roghayeh ghorbani

Breast cancer is the most common malignancy in the female population and is the leading cause of death. Surgery, chemotherapy, radiotherapy, and monoclonal antibody (trastuzumab) therapy are common and standard treatments for this cancer. However, there are significant limitations in the treatment of this disease by using regular methods. Given the role of transcription factors (TFs) in gene expression, differentiation therapy, and many cancer signaling pathways, in the present study, the effect of combined treatment of trastuzumab (Herceptin) and decoy oligodeoxynucleotides to target the STAT3 transcription factor on inhibition of HER2 positive SK-BR-3 cell line growth was investigated.

At first, different concentrations of Trastuzumab were prepared from 0.1 to 100 μg/mL. In this way, the decoy and scramble oligodeoxynucleotides were designed and synthesized to target the STAT3 transcription factor. The transfection efficiency and combined effects of trastuzumab with decoy oligos on cell viability, apoptosis, and cell migration inhibition were evaluated.

The results showed that oligodeoxynucleotides labeled with Cy3 fluorophore (300 nM) enter the cells with high efficiency (81.7±6.9%). Combined treatment of cells by trastuzumab (10 μg/mL) along with decoy oligodeoxynucleotides (100 nM) led to a decrease in cell viability (58.86±2.71%), increased apoptosis (24.54±4.83%) and inhibited cell migration (45.95±4.21%).

The results from this study showed the anticancer effects of combination therapy using trastuzumab with decoy oligodeoxynucleotides to target the STAT3 transcription factor in SK-BR-3 cells. Therefore, it seems that this strategy can be used as an adjunct to common treatments for breast cancer.

The aim of the study is to explain the mediating role of the moral virtue of patience in the relationship between successful intelligence and wisdom. The sudsy method is correlational in which structural equation model is used. The 300 undergraduate students from Shiraz University randomly selected by using a clusters such scales like websiters’ self-assessment of wisdom, successful intelligence of Sternberg and Gregorlenko (2002) and the patience of Khorrama’i and colleagues. The results of structural equation analysis show that the data are in line with the model and confirm that the direct effect of successful intelligence on wisdom and on the moral virtue of patience is (β = 0.057, β = 0.41, and p = 0.001), as well as moral virtue Patience on wisdom id (β = 0.16 and p = 0.001). Also, the model shows that the moral virtue of patience acts as intermediary between successful intelligence and wisdom (β = 0.06 and p = 0.001) respectively. These results reveal that cognitive variables such as successful intelligence along with and through the moral virtue of patience, are necessary to achieve wisdom. It is suggested that the teaching of successful intelligence and moral virtues such as patience be taken into account in order to strengthen and develop wisdom in individuals.

Staphylococcus aureus is one of the important bacteria causing nosocomial infections. Nowdays, because of overuse of antibiotics, resistance is increasing and this problem has caused anxiety all over the world. The aim of this study was to evaluation of antibiotic resistance pattern in S.aureus isolates from urinary tract infections in Shahid Beheshti educational and therapeutic center in Maragheh city.

In this cross sectional study, 100 S. aureus isolates were collected from patients suffering from urinary tract infections. After doing the biochemical tests for diagnosis of S. aureus, these isolates were identified as S.aureus with proliferation of thermonuclease species-specific gene (nuc) by polymerase chain reaction (PCR) method. Kirby-Bauer method was used for investigation of antibiotic resistance patterns in S. aureus isolates.

No significant difference in genotype frequencies was observed between the T2DM patients and normoglycemic controls. The rs7903146 (C/T) polymorphism odds ratios for CC and TC genotypes were 1.75 (95% CI, 0.63 to - 4.88; p=0.28) and 0.94 (95% CI, 0.49 to - 1.79; p = 0.84) compared with the TT genotype, respectively. The rs46522 (C/T) polymorphism odds ratios for TT and TC genotypes were 1.41 (95% CI, 0.58 to - 3.42; p = 1.41) and 1.22 (95% CI, 0.64 to - 2.35; p = 1.22) compared with the CC genotype, respectively.

Results of current study showed high antibiotic resistance in nosocomial S. aureus isolates. Therefore, antibiotics consumption should be avoided without proper prescription and unnecessary usage of routine antibiotics for showed be prevented.

Planting data and soil moisture retention are the most important factors influencing the increase crop yield. In order to evaluate the effect of planting dates and rice residue application (mulch) on growth and yield of cranberry bean, a factorial experiment arranged in a complete blocks design with three replications was conducted in Koochesfehan (Rasht, Iran). The factors were planting dates 3 levels (23 April, 30 April and 7 May) and mulch application with 2 levels (with mulch, without mulch). Results indicated that planting dates had significant effect on parameters, exactly number of green pods had significant effect on parameters, exactly number of green seeds, number of flowers, biological and productivity score. Interaction between planting dates and mulch had significant effect on number of dry seeds, number of root nodules, dry seed yield, biological yield and productivity score. Comparison of means indicated that first planting date had the highest effect on parameters; number of green seeds (29.79), number of dry seeds (30.03) and number of flowers (16) per plant, dry pod yield (1139.58 Kg/ha) and harvest index (47.93%). Mulch application had the highest effect on parameters; number of green pod per plant (8.33), pod yield (1085.28 Kg/ha) and harvest index (42.68%). Overall the first planting date (23 April) and application of mulch had higher effect on dry seed yield and productivity score of cranberry bean.

Road transportation of chemicals due to occurring various accidents and their hazardous consequences has high importance. The aim of this study was to determine safe distance in the accidents of road transportation of toxic chemicals in order to use in emergency response planning.
In this study, firstly, transportable chemicals -by road- were studied and ammonia, chlorine, 1,3-butadiene, benzene and toluene were selected for further study and modeling of toxic materials releases. Next, chemical exposure index (CEI) was determined and hazard ranking was done. Finally, modeling of toxic substances releases was done using ALOHA and PHAST softwares.
The results showed that, using the CEI index, ammonia, chlorine and 1,3-butadiene with exposure indexes of 597, 548 and 284, respectively, have high importance and need further investigation. In the final assessment that was done according to ERPG-3 concentration, using ALOHA software, ammonia with 2800 meters, and using PHAST software, chlorine with 3004 meters, have the highest hazard distances.
Assessment of the results showed that the used softwares and the CEI index dont have the capability to provide correct results at low concentrations (ERPG-1 & 2). Due to difference of the results using PHAST and ALOHA softwares, and CEI index, it is recommended to use the highest hazard distance-obtained by ERPG-3-for emergency response planning, without paying attention to type of the material and type of the softwares.

In recent years، more attention is paid to the more subtle aspects of school life such as students'' well - being. Therefore، knowing personal and social factors related to well - being is necessary. The aim of this research is to study the relation of self- regulation and communal mastery to psychological well - being. The research is correlational and the sample consisted of 300 (130 male، 170 female) students from Shiraz schools were chosen by multistage random cluster sampling. They completed self - regulation (Bouffard et al.، 1995) communal mastery (Hobfoll، 2000) and psychological well-being (Ryff، 1989) Questionnaires. For data analysis، Pearson correlations and stepwise regression analysis were used. The results indicated a significant positive relationship between the dimensions of self - regulation (metacognition، cognition & motivation) and communal mastery to psychological well-being. Also، the results showed that metacognition، motivation and communal mastery could explain 26% of psychological well-being variations. In sum، these results showed that teachers must remind students the importance of personal efforts and proper help - seeking.