فهرست مطالب negar khorasani

Pancreatic cancer is one of the most deadly and aggressive cancers; Fluorouracil induces apoptosis and cell cycle arrest in cancer cells. In the present study; the effect of Fluorouracil on different stages of the cell cycle and the expression of genes involved in the internal pathway of apoptosis in the AsPC-1 cell line (human pancreatic cancer) were investigated. In order to do so, MTT assay was used to evaluate the cytotoxic effect of Fluorouracil on AsPC-1 cell proliferation; The type of induced cell death and cell cycle changes were investigated by flow cytometry; changes in the expression level of genes (BAX, Bcl-2, APAF-1, Caspase-3, Caspase-9, p53, p21) were examined by Real-time PCR. Quantitative data were analyzed at the significant level of (p<0.05). The MTT assay results showed that Fluorouracil decreased AsPC-1 cell proliferation in a concentration-dependent manner. The results of flow cytometry analysis showed that increased percentage of apoptotic cells in the treated cells; Fluorouracil induces S phase cell cycle arrest in AsPC-1 cells and reduced distribution in the G1 phase. The Real-time PCR results in treated cells showed an increase in the expression of genes in the mitochondrial apoptotic pathway as well as genes effective in regulating the cell cycle. Fluorouracil reduces cell proliferation and induces apoptosis by increasing the expression of genes involved in the Intrinsic apoptotic pathway in AsPC-1 cells; Fluorouracil also caused cell cycle arrest in these cells by regulating the (p53, p21) genes.

Since Covid-19, as a new and completely complex and anonymous phenomenon, entered the world, cities have not been able to meet the needs of their habitants and people could not change their lifestyle and adapt a new one. In the meantime, the use of new information and communication technologies and the creation of smart urban environments have caused natural and unnatural crises. The aim of this study is, therefore, to evaluate the effect of the Covid-19 outbreak on urban management intelligence, evaluate the impact of corona outbreak on the activities of Mashhad Municipality, and examine the measures taken in the field of intelligence to deal with this crisis. This research is applied in terms of purpose and exploratory-analytical in terms of method. It has adopted a combination of qualitative and quantitative methods (mixed-methods). Data has been collected through library and field studies. The statistical population of the research in the first level included 50 elites and available experts selected through snowball sampling and in the second level, 200 citizens have been selected for field analysis. Mick Mac Software and Scenario Wizard were used to analyze the extracted information. The research indicators included intelligent transportation, smart economy, smart government, smart living, smart citizens and smart environment. The results of the research indicated 8 key factors with several possible situations and scenarios for the future of urban management intelligence in the city of Mashhad.

Today, development is a concern of many countries with the aim of improving the quality of human life. In order to achieve the desired development pattern or the correct use of resources and opportunities and the fair distribution of facilities, people's participation is the main and pivotal strategy. Accordingly, the present study examines and evaluates the feasibility of indicators of active public participation in line with the Islamic-Iranian model of progress.

The type of applied-comparative research and research method is content analysis and library and field information methods have been used in conducting research. In the field method, a questionnaire technique was used to collect information. The statistical population of the study includes 50 elites and available experts. SPSS statistical software and one-sample t-test and Kruskal-Wallis statistical tests were used to analyze the questionnaire data and also the SWOT strategic model was used to determine strengths, weaknesses, opportunities, and threats.

The results of t-test showed that there is a significant relationship between the independent variables studied in the present study and the feasibility of the Islamic-Iranian model of progress. Therefore, the studied independent variables affect the feasibility of the model. Also, the results of Kruskal-Wallis test have shown that the tendency of people to participate in relation to the indicators and variables measured in order to achieve the model is in a favorable position. The results of the SWOT model in relation to the status of the pattern as well as the scoring of the relevant factors indicate the choice of offensive strategy in the direction of action.

Silver nanoparticles (AgNPs) have antibacterial, anti-angiogenesis and anti-cancer activity. The purpose of this study was to examine the apoptotic effects of AgNPs coated with Zataria multiflora leaves extract on HepG2 liver cancer cells. HepG2 cancer cell lines were treated with different concentrations of AgNPs for 24 and 48 hours. The viability of the cells and inhibitory concentration (IC50) were determined by the methylthiazol tetrazolium (MTT) assay. To evaluate the ability of AgNPs to induce apoptosis in HepG2 cells, the 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI), acridine orange-propidium iodide staining and flow cytometry analysis for annexin V- propidium iodide were used. The results of the MTT assay showed that AgNPs decreased the proliferation of HepG2 cells in dose- and time-dependent manner. The IC50 during the incubation times of 24 and 48 hours were determined 40 and 30 μg/mL, respectively (P<0.05). The results of DAPI staining showed that AgNPs can lead to break down of nuclear DNA. Moreover, the acridine orange-propidium iodide staining and annexin V-propidium iodide showed that the percentage of apoptotic cells was increased in the treated cells. Silver nanoparticles covered with Z. multiflora leaf extract have the ability to induce apoptosis in cancer cells HepG2 and they can be considered as a promising strategy for the treatment of liver cancer.