shilla safaeian

In this research, some nutraceutical properties of Padina algae extract were identified and its nanoliposomes were prepared to achieve a sustained release of bioactive substance. According to GC-MS analysis, butanoic acid was the foremost substance and n-hexadecanoic acid was the second major chemical constituent in the extract. In addition, there are significant triterpenoids in the algal extract, which could be the possible reason for Acetylcholinesterase inhibitory activity, which has been recognized as a potential therapeutic strategy for the treatment of Alzheimer's disease. The formulation of algal extract-loaded nanoliposomes was optimized based on the variation of the wall-to-base ratio. The effect of lecithin in different ratios on the concentration of Padina alga extract mean size, polydispersity index, zeta potential, and encapsulation efficiency was evaluated. The results indicated that nanoliposomes loaded with algal extract showed a smaller size (318±3.05 to 59.95±0.19 nm), narrow size distribution, acceptable EE (15.7±0.92 to 52.8±0.52%), and good antioxidant activity (92 mg/mL). Morphological characteristics of the nanoliposomes using a transmission electron microscope reveal uniform particle with a spherical geometry. Moreover, the optimally formulated nanoliposomes show sustainable release of their contents into the simulated gastrointestinal fluids. These results can appear to the prospect of the possible use of this extract in the nano liposomal structure with a therapeutic approach.

Microencapsulation of bioactive compounds in lipid carriers, such as liposomes, in addition to improving stability during storage by increasing bioavailability and controlled release, increases the efficiency of these compounds in vivo. The studies conducted on Padina algae show the existence of a high level of phenolic and antimicrobial compounds. Also, this alga has a significant amount of polyphenols with antioxidant and anti-AChE (acetylcholinesterase) properties, which can be used as a supplement to improve neurological disorders. Therefore, the purpose of this research was to produce and investigate the structural properties of nanoliposomes containing Padina algae extract using the heating method. The particle size of nanoliposomes produced at varying levels of lecithin and loaded extract was obtained in the range between 318 and 60 nm. The resulting values ​​for the polydispersity index and zeta potential indicate the uniformity of the produced particles along with the high electrostatic repulsion between the particles. The ability to load liposome particles at the lowest level of wall substance and the highest concentration level of the extract reached 52.8±0.3% in this research. Evaluation of the morphological characteristics of the structure using a transmission electron microscope shows the formation of uniform particles with a spherical geometry. The results of this research show the ability to produce a liposome structure containing Padina algae extract with suitable structural properties. These results can improve the prospect of possible use of this extract with a therapeutic approach.

Sargassum and Padina are two genera of brown algae that are widely scattered in temperate regions. Sargassum species are categorized as tropical and sub-tropical brown seaweed which are valuable sources of bioactive compounds including dietary fibers, carotenoids, vitamins, and minerals. These brown algae demonstrate diverse biological activities such as antioxidant, antimicrobial, and anti‑Alzheimer, due to the presence of flavonoids, triterpenoids, flavonoids, sterols, polyphenols, and pheophytine. The genus Padina is scattered in many environmental conditions, mainly in the tropical marine waters, and belongs to the family Dictyotaceae. Some bioactive components isolated from Padina species have been demonstrated hypoglycemic, hypolipidemic, anti-obesity, hepatoprotective, cardioprotective, immunostimulatory, and antimicrobial activities., The aim of this study was to prepare an extract from two species of algae Padina and Sargsum by massaging and ultrasound assisted-methods  as well as analyzing their compounds and investigating the antioxidant, antimicrobial and enzymatic properties of the extracts. According to the obtained results, ultrasound assisted method was a suitable method for extraction. This extract can be used as a combination of antioxidant, antimicrobial, anti-Alzheimer's and nitrate reducing agent in food additives.

Chemical materials were supplied by Sigma-Aldrich GmbH (Sternheim, Germany). The algal species utilized in the current investigation; namely, Padina distromatic and Sargassum angustifolium were collected from the coastal region of Chabahar bandar, Sistan and Baluchistan Province, Iran. To eliminate all the impurities and extraneous materials, they were washed by using distilled water and then dried at ambient temperature (24-48 h) until the constant weight. Extraction by maceration was compared with the extraction using ultrasonic assisted method. Determination of chemical compounds was parformed using GC-MS device. Investigation of antioxidant properties and total polyphenol and flavonoid content were also performed. The degree of free radical scavenging was done according to DPPH method. Evaluation of antimicrobial effect of algae extracts were the main challenges in our research. S. aureus ATCC25923, Listeria innocua ATCC 33090, E. coli ATCC 25922 and S. typhi ATCC 6539 were used for antimicrobial test. Determination of minimum growth inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were performed using wells in agar.The enzymatic activity was also determined. In this study, the activity of acetylcholinesterase was investigated using the method introduced by Ellman et al. (1961) and also the activity of nitric oxidase was determined using a kit protocol (Nvand-Iran). Factorial experiment in the form of a completely randomized design was used to analyze the data. Duncan's multiple range test was used to determine the difference between the means at the 95% confidence level, and SAS software version 2.9 will used for statistical analysis.

The current study investigated the antioxidant, antibacterial, anti-inflammatory, and anti-Alzheimer’s attributes of two brown algae namely Padina distromatic and Sargassum angustifolium which were collected from the coastal region of Chabahar Bandar, Iran. The results clearly indicated that the type of algae and extraction techniques used in this investigation highly affected phytochemical compositions, antioxidant, AChE inhibitory, scavenging, and antimicrobial activities. Considering both extraction yield and phytochemical components, extraction by ultrasound assisted method provided better results. Among all algal extracts, UPE presented the highest AChE inhibitory activity, and antibacterial activity and USE presented the highest antioxidant activities, total phenols and flavonoids, reflecting the presence of various bioactive components. The extracts of two various seaweeds utilized in the current study highlighted considerable inhibitory action against four pathogenic bacteria. According to the observations of the antibacterial assay, S. aureus was the most sensitive microorganism, while L. innocua was revealed as the most resistant bacteria to the extracts of P. distromatic and S. angustifolium. Further, the chemical components responsible for the antioxidant, AChE inhibitory, and antibacterial power were confirmed by GC-MS analysis. The findings of the current investigation confirmed the potential of the health benefits and therapeutic effect of brown marine algae. Thus, the extract of P. distromatic and S. latifolium could be an effective supplement to be incorporated into the products’ formulation in the food and pharmaceutical industries as well as in medication to alleviate several disorders such as Alzheimer.

The use of medicinal plants or the components extracted from them in different sectors of the food industry has received special attention. Therefore, in the present research, linseed oil was used in two forms, free and microcoated with alginate, in the formulation of cocoa cream. The aim of this research was to produce an functional chocolate ganache containing natural preservatives. The extraction efficiency of linseed oil was 27.73 %. The main component of linseed oil was α-linolenic acid (47.1%) and the least fatty acid detected in it was myristoleic acid (0.06%). The results showed that both evaluated oils had considerable antibacterial effects against tested microorganisms (S. Typhi, E.coli, S. aureus, A. niger, and C. albicans) and gram-negative bacteria were more resistant to linseed oil than gram-positive ones. In this regard. Black seed oil showed higher antibacterial activity and both linseed and black seed oil microcapsules had lower antibacterial effects than their free form. The sample containing 3% encapsulated linseed oil had more favorable sensory peroperties than the sample containing this oil in free form. However, the free form showed higher antibacterial activity but in the regard to the protection of bioactivity of oils from the undesirable condition, controlled release and marketability of product, loading the oils in alginate bead is a suitable way for application of black seed and linseed oil in food products.

Today, with the rise of awareness and changing attitudes, the demand for healthy foods has increased. The effective compounds of black seed with its antioxidant and antimicrobial properties are of interest to many researchers. The aim of this study was to benefit from the functional properties of black seed oil in a food model. In the first phase, the peroxide index, color and antimicrobial properties of free and alginate encapsulated black seed oil were investigated. In the second phase, microbial and sensory properties of 4 chocolate ganache samples (preservative free (G or Control), preservative free + inoculated microorganisms (GM), containing 3% free black seed oil + inoculated microorganisms (GOM) And containing 3% of encapsulated black seed oil + inoculated microorganisms (GOM)) were compared. The results of the first phase showed that MIC and MBC of free black seed oil on Salmonella typhimurium, Escherichia coli, Staphylococcus aureus, Aspergillus niger and Candida albicans were higher than the encapsulated black seed oil. Also, the highest and lowest antimicrobial effects of black seed oil were observed on Candida albicans and Escherichia coli, respectively. The peroxide index and color of free black seed oil was more than encapsulated black seed oil . Also, the results of the second phase showed that the presence of black seed oil in the chocolate ganache formulation reduced the microbial load of the produced samples. However, the antimicrobial action of free black seed oil on the microbial load of the food model was more than encapsulated black seed oil. Finally, the results of sensory evaluation showed that chocolate ganache containing encapsulated black seed oil had better sensory properties compared to the sample containing free black seed oil.

In recent decades, the demand for the use of functional compounds in food and pharmaceutical products has increased. The aim of this study was to compare the phytochemical compounds and antioxidant, anti-Alzheimer's and antimicrobial properties of Padina distromatic and Sargassum angustifulium algae extracted by ultrasound. The results showed that butanoic acid was the most important substance in Padina and Sargasum extracts. Total content of polyphenols, flavonoids and IC50 index of Padina extract and sargasum 43.45 and 46.63 mg GAE/g, flavonoid content 24.59 and 55.40 mg QE/g and IC50 index 10.38 and 30.77 g/ml respectively. The inhibitory activity of acetylcholinesterase (AChE) of Padina and Sargassum algae extracts was 42.5% and 25.16%, respectively. Nitric oxide (NO) inhibitory results showed that nitric oxide inhibitory activity increased with increasing the concentration of both Padina and Sargassum algae extracts (from 50 to 400 mg / ml). However, Padina algae extract had the strongest NO inhibitory activity at all concentrations. Padina extract had more antimicrobial properties compared to the algae extract of sargassum. The minimum inhibitory concentration (MIC) of Padina extract at 12.5 mg/ml was obtained on Staphylococcus aureus. Padina extract at 50 mg/ml had a bactericidal effect on Staphylococcus aureus. Finally, Sargassum extract should be used as natural antioxidant and Padina extract as natural anti-Alzheimer's and antimicrobial components.

Promising secondary metabolites of brown algae have been given particular importance, due to their various biological activities. In the present study, two extraction methods including maceration and ultrasound were performed to prepare extracts from brown algae including Padina distromatic, then, aimed to evaluate antioxidant, antimicrobial, and anti-Alzheimer’s activities of extracts. Padina algae extract prepared by ultrasound method had higher total phenol (43.25 mg GAE/g) and flavonoid content (24.59 mg QE/gDA). However, the maximum antioxidant activity was observed in algae extract prepared by maceration method. Padina algae extract prepared by ultrasound method had the highest inhibitory activity of acetylcholinesterase and the strongest inhibition of nitric oxide production was observed in this extract. Escherichia coli and Listeria innocua bacteria were more resistant to Padina algae extract than other microorganisms and the results of microbial test showed that Padina algae extract prepared by ultrasound method was more antimicrobial than maceration method. Collectively, the obtained results provide valuable evidence for antioxidant, anti-Alzheimer, and antimicrobial activity driven by Padina distromatic extracts which can highlight their possible approach in the therapeutic utilization.