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Acne vulgaris is a pilosebaceous gland illness that often affects people from puberty to early adulthood as a result of hormonal changes; however, the specific association of various hormones to acne is unknown. The current study aims to discover the relationship between hormonal status and acne vulgaris patients.Incases, one hundred patients with Acne Vulgaris aged 15-30 years were included. Fasting blood samples were taken from patients prior to the start of treatment.After presenting the contents of the study, all individuals in the cases and control groups provided written and informed consent.Acne vulgarispatients' levels of Luteinizing Hormone (LH), estrogen, Follicle Stimulating Hormone (FSH), and Testosterone were compared to control groups. The T-testwas used for statistical analysis. Conclusion Levels of LH, Estradiol, and Testosterone can be measured in acne sufferers, however,FSH levels do not need to be measured.