ririn astyka

One of the plants that potential to develop as an anticancer agent is Duku leaf (Lansium domesticum Corr.). From previous studies, Duku leaf extract had cytotoxic activity against several cancerous cell lines. The aim of this study was to determine the cytotoxic activity of crude and purified extracts of Duku leaf against MCF-7 and HTB-183 cell lines. The crude extract of Duku leaf was obtained using maceration in ethanol absolute. The vacuum liquid chromatography with a gradient mobile phase was performed to obtain the purified extracts. The crude and purified extracts were observed to inhibit MCF-7 and HTB-183 cells using 3-(4,5- dimethyl thiazol 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The antioxidant properties of samples were determined using radical DPPH scavenging activity. The data was collected and analyzed to provide the inhibitory concentration 50 (IC50) and Pearson’s correlation was analyzed to describe the correlation between cytotoxic and antioxidant properties. Five purified extracts were obtained and tested against MCF-7 and HTB-183. The purified extract D has stronger anticancer and antioxidant activities than other samples with IC50 of 56.26 ± 3.11 µg/mL, 70.94 ± 2.92 µg/mL,  and 53.65 ± 1.55 µg/mL, respectively (p < 0.05). We confirmed that cytotoxic activity has a strong correlation with antioxidant properties. Furthermore, the active compounds of purified extract D needed to be investigated and tested against MCF-7 and HTB-183 to explore the possible anticancer mechanisms.

The current investigation examined the potential antibacterial and antihyperlipidemic properties of ethanolic extract derived from the leaves of Garcinia atroviridis (EGA). The phytochemical contents of EGA were analysed through Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and Gas Chromatography-Mass Spectrum (GCMS). The antioxidant properties of EGA were carried using DPPH, reducing power, and radical OH scavenging methods, whereas antibacterial activity of EGA was conducted against 4 pathogenic bacteria using agar diffusion method. To test antihyperlipidemic action, High Fat Diet (HFD)-induced hyperlipidaemic rats were given EGA at 50, 100, or 200 mg/kg orally for five weeks. Body weight changes, liver weight, serum lipid profile, and liver histology were assessed. The EGA contains phenols and flavonoids by 23.53±0.27 mg GAE/g and 20.48±0.39 mg QE/g, respectively. Around 15 compounds were identified where hexanoic acid (30.55%), 9,12,15-octadecatrienoic acid (27.51%), and octadecanoic acid (15.49%) were compounds with the largest number. The EGA has antioxidant activity significantly different with ascorbic acid increasing %DPPH scavenging, %OH scavenging, and reducing power scavenging. The Minimum Inhibitory Concentration (MIC) was obtained at a concentration 10% with a clear zone diameter, while the Minimum Bactericidal Concentration (MBC) was obtained at different concentrations. This investigation was founded that EGA decreased biochemicals levels and causes liver tissue changes. Our studies demonstrate the EGA is antioxidant, antimicrobial, and antihyperlipidemic.