parvin ghaderi

Endometrial hyperplasia is the irregular proliferation of endometrial glands and an increase in the ratio of gland to stroma compared to normal proliferative endometrium. Endometrial hyperplasia is one of the precursors of endometrial cancer, which is one of the most common cancers among women. The present study was conducted with the aim of investigating changes in the expression level of FOXO1 and P27 kip genes in endometrial hyperplasia tissue samples compared to normal tissue sample. In this case-control study, samples of hyperplasia with normal marginal tissue were taken from the uterine endometrium of women who referred to the women's clinic of Arash Hospital with abnormal uterine bleeding or increased endometrial thickness. Marginal normal tissue sample and hyperplasia sample were considered as control and case, respectively. The relative expression level of FOXO1 and P27 kip genes was measured by real time PCR method after total RNA extraction and cDNA synthesis, and then the data obtained from the reaction was analyzed by RQ=2-ΔΔCT method. Data were analyzed using independent t-test. The relative expression level of FOXO1 and P27 kip genes in the hyperplasia tissue sample was not significantly different compared to the control group (P>0.05.( Although the results of some studies indicate that the expression level of FOXO1 and P27KIP genes can be changed in endometrial cancer, the results of the present study showed that, contrary to expectations, there is no significant change in relative expression level of FOXO1 and P27KIP genes in hyperplasia tissue compared to normal tissue.

This study was carried out to investigate the hepatoprotective effect of deuterium depleted water with Satureja rechingeri essential oil on liver damage. For this purpose, the adult male rats were divided into 16 groups. The negative control group used tap water in 14 days following DMSO i.p injection at day 15th. The treatment group with acetaminophen received tap water in 14 days following 500mg/kg b.w. i.p injection of acetaminophen dissolved in 300ml DMSO at day 15th. The treatment groups receiving deuterium depleted water (30ppm and 60 ppm) plus i.p injection of Satureja rechingeri essential oil following 500 mg/kg b.w. acetaminophen injection at day 15th.Then, at 4,8,16 and 24 hours after acetaminophen injection, the blood was taken from hurt puncture and the liver tissues were removed. Then, the biochemical marker levels such as AST, ALT, ALP and bilirubin were measured in plasma. The glutathione s-transferase enzyme activity and protein level, cytochrome P450 protein level and glutathione level were also estimated in liver homogenate. Findings indicated no significant changes in the level of serum parameters such as ALT, ALP and bilirubin in groups. While injecting acetaminophen decreased the levels of GST and GSH and increased significantly the level of CYP450 and AST. Treatment with deuterium depleted water and Satureja rechingeri essential oils are effective in moderating the levels of these parameters, leaded to returning of these parameters to normal values. Finally, the treatment of rats with high dose of acetaminophen caused liver oxidative damage and using of deuterium depleted water and Satureja rechingeri essential oil can be effective in preventing and improving these damages.