shahin masoudi

Two structural antigens, haemagglutinin (HA) and neuraminidase (NA), are attractive candidates for the development of a genetically engineered vaccine against influenza. Recombinant vaccines are produced by a simple and effective method, although expected to induce an immune response to a specific antigen, remain to be further improved for their high effectiveness. On the other hand, a potent and effective vaccine against influenza should be able to induce both humoral and cellular immune responses. In the present study, the NA gene, which is more stable than the HA one, was amplified by polymerase chain reaction (PCR), and the band appeared in the range of 1400 bp. Then cloned into a eukaryotic expression vector pFastBac HTA. The purity of the expressed NA protein was analyzed on SDS-PAGE electrophoresis. In the western blot, a band with a weight of about 41 kDa produced by the recombinant baculovirus containing NA is observed compared to the uninfected cells used as a negative control, and a recombinant neuraminidase is confirmed. Finally, neuraminidase (NA) gene clone can be used to make recombinant human influenza vaccine.

Infectious bronchitis is an extremely contagious viral disease and causes the major health problems of the poultry industry in most countries of the world. The incidence of continuous genetic and antigenic changes in infectious bronchitis viruses challenges the bird's immune responses. Therefore, a vaccine to prevent the disease in young chickens may effective when formulated with circulating serotype. In case, live infectious bronchitis 793B/08IR was developed in Razi Institute at laboratory scale. The potency of the vaccine was evaluated in commercial broiler chickens in controlled conditions. Two hundred and fifty one-day-old Ross-308 chickens were divided into the four treatment groups and one control group (n=50). The two treatment groups were received 793B/08IR vaccine and other groups received an imported one via eye drop method. The booster was administrated three weeks post vaccinations. Blood samples were collected from chickens in each group before vaccination and at intervals of two-week. The potency of 793B/08IR vaccine was evaluated using ELISA and serum neutralization assays and compared with an imported vaccine. The neutralizing index data and increase in serum antibody titer in treatment chicken groups indicated that live infectious bronchitis 793B/08IR has proper potency against the disease.

La bursite infectieuse (maladie de Gumboro) a été rapportée de partout dans le monde et limportance socio-économique de cette maladie est considérable au niveau international. Plusieurs types de vaccins commerciaux ont été introduits pour contrôler cette maladie. Ces vaccins sont produits par culture cellulaire ou dans des œufs embryonnés. Si les vaccins à base de cellules contre la maladie de Gumboro s’avèrent sans danger et efficaces, ils seront beaucoup plus abordables pour le traitement de la maladie. Les fibroblastes embryonnaires de poussin (CEF) sont les cellules les plus populaires pour la production du vaccin contre la Bursite infectieuse par culture cellulaire. Le procès de préparation et dutilisation des cellules CEF est très long et difficile. Dans cette études, l’attention est portée pour la première fois sur la référence Annex3 TRS 978, WHO, une cellule en suspension de moutons dorigine lymphoïde (H1 Cell Line) ayant montré une sensibilité au virus vaccinal de Gumboro. Cette cellule a passé avec succès tous les tests et a été adaptée à la réplication du virus de la Bursite infectieuse.

Infectious bronchitis is an acute، highly contagious، viral disease of poultry with worldwide distribution، and is continuously evolving through point mutation and recombination of their genome; subsequently the emergence of IBV variants complicates disease control. To investigate genetic characterization of new IBV variants isolated from commercial chicken flocks in Iran collected between 2009 and 2010. METHODES: The partial S1 gene of the spike protein، covering a hypervariable and constant regions، was amplified and sequenced using conventional RT-PCR. Phylogenetic analysis revealed four viruses designated as Razi-HKM891، Razi-HKM892، Razi-HKM893 and Razi-HKM894. Deduced amino acid sequence comparison with other IBV genotypes، published in the GenBank database، indicated that the isolates Razi-HKM891 and Razi-HKM894 were placed into the pathogenic 793/B serotype. However، the isolates Razi-HKM892 and Razi-HKM893 were different with previously described isolates in Iran. The Razi-HKM893 is closely related to recently published isolates from countries in Middle East and likely indigenous to Iran. These findings is essential for improving the disease control strategies and thus emphasize the importance of continuous surveillance of the disease and of sharing the information to the global scientific community، which would help to fill the epidemiological gaps in the regions and to validate the robustness of diagnostic screening.