sajjad yaghoubi

Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA, and iceA2 genes in H. pylori strains isolated from gastroduodenal patients, who referred to Shariati hospital in Tehran, Iran.

Gastric biopsy specimens were collected during endoscopy from patients, who referred to the Shariati hospital in Tehran, Iran during January and November 2015. After isolation of H. pylori from the biopsy culture, genomic DNA was extracted and subsequently used to identify H. pylori and virulence genes using specific primers.

The isolation rate of H. pylori strains was 65.7% (169/257). The frequency of cagA, vacA, cagE, iceA1, oipA, and iceA2 was 143 (% 84.6), 169 (100%), 131 (77.5%), 97 (57.3%), 89 (52.6%), and 72 (42.6%), respectively.

In this study, a significant difference was observed between investigated genes and strains isolated from PUD and GC patients (p

RFLP-IS6110 standard technique to genotyping M. tuberculosis.The aims of this study were to identify the genetic diversity of M. tuberculosis population in Markazi province and to recognize the mode of disease transmission in this region. The RFLP results of 42 isolates of M. tuberculosis deposited in the Mycobacterial Centre from Markazi province were analyzed. DNAs isolated from these isolates were enzyme digested with Pvu II, and hybridized with a PCR amplified DIG-labeled IS6110 probe. The isolates were classified into four groups, based on the copy numbers, as follows: (1) lacking IS6110 element; (2) low copy numbers (1-2); (3) intermediate copy numbers (3-5); and (4) high copy number (6-17). Copy numbers higher than 17 however were not observed in any of the isolates studied, 72 percent of the isolates showed high copy numbers of IS6110, 13 percent intermediate copy numbers, 10 percent low copy numbers, whereas 5 percent isolates lacked IS6110 element. IS6110 DNA fingerprinting assisted us to find epidemiological links between some TB cases, and this technique estimates from reactivation of latent infection transmission of the disease in Markazi province. The low rate of clustering indicates that tuberculosis among studied population is resulted mainly from reactivation of latent infection in this region.