فهرست مطالب pedram moayeri

Resistin is a hormone secreted from adipose tissue that plays an important role in regulating energy homeostasis and glucose metabolism. In humans, only a small amount of resistin is expressed in adipose tissue and is most commonly found in the bone marrow, spleen, lung tissue, and placenta. The purpose of this study was to investigate the effect of oral administration of selenium and selenite sodium nanoparticles on the transcription rate of the resistin gene in pregnant ewes. For this, 20 pregnant ewes were randomly selected and divided into four groups and were administered daily for 10 days leading up to birth. In treated group 1 sodium selenite (0.1 mg/kg body weight), while in treated groups 2 and 3 selenium nanoparticles (at doses of 0.05 and 0.1 mg/kg body weight, respectively) were administered. The fourth group was received distilled water and served as a control group. At the time of parturition, samples were taken from the placenta and the transcription rate of the resistin gene was determined by RT-PCR Real-Time based on a comparison assay of 2-ΔΔCt. The results showed that oral selenium administration to pregnant ewes caused a significant increase in the amount of resistin gene transcription rate which the role of sodium selenite was more pronounced than that of selenium nanoparticles (With p, equal to 0.002, 0.002 and 0.001 respectively).

n recent years, special attention has been paid to insulin resistance mediators, such as adiponectin, that is produced and expressed during conception in placenta tissue, thereby inducing its effects on the growing embryo and the health of the mother. In this study, for the first time, Twenty four-month pregnant ewes within the same age were selected randomly. During the 21 days leading up to birth, oral administration of selenium nanoparticles (Se NPs) with dosages of 0.05 and 0.10 mg/kg B.W. and sodium selenite with dosage of 0.1 mg/kg B.W. was carried out. At the same time the control group was fed distilled water in equal volume. With sampling of the placenta during childbirth, transcription amount of adiponectin gene was determined by RT PCR Real Time based on a comparison assay of 2-ΔΔCt. The results showed that the addition of selenium supplements to the diet of ewes of pregnant caused a significant decrease in the amount of adiponectin gene transcription, and the selenium nanoparticle suppression was significantly superior to sodium Selenite.

Leptin as a cytokine-like hormone is derived from ob gene (one of the major effect genes on birth weight and growth traits) and is secreted by adipose tissue. This hormone with binding to its receptors in the hypothalamus, inhibits food intake and increases energy consumption. There is not any report about expression of leptin gene in response to oral administration of selenium in livestock. In the present study, the effects of selenium nanoparticle and sodium selenite on the transcription of leptin gene in placenta were studied. Twenty, four-month pregnant ewes within the same age were selected randomly. During the 21 days leading up to birth, oral administration of selenium nanoparticles (Se NPs) with dosages of 0.05 and 0.10 mg/kg B.W. and sodium selenite with dosage of 0.1 mg/kg B.W. was carried out. At the same time the control group was fed distilled water in equal volume. With sampling of the placenta during childbirth, transcription amount of leptin gene was determined by RT PCR Real Time based on a comparison assay of 2-ΔΔCt. The results of this study showed that leptin gene is expressed in placental tissue. The oral administration of selenium nanoparticle and sodium selenite caused a significant increment in terms of expression of mentioned gene in comparison to the control treatment. Also, there was a significant difference between the supplements, so that the highest leptin gene expression in placenta was observed in selenium nanoparticle treatment with dose of 0.1 mg and then supplement with selenium nanoparticles with dose of 0.05 mg. Selenium causes an increment of leptin gene expression in placental tissue.

Streptococcus iniae has emerged as an important fish pathogen over the last decade in farmed rainbow trout in Iran. The main objective of this study was to evaluate the pathogenicity of S. iniae in Poecilia reticulata. Atotal of 60 apparently healthy P. reticulata were obtained from ornamental fish pet store and injected intraperitoneally with 1.5×106 cfu of bacteria. For 14 days after challenge, the rate of mortality and clinical signs were recorded. The first clinical signs was observed in challenged fish 48 hrs after injection of S. iniae and first mortality was observed 72 hrs after injection. No significant differences in mortality and clinical signs between both sexes were observed. Streptococcus iniae was collected from internal organs of fishes challenged, and was confirmed using the conventional biochemical tests and PCR. It is concluded that, P. reticulata is susceptible to streptococcosis and can play an important role in transmission of the disease to other ornamental fish species and also cultured fish.