as sending electromagnetic waves. The article is a brief overview of past research and new information and documents published in this field.

This review article has been performed by searching electromagnetic waves, cell, cellular connections, cell interaction, cellular biophysics keywords in various data bases such as Scopus, Science Direct, and Google Scholar. Results Studies have shown that the presence of electromagnetic waves is directly involved in the initiation and conduction of phenomena such as mitogenetics, mirror cytopathy or death flash and indirectly in other cellular processes.

In recent years, research has shown that many cells influence each other’s behavior by sending electromagnetic waves together. The existence of such connections can justify many unknown cellular behaviors.

Aim and Zinc is one of the metals that preserve the structure and function of many tissues in the body. Its effect is concentration-dependent. It can disrupt the vital process of cellular respiration. This study is aimed to investigate the interaction of this metal in the function and structure of peroxidase as one of the most important oxidoreductase enzymes in cellular respiration process, experimentally and theoretically.
Material and In order to extract kinetic parameters using UV-visible spectroscopy techniques and molecular docking modeling, horseradish peroxidase activities were plotted against time and various concentrations of zinc. Absorbance spectrum of the enzyme was obtained in the presence and absence of zinc, to determine the structural change, which are responsible for enzyme inhibition..
The results showed that Zn2 strongly inhibited peroxidase activity in a reversible uncompetitive-noncompetitive pattern with IC50 370 μM. Moreover, Zn2 enhanced the lag time and decreased steady state rate of the enzyme. UV-visible spectrum and molecular docking analysis indicated that Zn2 binding site is distinct from the active site and transformational change and reduction of electron transfer ability of porphyrin ring were occurred in the presence of Zn2. .
We propose that excess amount of zinc induces inhibition in peroxidase activity and possibly causes disorder in cellular respiration and electron transport. It is also believed that it could be useful in drug design for cancer cells.

An amperometric glucose biosensor was developed based on synergistic contributions of PB and a bucky gel (BG) consisting of carbon nanotubes (CNTs) and ionic liquid (IL). The PB nanoparticles were first deposited onto the surface of a BG modified glassy carbon (GC) electrode (BG/GC). Then, the Ni2 ions were electrochemically inserted into the PB lattice to improve its stability in physiological pH. Afterwards, Glucose oxidase (GOx) was immobilized on the BG/GC electrode using a cross-linking method. Amperometric measurements of glucose were performed at −0.05 V vs. Ag/AgCl in 0.05 M phosphate buffer solution at pH 7.4. The glucose biosensor exhibited a sensitivity of 45.03 µA mM−1 cm−2 with a detection limit of 5×10-7 M. The amperometric response was linear in the range of 5×10-7 to 8.3×10−4 M.