seyed amirhosein hoseini

Up to now, several attenuated measles virus vaccine strains derived from the Edmonston B vaccine consisting of Schwarz/Moraten, Zagreb, and AIK-C have been introduced for the rescue of their relative viruses through reverse genetics. In most studies, the measles virus rescue was done by supplying a cell line that expresses T7 RNA polymerase and measles virus N and P proteins as accessory proteins. The present study aimed to evaluate the rescue efficiency of the recombinant measles virus AIK-C vaccine strain by using a tricistronic expression plasmid. In this study, the rescue of the recombinant measles virus AIK-C vaccine strain was performed by co-transfection of three plasmids, including the cloned antigenomic cDNA of measles virus, a tricistronic expression plasmid that contained T7 RNA polymerase and measles virus N and P genes, and measles virus L polymerase expression plasmid. To increase the rescue efficiency, the transfected HEK-293 cells were co-cultured with Vero cells. As a result, the use of tricistronic expression plasmid that concomitantly encoded three necessary genes for the rescue of the measles virus led to the viral cytopathic effect with high efficacy five days post-transfection. Finally, the co-culture of transfected HEK-293 cells with Vero cells showed a relatively fast induction of viral cytopathic effect.

Nowadays, various factors complicate the decision making process and the need to make decisions that consider all the factors in question, they are more than ever before. The internal and external researchers, have shown interest in the multi-criteria decision making. In our study, the method includes a multi-criteria decision making method such as Analytical Hierarchy Process, Simple additive weighting and TOPSIS )Technique for order-prefrence by similarity to ideal solution( to solve the selection problem of building efficient systems we have used. Systems that have been evaluated in this issue include: Lightweight Steel Frames, Insulating concrete formwork, 3D-PANEL and Prefabricated reinforced concrete systems. Required data using the questionnaire sample (n = 150) were examined. Questionnaires among the mass of experts, academic institutions and conferences were distributed. According to the research results, using different methods of decision making, the results will yield fairly similar, so that all three methods, Prefabricated reinforced concrete systems in the first place, and the Lightweight Steel Frames system was in the Second place. The Prefabricated reinforced concrete systems, in terms of administrative and economic criterias, in the first place, and the system Lightweight Steel Frames, ranked first in terms of environmental criteria accounted for.