فهرست مطالب pouria motamed chaboki

We detected a novel strain of picornavirus from dead backyard ducks from Gilan province, In Iran. This new isolated was demonstrated a genome like picornavirus design, specifically Aalivirus (Avihepatovirus/Avisivirus-like virus). Usually, Aalivirus were observed in domestic ducks, turkey and chickens. Ten pooled feces samples were collected from samples. We implemented partial genome sequencing of VP1 gene. We nominated the new strain as duck Aalivirus isolate UT-Ebrahimi. Based on our investigation with phylogenetic tree, we considered this strain is a member of the Aalivirus genus of picornavirus. In this study, we demonstrated that the UT-Ebrahimi is 96.23 % similar to Pacific_black_duck_megrivirus_(MK204391.1).

Avian influenza disease is one of the most significant contagious diseases that can infect many birds, either in commercial farms or in indigenous birds. There have been many reports on the highly pathogenic H5 subtype of avian influenza outbreaks from Iran's industrial poultry farms in recent years. Anseriformes, as the main reservoir of the virus, play a critical role in the epidemiology of the disease. This study was conducted to detect antibodies against the H5 subtype of avian influenza viruses in the yolk of native ducks. Seventy duck eggs were collected from different cities of Gilan province (Rasht, Anzali, SoomeSara, Fuman, Astaneh Ashrafie, Langroud und Chaboksar) from April to September of 2020. The aquatic phase of eggs was separate and subjected to the OIE approved HI standard test using H5 specific antigen. 11.43% of tested samples showed antibodies against the H5 subtype of influenza virus. This study demonstrates the circulation of the H5 subtype viruses among the native duck population of the north of Iran.

Avianmetapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and swollen head syndrome in chickens. Four aMPV subgroups (A-D) have been reported previously based on their genetic and antigenic differences. Evidence suggests that the live bird markets (LBMs) play an important role in the epidemiology of the avian viral diseases. A total number of 450 oropharyngeal samples from eight different species of birds (migratory and local) were collected from LBMs of Gilan province, Iran, from October to December 2016. The presence of aMPV was determined by reverse transcription polymerase chain reaction (RT-PCR) based on nucleoprotein gene. The aMPV was detected in 30.60% of the examined birds including chickens (37.00%), turkey (33.00%), Eurasian teal (25.00%), common blackbird (33.00%), and Eurasian woodcock (25.00%). Bioinformatics analysis and a phylogenetic tree based on partial nucleotide sequences of the N gene showed that the detected aMPVs were belonged to subtype B. This is the first report of aMPV in non-commercial birds in Iran. Knowledge of the frequency and types of infected birds with pneumoviruses allow a better understanding of the epidemiology of aMPV in Iran.