pu wang

This is the first study on phylogenetic relationships in the genus Artemia Leach, 1819 using the pattern and sequence of secondary structures of internal transcribed spacer 1 (ITS1). Significant intraspecific variation in the secondary structure of ITS1 rRNA was found in Artemia tibetiana. In the phylogenetic tree based on joined primary and secondary structure sequences, Artemia urmiana and parthenogenetic populations displayed new lineages, and two New World species (Artemia franciscana and Artemia persimilis) were located in a basal clade that was not detected in previous studies. The close evolutionary relationship between A. franciscana and A. persimilis are expressively supported by the previous empirical and experimental investigation on the ability of hybridization (in natural habitats and lab conditions) and analysis on allozyme markers.

The aim of this study was to observe the effect of intensive statin therapy on symptomatic intracranial arterial stenosis.
overall, 120 patients with symptomatic intracranial arterial stenosis were admitted to the Xiangyang No.1 People’s Hospital, Hubei University of Medicine, Xiangyang, China from January 2010 to May 2013. They were randomly divided into three groups and were given different doses of atorvastatin orally for 1 year or more, and followed up for 12 months. The three groups were assessed for clinical end-point event rates and changes in cerebral blood flow value before and after treatment to assess the effectiveness of intensive statin therapy.
The incidence rates of end-point cerebrovascular events in the low-dose group (10 mg/d), the general-dose group (20 mg/d) and the intensive treatment group (40 mg/d) were 26.3%, 13.5% and 5.4% respectively during the 12-month follow-up after treatment. There was a significant difference between the low dose group and the intensive treatment group (P Atorvastatin at 40 mg/d has a significant advantage compared with atorvastatin at 20 mg/d and 10 mg/d in reducing cerebrovascular events and improving cerebral blood flow.

The antifungal activities of chitosan with different molecular weights and concentrationsagainst Aspergillus niger were studied in vitro. The results showed that the antifungal activity of chitosan against Aspergillus niger is molecular weight and concentration dependent. The smaller the molecular weight, the stronger would be the antifungal activity. Chitosan with higher molecular weight and concentration has no antifungal but promotion activity towards Aspergillus niger. It is only the chitosan with proper molecular weight and concentration which possesses preferable antifungal activity towards Aspergillus niger. The effects of chitosan on Aspergillus niger and hyphal ultrastructure were examined to gain more information on its mode of action. The ultrastructure morphology investigated by transmission electron microscopy results indicated that chitosan acts on Aspergillus niger by inhibiting the growth of sporules. The fluorescein isothiocyanate labelled chitosan observation has elucidated the antifungal activity of chitosan to be caused mainly by inhibiting the DNA to RNA transcription. So the antifungal activity of chitosan towards Aspergillus niger was the combined effect of the above two observations. The study has provided sufficient scientific evidence for careful application of chitosan in food and medical industry.