فهرست مطالب s .dostdari

Brucellosis is one of the important zoonotic diseases, that causes fever in human and abortion in animals. Prevention of Brucellosis in humans is based on the vaccination of animals. Vaccination is the best way to prevent brucellosis in livestock. Rev.1 vaccine is one of the most effective vaccines to prevent and control brucellosis in sheep and goat. Since one of the most important topics in biological products  make ensure the quality of the product at all stages, including during storage and consumption.Also,according to the field conditions and the vaccine injection process,evaluation of Rev.1 vaccine stability after solubilization lyophilized vaccine is necessary. the present study was conducted to evaluation stability of Rev.1 vaccine after solublizathion. To do this, samples were collected randomly from three consecutive batches of Rev.1 vaccine. Serial dilution were prepared with physiological saline, and the live mass of the Rev.1 vaccine was obtained. After that dissolved vaccine was kept in different temperature conditions  at 4°C, 25°C and 37°C, Afterword four, eight, 12, 18 and 24 hours after solublizing the vaccine, the live mass of the  Rev.1 vaccine was analyzed. The results showed that Rev.1 vaccine after being solublized was congurous with OIE 2019 criteria when maintained 24 hours at 4°C, 18 hours at 25° C and 12 hours at 37° C. Therefore It is recommended to use the vaccine after solublizing procsee as soon as possible to prevent the reduction in the titer of Rev.1 vaccine and loose of time.