فهرست مطالب s. rouhani

Today, it is important to pay attention to the reduction of toxic effluents and pollution caused by industrial effluents. The textile industry and dyeing procedure is one of the most important industries in the world, and its effluent causes environmental pollution. Reducing the use of chemical compounds in the dyeing process can reduce these adverse effects. In this article, the aim is to investigate the use of the natural colorant of Madder in the presence of a mixture of two natural tannins-rich mordant (Myrobalan and Walnut husk). For the application of mordant, the pre-mordanting method is used and their performance is compared with Alum. A mixture of two natural mordant with different percentages, the total of which will be 10 %, was used. The changes in the application of the mordant and the natural dye of the yarns are evaluated using the FTIR test. The removal of the peak related to C-N bonding indicates the proper interaction between the mordant, the dye and the yarns. The results illustrated that the use of mordant and increasing the dye concentration increases the color strength. The results showed that the best percentage of mordant mixing is WH:YM=7.5 %:2.5 %. ISO standards were used to check the fastness properties of dyed yarns and moderate to good results were obtained.

In this paper, four indoline-based organic dyes were introduced and studied as photosensitizers in the photovoltaic devices. The starting material for preparing organic dyes by standard reactions is carbazole and phenothiazine. The dyes' status changes are assessed by spectrophotometric measurements of the organic photosensitizers in acetonitrile and on a TiO2 and rGO/TiO2 substrate. The maximum absorption wavelength for Dyes 1-4 in acetonitrile, TiO2  films, and rGO/TiO2 was investigated. Finally, the proposed dyes used as a photosensitizer in a dye solar cell structure in the presence of rGO/TiO2 and their photovoltaic properties were investigated.

A novel "turn-on" nano chemosensor for Hg2+ was developed based on  CoFe2O4@SiO2 nanocomposite. Cobalt nano ferrite particles were decorated with 1,8-naphthalimide dye conjugated with rhodamine dye.  It was characterized using X-ray powder diffraction (XRD), transmission electron microscopy (TEM), FT-IR techniques. A fluorescence enhancement was observed upon binding Hg2+ to two core chromogenic dyes. No significant change in the fluorescence intensity was observed in the presence of other metal ions. The results showed that the functionalized nanocomposite CoFe2O4@SiO2/NR exhibited selective 'turn-on' fluorescent enhancements with Hg2+. Also, the company of magnetic CoFe2O4@SiO2 nanocomposite (with a wide range of applications such as biomedicine, magnetic fluids, magnetic energy storage) and catalysis facilitates the magnetic separation of the Hg (II) from the solution. Nano chemosensor exhibits high selectivity, high sensitivity and fast response to trace mercury ions. Designed nanosensor successfully applied for Hg2+determination at real samples with a linear range of 0.04-0.76 μM of Hg2+ ions. It was successfully applied for the determination of mercury ion in real samples of tap water. It seems that the presence sensor is a suitable candidate for the detection of trace mercury ion in biomedical samples like a human serum.  Also, it is fast and easy control for monitoring of water toxicity of pollutant industries.

Strongyloides stercoralis is prevalent in tropical and subtropical regions of the world. S. stercoralis is the only nematodes with the ability to multiply in its host's body via autoinfection transmission. Larvae detection in faeces is difficult partly because of low egg production and irregular larvae excretion in faeces. Serologic tests (ELISA, IFA) are also diagnostic, however, S. stercoralis antigens are not available as a diagnostic tool. In the present study, we analyzed filariform larva (L3) proteins of S. stercoralis by the immuno blot technique.

Stool samples were examined by direct smear, formalin-ether and agar plate method to identify the patients. Sera were stored at 20°С. Filariform larvae were obtained by agar plate culture, which was incubated for 6-7days at 25ºC, then frozen at – 70°С. Finally, larvae were suspended at a concentration level of 12000 in 250l PBS, containing protease inhibitors and then sonicated. Protein level was measured by Bradford method. Proteins of S. stercoralis filariform larvae were separated by SDS-PAGE, blotted onto nitrocellulose paper. Western blot analysis of these antigens was achieved using infected human sera (0.1, 0.01, 0.001 dilution) with strongyloidiasis, toxocariasis, hydatidosis, amoebiasis and normal human serum as control.

Four immunodominant proteins (23, 28, 30, 41 kDa) were recognized with strongyloidiasis sera in 0.1diluted serum. None of the proteins reacted to normal human and amebiasis serum, but some showed reaction with serum of hydatidosis and toxocariosis. Having increased the level of serum dilution, only 41 kDa protein was recognized by strongyloidiasis sera. Other sera did not show any reaction to the parasite’s proteins. Therefore, the 41 kDa protein presents as most important immunodaminant protein in this study.

The identification of immunodominant proteins, which have adapted themselves to the physiological and genetically conditions of the host is an appropriate diagnostic approach. Indeed, recombinant protein, such as 41 kDa, could enhance the sensitivity and specificity of serologic tests.