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Cortisol is one of the most significant factors contributing to controlling stress. Additionally, another well-known mechanism for protecting cells against various stresses and linked to cortisol is the reaction to heat shock, which triggers the production of heat shock proteins (HSP). By strengthening sturgeon fish's defense system against environmental stress, such as temperature stress, their stocks can be preserved. In this study, sterlet sturgeon (Acipenser ruthenus) liver tissue cells were extracted and cultured in flasks. Nopal Endurance (Pro-Tex®), amygdalin, and pyrazole derivatives (SZ) were used as HSP inducers (HSPi). In order to determine the viability and optimal dose, MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide) assay was performed. The activity of cortisol and biochemical enzymes was analyzed in liver cells under temperature stress (18, 22, and 26 °C) in the presence and absence of HSPi compounds. MTT assay showed that cells receiving HSPi compounds increased survival. They could increase cortisol levels alone or in combination with stress. Amygdalin reduced biochemical enzyme activity.Discussion and Escape from apoptosis under stress conditions is a basic strategy for cell survival. Considering the role of cortisol and its relationship with HSP in environmental stress conditions, it can be concluded that treating cells with HSPi compounds can make cells resistant to stress and the harmful effects caused by stress reverse and ultimately increase survival.

During the uncontrolled development of cells in the body, a subset of neoplasms or tumors is formed, the abnormal proliferation of these cells leads to the formation of a mass and eventually cancer. This mass can spread throughout the body. Thus, inhibiting the abnormal growth of cancer cells will have a significant effect on preventing the spread of cancerous tumors and improving the disease. Therefore, in the present study, a new sulfonamide derivative was designed and synthesized (HB20) and its anti-cancer effects on human breast cancer cell line (MCF-7) were investigated.

For the synthesis of a sulfonamide derivative (HB20), dcriptiazonium salt was first made using a sulfamethoxazole base compound and then combined with a pyrimidine coupling agent. Concentrations of a new synthetic compound (HB20) against Cells (MCF-7) were used. MTT assay was also performed to measure survival and cell proliferation.

The synthesized compound structure was confirmed by spectral analysis, such as FT- IR, and NMR. Also, Survival in MCF-7 cells treated with a synthetic compound (HB20) was significantly reduced compared to the control group (untreated). HB20 inhibits the proliferation of MCF-7 cancer cells with an IC50 value of 75/23 μg/ml.

The new sulfonamide derivative (HB20) has the potential to inhibit proliferation and anti-cancer properties in the cell line (MCF-7).

To date, various derivatives of thiazolidinone in a variety of cell lines have been investigated. The present study aimed to evaluate the toxicity and inhibitory effects of a thiazolidinone derivative called 5-(2,4-bis- 4-ethoxy-phenyl azo)-3-hydroxy-benzylidine)-2,4-thiazolidinone (TZD-OCH2CH3) on the expression of NF-кB in LPS-induced RAW264.7 macrophage cell lines. Different concentrations of the MTT assay (0-120 μg/mL) were performed to estimate the biological rate of the cells. The half-maximal inhibitory concentration (IC50) of TZD-OCH2CH3-treated RAW264.7 cells was found to be 115 μg/mL. To determine the inhibitory effect of the synthesized compound on the expression changes of NF-кB, the RAW264.7 cells were initially induced with LPS and then treated by 15, 30 and 60 μg/mL of TZD-OCH2CH3. Real- time PCR results confirmed a strong inhibitory effect of TZD-OCH2CH3 on the expression of NF- кB in LPS-induced RAW264.7 cells (IC50 = 48 μg/mL). Overall, these findings suggested that the derivative TZD- OCH2CH3 had a significant anti-inflammatory ef