فهرست مطالب reyhaneh behtaj

Breast cancer is a heterogeneous disease in which many factors and receptors are effective in the disease process and response to treatment. Currently, estrogen, progesterone, and HER2 receptors are among the most important factors in choosing a treatment regimen. Other metabolic factors that may affect the treatment outcome include diabetes and hyperinsulinemia. In order to evaluate the role and complexity of cross-talk between different pathways initiating from various receptors, value the most common drugs in the treatment of breast cancer are investigated on different cell lines in this manuscript at the cell culture level. The result of different doses of Tamoxifen and estradiol on the cells with various levels of the estrogenic, progesterone, and HER2 receptors is examined alone, or in combinations, and the presence or absence of insulin. The effects of these variables on the cells’ growth pattern and survival in various breast cancer cells are investigated using cell counting, colony counting, and MTT assays. Our results have further confirmed the complexity of deciding on the outcome of treatment for breast cancer with such a wide variability in the kind of receptors and biochemical agents present in the body of a cancer patient.

Mentha pulegium L. is one of the Mentha (Labiatae) species that is grown in many different parts of the earth including northern Iran. The total ethanolic extract of the aerial parts of this plant is used in this work to investigate on the possible cytotoxicity on two different cell lines. Human lung carcinoma cells (A549) and human breast cancer (MCF7), have been used to show the clonogenic count of cells, as well as their cell cycle phases progression pattern after the exposure to the different concentrations of this extract. The cytotoxicity of the total extract of Mentha pulegium L. is 59 ± 1.73 µg/ml for the MCF7, and 48.86 ± 2.5 µg/ml for the A549 cell lines, respectively. Flow cytometry analysis have shown two different adventures for these cell lines after 24 hours exposure to this extract. While the MCF7 cells presented a dose-dependent arrest at the G2/M phase of the cell cycle, the A549 cells are more intended to accumulate in the so-called G0 resting phase after the exposure to this extract. These promising initial results might bring hope for the introduction of a tissue discriminating anticancer agent in future.