فهرست مطالب saman saadat

The Mycoplasma Hominis bacterium lives on in the female reproductive system. Recent studies have shown that the presence of this bacterium is associated with disorders such as vaginosis, infertility, abortion and preterm labor. This study was performed with aim to determine the frequency of Mycoplasma hominis in women referred to Infertility Clinic of Hamadan Fatemieh Hospital.
This epidemiologic-descriptive study was performed on 234 patients referred to the infertility clinic of Hamadan Fatemieh Hospital in 2016. Screening was performed using swabs from the endocervix of 234 female patients who had at least one of the symptoms of vaginosis, infertility, abortion and preterm labor. The culture medium was removed by filtration and the PCR molecular technique was used to trace the 16s rRNA gene as two methods for detection of bacteria. Data was analyzed by SPSS software and McNemar's test. P The frequency of Mycoplasma hominis in both methods was 13.7%. The correlation coefficient between culture and PCR methods was relatively desirable for detection of this bacterium (k=0.5). 41 patients (24.4%) with history of at least one abortion and 148 (14.2%) with vaginosis were diagnosed as positive Mycoplasma agar. The highest prevalence of Mycoplasma hominis was observed in the age range of 30-39 years.
Although the PCR molecular technique has high sensitivity to diagnose Mycoplasma hominis, but the modified culture method has an acceptable sensitivity to detect this bacterium.

Because of clinical importance of Mycoplasma hominis especially in gynecology and difficulties related to diagnosis of this organism in our laboratories, it is essential to more accurately evaluation of the menti oned bacteria frequency in Iran. In this study the technique of culture and formulation of media have been designed in order to increasing the probability of bacterial isolation from the patient’s clinical specimens favorably. Moreover, frequency of Mycopl asma hominis in the studied population and also relationship between presence of organism and clinical signs has been evaluated. This research was a descriptive epidemiological study which wasperformed on 226 patients referred to women clinic of Qazvin Kowsar hospital in 2012 - 13. The specimen was endo - cervical mucosa which was collected by dacron swabs. The swabs were initially placed in broth culture media and then maximum after 24 hours were transferred on agar plates. In this study observation of fried egg shaped colonies on the surface of agar culture media was considered as a positive culture and color change of broth media alone was reported as negative. Among the 226 specimens obtained form patient’s cervical m ucosa, 30 cases (13.2%) were positive for Mycoplasma hominis based on colony formation on agar media. Frequency of Mycoplasma hominis in Qazvin is considerable and more investigations are necessary. The technique of culture and formulation of medium used in this study can improve sensitivity of the culture method.