فهرست مطالب p. maskulrath

Community domestic wastewater contains organic substances that can be decomposed through natural processes. Treatment using oxidation pond systems is popular in tropical zones because these locations provide a climate suitable for the growth and organic decomposition activities of various bacteria that remove organic contaminants from wastewater. Given that bacteria play an important role in the biodegradation of organic substances in wastewater treatment plants, their degradation activity is used as an indicator of water quality. The purpose of this study is to examine the vertical variability of bacteria in natural treatment oxidation ponds in tropical zones. Wastewater samples were collected from an oxidation pond at 3 different depths (0–0.6, 0.6–1.5 and more than 1.5 meter), and their chemical, physical and biological qualities were analysed. Next-generation sequencing techniques were used to identify bacterial diversity, and the water quality at each depth was applied as an indicator of bacterial degradation activities. Community domestic wastewater contained 10 major bacterial phyla that differed at different depths. Amongst these phyla, the Actinobacteriota dominated (25.35 to 28.23 percent), followed by Cyanobacteria (19.49 to 21.57percent), Planctomycetota (15.50 to 17.41 percent), Firmicutes (9.97 to 10.79 percent), Proteobacteria (9.73 to 10.79 percent), Verrucomicrobiota (6.47 to 7.69 percent), Chloroflexi (2.79 to 2.99 percent), Bacteriota (0.96 to 1.41 percent), Acidobacteriota (0.70 to 0.80 percent) and SAR324 clade (marine group B) (0.69 to 0.61 percent). Four organic substances were found in contaminated domestic wastewater. 1) Photosynthetic cyanobacteria and phytoplankton performed aerobic degradation and accounted for the dissolved oxygen levels of 7.76, 7.45 and 7.42 milligrams per liter, respectively at various depths along the vertical profile. 2) Bacteria and archaeans that participate in carbon compound treatment included Planctomycetes, Verrucomicrobiota, Bacteroidota and Euryarcheota. These bacteria exhibited a treatment efficiency for biochemical oxygen demand and low abundance at all depths. Biological oxygen demand increased to 23.11, 24.27 and 34.48 milligrams per liter with depth. 3) Nitrogen-cycling bacteria included nitrifying and denitrifying bacteria. They belonged to the Actinobacteriota, Planctomycetota, Firmicutes, Verrucomicrobiota, Chloroflexi, Bacteriota, Protrobacteria and Acidobacteriota phyla. They exhibited an ammonia-nitrogen treatment efficiency of 91.73 percent. 4) Phosphorus cycling–related bacteria in the Actinobacteriota phylum presented the orthophosphorus treatment efficiency of 65.41 percent. The results of this study suggested that bacterial communities did not significantly differ along oxidation depth because they work together with chemotrophs, which participate in organic substance decomposition, and phototrophs, which are involved in oxygen generation and nutrient removal. All of the bacteria found in domestic wastewater benefit and support oxidation pond systems in tropical zones. Anaerobic bacteria can be found in wastewater and used in treatment systems due to their protective mechanisms against oxygen toxicity and self-repair mechanisms. The knowledge gained from this study can be used as a reference in future works on natural wastewater treatment systems.

Domestic wastewater pollution in Thailand presents challenges due to limited space and a high concentration of point source effluents. This phenomenon often leads to domestic wastewater exceeding the capacity of local treatment systems. This study aims to expand the knowledge gained from The King’s Royally Initiated Laem Phak Bia Environmental Research and Development Project by evaluating the treatability of municipal wastewater. It utilizes a constructed wetland system in conjunction with a transfer and point source system. After the implementation of this primary system, the reduction in highly contaminated domestic wastewater could enhance the treatment loading of other secondary treatment systems or even facilitate its release into natural pathways. In the sampling collection process, the dynamics of the collection points were categorized into three different zones: 1) the point sources of domestic wastewater within a municipality, where 15 sample points were selected to represent the municipality; 2) the collection pond within the municipality and the transfer pipeline, comprising three collection points of the system; 3) the constructed wetland treatment system, where five water samples were collected in relation to the length of the existing 100-meter plot. The water samples were collected using four 1-liter polyethylene bottles. The analysis parameters were the biological oxygen demand, total nitrogen, nitrate, total phosphorous and phosphate, and other parameters related to domestic wastewater treatment efficacy. This study reveals that the domestic wastewater in Phetchaburi Province initially has a high organic content, leading to a biochemical oxygen demand: nitrogen: phosphorous ratio of 100:2.5:0.2 favoring anaerobic degradation. This ratio shifts in the constructed wetland system, located 18.5 kilometers away, to 100:10.5:2.3, promoting anaerobic treatment. The system shows high efficacy, with 81.4, 50.0, and 58.3 percent removal rates for biochemical oxygen demand, nitrogen, and phosphorus, respectively. This efficacy corresponds to a notable reduction in average biochemical oxygen demand from 740.0 to 9.7 milligrams per liter. Moreover, changes are observed in total nitrogen content, shifting from 20.8 to 2.8 milligrams per liter, in the system’s effluent. While lastly, the total phosphorous decreased from 2.75 to 0.60 milligrams per liter This treatment method can be effectively applied to small-scale constructed wetland systems within households. The recommended hydraulic retention time is between 29 and 60 hours under anaerobic conditions and 3 days under aerobic conditions. The changes in the composition of municipal wastewater, which is highly organic, support the use of both degradation processes. The knowledge and application of the constructed wetland system could be suggested for the primary treatment system of domestic wastewater within municipalities, given that this system would provide support to the central wastewater treatment system for enhanced efficacy.

Landslide disasters in Thailand between 1970 and 2011 revealed a notable pattern: they primarily originated on mountain slopes, distinguished by a deeper soil profile. This soil profile comprised clay loam and sandy loam textures and was situated over aged geological formations of granite and shale rocks. The affected areas included the southern and northern provinces of Thailand.This study investigated the consequences of landslide hazards on stream water and sediment quality in two watersheds: the Mae Phul–Mae Prong watershed in Uttaradit province, the northern part of Thailand, and the Klong Kram watershed in Surat Thani province, the southern part of Thailand. These watersheds had experienced recurrent landslides, primarily on mountain slopes characterized by deep clayey and sandy loam soils over old granite and shale rock types as well as old granite limestone. During wet and dry periods in April and November 2015, 108 samples were collected from 18 stations (9 stations in the Klong Kram watershed and 9 stations in the Mae Phul–Mae Prong watershed). These samples included upland soil, stream water, and sediments. For upland soils, 1 kilogram samples were collected through auger and V-shaped pit techniques using a stainless-steel spade, with composite sampling conducted at 0–30 centimeters across all 18 stations. Stream water was collected in one part using a 1-L polyethylene bottle at 30 centimeter from the stream layer, while other samples were compositely collected in sterilized glass bottles to determine coliforms. Soil and sediment samples were compositely collected from the bottom using a stainless-steel spade. All samples were stored at 4 degrees Celsius and transported to a laboratory for analysis. The insight gained from these collection efforts elucidated the dynamics of landslide impacts at the spatial scale for the two watersheds. Most water samples met Thai surface water quality standard for various parameters; however, microbial contamination of the water samples attributed to community activities along stream banks was detected. Notably, arsenic was consistently detected in upland soil, stream water, and sediment samples. For Uttaradit, the average arsenic concentrations were 0.22 ± 0.09 milligram per kilogram, 0.01 ± 0.14 milligram per liter, and 9.74 ± 4.42 milligram per kilogram in upland soil, water, and sediment samples, respectively. For Surat Thani, arsenic concentrations were 87.63 ± 208.83 milligram per kilogram, 0.01 ± 0.01 milligram per liter, and 19.44 ± 36.38 milligram per kilogram in upland soil, water, and sediment samples, respectively, particularly near landslide scars where the arsenic concentrations were significantly higher in sediments and upland soils compared with stream water, highlighting the role of landslides near streams. These data suggest that sediment transport from upland soil in the landslide scar into stream water affects water quality, particularly in terms of arsenic concentration near the landslide scar, often surpassing natural standards. The study concluded that stream water was directly affected by landslides as these watersheds were unsuitable for consumption due to arsenic and microbial contaminations. This conclusion emphasizes the critical need to incorporate landslide hazard considerations into watershed management practices to safeguard downstream communities and preserve water resources.

As a producer within the ecological food chain, phytoplankton provides the base energy and oxygen to the environment through photosynthesis and higher tropic levels. These benefits can be applied in five consecutive nature-by-nature oxidation ponds for the treatment of community wastewater coming through a high density polyethylene pipeline from the Phetchaburi Municipality located at the King’s Royally Initiated Laem Phak Bia Environmental Research and Development Project, Ban Laem District, Phetchaburi Province (Universal Transverse Mercator 47P 1442725 North 617774 East). This study focuses on the vertical distribution of the phytoplankton Cylindrospermopsis sp. and its relationship with nitrogen compounds in oxidation ponds.

Samples were collected from a community wastewater treatment system at various depths (30, 60, 90, 120, and 150 centimeters) below the water surface in April 2019 between 11:00 and 13:00 hours and analyzed for their chemical and physical properties. The analysis revealed a vertical relationship between Cylindrospermopsis sp. and wastewater. In the density of phytoplankton which were collected by measuring 20 liters of water and filtered using a 36-micron plankton net, calculated and counted under a high magnification microscope, as the species are classified according the taxonomy.

The results of the wastewater quality were as follows: the content of suspended solids was 65–81 milligram per liter, water temperature was 31.8–33.2°C, potential of hydrogen was 8.7–9.2, total nitrogen content was 4.0–5.3 milligram per liter, ammonium content was 0.03–0.06 milligram per liter, nitrate content was 0.09–0.12 milligram per liter, total phosphorus content was 0.9–1.3 milligram per liter, and phosphate content was 0.4–0.5 milligram per liter. In the density of phytoplankton, a significant correlation was observed between the population of Cylindrospermopsis sp. and water depth (R2 = 0.9324). The number of populations at the depths of 30, 60, 90, 120, and 150 centimeters were 3.2 × 107, 1.6 × 107, 1.1 × 107, 5.5 × 107, and 1.1 × 108 cells per cubic meter, respectively.

The different densities of Cylindrospermopsis sp. found at different depths throughout the treatment pond are related to the nitrogen dynamics of the water body. The results of this study revealed that organic nitrogen, including ammonium, was assimilated and converted to inorganic nutrients, which promoted the growth of other phytoplankton species. The correlation between Cylindrospermopsis sp. and total nitrogen and ammonium showed significance at R2 = 0.7268 and 0.797, respectively, with a confidence level of 0.05. Therefore, to ensure treatment effectiveness, the depth of wastewater treatment ponds should be considered during their construction because phytoplankton regulation plays an important role to maintain the overall treatment efficiency.