فهرست مطالب seyyedeh mahdokht maddah

Lung cancer is one of the most common types of cancer in the world and Iran. Herbs are a source of a variety of antioxidant compounds that can be used to produce anti-cancer drugs. In the present study, the effect of cytotoxicity of essential oil and hydroalcoholic extract of Juniperus excelsa on A549 lung cancer cells was investigated. The branches of male rootstocks of Aras plant were collected from Chehel Cheshmeh in Firoozkooh and Sirachal in Karaj and the essential oil and extract were prepared by Clevenger and soaking methods, respectively. The degree of cytotoxicity, different concentrations of essential oil and extract of the two regions, on A549 cells in three times of 24, 48, and 72 hours Was evaluated using MTT method. Flow cytometry was used to evaluate cell death and cell cycle in cells treated for 72 hours with 10 µg/ml of essential oil and extract of two regions. The results of cytotoxicity showed that the lethal power of extracts was significantly higher than essential oils, but there was no significant difference between different regions (P <0.01). Also, a further decrease in the number of viable cells and an increase in cell entry into the Sub G1 phase, resulting in an increase in apoptosis treated with male root extract, was proven. Thus, the extract of the branches of this plant has anti-cancer properties by stopping the cell cycle in the G2 / M stage.

Cervical cancer is the second most common cancer among women. Interleukin-8 (IL-8) is a member of the chemokine family and is involved in initiating inflammatory reactions. Previous studies have shown that polymorphisms in the IL-8 gene are associated with an increased risk of various types of cancer. This study was performed with aim to investigate the association between IL-8 -251A> T polymorphism (rs4073) with cervical cancer in the female population in Tehran using Tetra primer arms-PCR.

In this case-control study which was performed in 2018, a total of 100 blood samples were examined, of which 50 samples were related to women with cervical cancer and another 50 samples belonged to healthy women as a control group. After DNA extraction from all samples, the frequency of IL-8 -251A> T polymorphism (rs4073) was evaluated using Tetra primer arms-PCR technique. Data were analyzed by SPSS statistical software (version 21) and chi-square test. P<0.05 was considered statistically significant.

The frequency of TT, AT, AA genotypes was 42%, 50% and 8%, respectively in the patient group and 24%, 70% and 6%, respectively in the control group. The frequency of A and T alleles in the patients was 33% and 67% and in the healthy individuals was 41% and 59%, respectively. There was no significant difference between the control and patient groups in allelic distribution (P = 0.24) and genotype in all three codominant models (p=0.16), dominant (p=0.055) and recessive (P = 0.6).

There was no relationship between IL-8 gene T-251A polymorphism and cervical cancer in the population of women studied in Tehran.

Rhabdosciadium aucheri (Apiaceae) is a perennial herbaceous species, which is endemic to W, S and C Iran. In the present study aqueous, ethanolic and methanolic extracts were obtained from aerial parts of the specimens collected from Hamadan province, using both Maceration and Soxhlet methods. Due to the direct relationship between total phenolic and flavonoid contents and the plant antioxidant activity, the aim of the present investigation was the comparative study of total amount of each parameter extracted by aqueous and hydroalcoholic solvents. The results demonstrated that, the highest amount of phenolic and flavonoid compounds extracted by ethanolic solvents using Maceration technique were 37.28 ± 1.03 mg GAE/gr GW and 22.68 ± 0.63 mg QE/gr DW respectively. These results were significant at p < 0.05. Moreover, 72.17 ± 17.10 % was the highest free radical inhibition percentage for methanolic extracts. However, the differences were not considered as significant. The results also showed that using hydroalcoholic solvents are more powerful compared to aqueous solvents in the extraction of phenolic and flavonoid contents and consequently their antioxidant activity.