shekofeh baghazadeh

Sperm motility has a basic role in male fertility. The aim of this study was to assess the changing trend of different types of sperm motility during the storage of normozoospermic men's semen samples in refrigerator (4-6 ˚C)
In this experimental study, 13 washed semen samples of normozoospermic men were kept in refrigerator (4-6 ˚C) for 12 days. At 0 (immediately after sampling as control group), 1st, 2nd, 5th, 7th, and 12th days, the proportions of fast progressive, slow progressive, non-progressive and immotile sperm cells were calculated.
Findings: The percentages of different kinds of motile spermatozoa decreased with a gentle slope (P This study suggests that after 12 days of keeping semen sample in refrigerator, no motile sperm can be found in culture medium and more motile spermatozoa can be retrieved up to first 48 hours.

Previous studies have revealed that high doses of Thymoquinone (TQ) is able to cease the movement of sperms in culture medium.The aim of this study was to investigate and evaluate whether Nigella sativa (NS) extract has similar effect on spermatozoa with TQ or not.
Ten washed semen samples were used for each concentration of NS (5 and 10 mg/ml). Two 0.5 mlaliquots of each sample were incubated with or without the considered dose as case and control samples. Total sperm motility was assessed after one hour. In addition, apoptosis of sperms receiving dose of 10 mg/ml were measured by Annexin-PI staining method followed by flow cytometry detection.
Findings: Both doses of NS extract substantially reduce the percentage of total motility, fast and slow progressive and non-progressive sperms. After administration of NS extract, the percentages of alive, apoptotic and necrotic sperms did not show significant change.
5 and 10 mg/ml doses of NS extract considerably diminish the percentage of motile spermatozoa in culture medium. Similarly, NS extract with dose of 10 mg/ml does not change the percentage of viable spermatozoa in culture medium.

Sperm motility is a highly complex molecular process which is the result of transverse waves exist along its flagellum. Thymoquinone (TQ) is the most abundant active component isolated from black seed (Nigella Sativa). It was revealed that in-vivo administration of thymoquinone could improve spermatogenesis and increase number and motility of spermatozoa. The aim of this study was to assess in-vitro effects of low doses of thymoquinone on motility and viability of human spermatozoa. Twenty semen samples of normozoospermic men were washed in modified Ham’s F10 medium containing albumin. 10 semen samples were used for each concentration of thymoquinone (5 and 10 μg/ml). Each sample was washed and two aliquots of it were incubated with or without considered dose of thymoquinone. Sperm motility and viability were assessed after two hours of incubation. Also, sperm motility was graded as fast- and slow-progressive, non-progressive and immotile. Both doses of thymoquinone increased the percentage of total motile and fast-progressive sperms. Administration of 10 μg/ml of thymoquinone increased the percentage of slow-progressive sperms while the dose of 5 μg/ml reduced it. The percentage of non-progressive and immotile sperms was decreased but the percentage of viable sperms was not changed after using thymoquinone. Low doses of thymoquinone can increase sperm motility in culture media.