robab nazarpour

Training is essential for learning and clinical training is one of the fundamental parts of community health system. Although the professors have the central role of training in hospitals, the residents have an important role in training. The aim of this study was to determine the anesthesia student's attitudes to the role of anesthesia residents about clinical training.
In this cross - sectional study, a researcher-made questionnaire containing two main sections was used and its validity and reliability were confirmed, too. The first section included the questions related to the attitudes of anesthesia students toward the role of anesthesia residents and the second part was related to the comments of anesthesia residents to the anesthesia students. Anesthesia students and residents determined their attitudes by these alternatives such as strongly agree, agree, neutral, disagree and strongly disagree. The questionnaire was distributed among 100 anesthesia students of the third and higher semesters in continuous and non-continuous undergraduate.
FINDINGS: In total, 81 students who completely filled out the questionnaires were entered the study. Students believed that 33.3% of the residents could be successful in compensating the existing education gap, desirably. Also, 48.1% of students stated that the educational affaires were performed better by the residents than the related professors so that the majority of residents, in this regard, had been agreed or neutral. Among them, 32.1% of students agreed on the idea that training only by residents was not better than the current situation (by clinical instructor) and argued that the residents besides instructors improve their practical and clinical activities in addition to the increase of scientific knowledge.
According to the findings of this study, students had a desirable attitude toward the role of anesthesia residents. Anesthesia residents considerably help the students in the field of clinical training but this help requires the educational program and increases the motivation of high-year residents in clinical training.

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers، were measured using trypan blue dye exclusion method just before freezing with different concentrations of DMSO (10، 15، and 20%) and FBS (40 or 70%) at two different temperatures (either 4oC or 25oC). Then after 2 weeks the cells were thawed and the viability was measured again. Also the PBMCs response to PHA was measured after 48 h using MTT assay. The effects of the different variables were calculated and compared among the groups. A total of 192 PBMCs cryotubes made from blood samples of 16 volunteers were tested. The viability of the cells obtained by the two centrifugation procedure was the same (both more than 99%). The concentration of the FBS (40 vs 70%) did not show to have significant effects on either cells viability or response to PHA. On the other hand 20% DMSO concentration and freezing temperature at 25oC decreased both cells. Based on the obtained results، it is recommended to centrifuge the PBMCs under higher revolt speed at shorter time (700 g for 20 minutes) and decrease the FBS concentration to 40%. The DMSO concentration should be kept at 10-15% and the freezing medium be cooled down to 4oC.