فهرست مطالب saeedeh amani

Pulegone (PGN) is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the (R)-() PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptorα ( ERα) and ERβ receptors expressions.
In this experimental study, mature albino mice were divided into experimental (received 25 mg/kg, 50 mg/kg and 100 mg/kg PGN, orally for 35 days) and control (received 2% solution of Tween 80 as a PGN solvent, orally) groups. The mRNA levels of Erα, Erβ, p53, Bcl-2, and cytochrome p450 (Cyp19)
as well as ovarian angiogenesis were analyzed through reverse transcription polymerase chain reaction and immunohistochemical techniques, respectively. Moreover, apoptosis of follicular cells, serum estrogen and progesterone levels and mRNA damage were investigated via using terminal transferase and biotin-16-dUTP staining, electrochemilunescence and fluorescent microscopy methods, respectively.
The PGN reduced Erα, Erβ and Cyp19 expression at 50 mg/kg and 100 mg/kg doses, while significantly elevating p53 and reducing Bcl-2 expression. Finally, PGN impaired ovarian angiogenesis, increased apoptosis, elevated follicular atresia and reduced serum levels of estrogen and progesterone.
Chronic exposure to PGN (50 mg/kg and 100 mg/kg), severely affects ovarian aromatization, protooncogenes mRNA levels and expression of ERs.

Peripheral arterial disease is the result of obstruction of blood circulation. Mast cells and platelets via induction of angiogenesis may cause the enhancement of amelioration local ischemia. The aim of this study was help to find an appropriate approach to recovery of ischemic condition in the restricted femoral artery localization.
30 male white Wistar rats were divided into 6 ischemic experimental groups (n=5), randomly: Ischemia control group: the femoral artery was transected‚ Phosphate buffer control solution (PBS) group: the transected location was immersed with PBS, Chitosan control group (CHIT): the transected location was immersed with chitosan solution, Mast cell transplanted experimental group (CHIT/Mast cells): the transected location was immersed with chitosan and mast cells (1× 106). Platelets transplanted experimental group (CHIT/plat): the transected location was immersed with chitosan and platelets (13×106). Platelets and mast cells transplanted experimental group (CHIT/plat/Mast cells): the transected location was immersed with chitosan, 13×106 platelets and mast cells (1×106).
Analyses of capillary density and histomorphometrical analysis were performed on day 7 after surgery. Mean number of blood vessels in CHIT/Mast cells group indicated a significant difference compared to other experimental groups (PMast cells using in tissue engineering could offer a new approach for therapeutic angiogenesis which is able to provide improved neovascularization in cases of peripheral arterial diseases.