solin ghaderi

Type 1 diabetes mellitus (T1DM) occurs due to the decrease in insulin secretion following the destruction of pancreatic beta cells. This disease is increasing worldwide, especially among children under the age of 5 years, which is usually associated with irreversible complications such as hepatopathy and nephropathy. The present study aimed to investigate the antidiabetic effect of the heat-killed Actinomycetales species, including Gordonia bronchialis (Gb), and Tsukamurella inchonensis (Ti) in streptozotocin-diabetic rats by oral administration. This experiment was performed in six groups, including healthy control, diabetic control, low-dose Gb (G1), high-dose Gb (G2), low-dose-Ti (T1), and high-dose Ti (T2). Subsequently; the levels of ALT, AST, total protein, albumin, BUN, creatinine, CRP, IL-1β, and IL-2 were measured in the serum samples in the 14th and 21st days. Besides, histopathological lesions were studied in the liver and kidney. Our findings showed that Gb and Ti could alter the examined serum parameters, particularly in the T2 groups. Also, histological examination revealed a remarkable attenuation in the pathological lesions such as focal necrosis, vascular congestion, and hemorrhage in the liver and kidney of the treated rats by Gb and Ti. Here, it is concluded that oral administration of the heat-killed Actinomycetales species, particularly with a high dose of Ti, could beneficially improve the progression of T1DM and its various complications, which can be used to treat T1DM in the future.

Type 1 diabetes mellitus (T1DM) has dramatically increased in recent years, especially in young people, and limits the life quality of the patients involved. Thus, many researchers are performing extensive studies to find alternative treatments for DM.

Here, we evaluated the improvement effects of the heat-killed Actinomycetales species, including Gordonia bronchialis, and Tsukamurella inchonensis in streptozotocin-diabetic rats by biochemical, immunological, and histopathological examinations.

The present findings exhibited a dramatic and progressive alteration in the serum levels of IL-6, IL-10 and TNF-α in the diabetic group, which were related to the blood glucose and insulin levels, oxidative stress defense (evaluated by TAC and MDA activities), and the pancreas biochemical indicators (such as amylase and lipase). More importantly, the present results were consistent with the histopathological findings, which included cellular degeneration, vascular congestion, hemorrhage, focal necrosis associated with mononuclear cell infiltration. Interestingly, all of the diabetic changes in the blood serum and tissues improved remarkably in the treated groups by Actinomycetales species.

Surprisingly, most of the current diabetic complications effectively attenuated after oral administration of both Actinomycetales species, particularly with a high dose of T. inchonensis. Thus, it is concluded that the heat-killed Actinomycetales species can prevent and improve the progression of T1DM and its various complications profoundly.

This study evaluated the possible protective effects of Gordonia bronchialis (Gb) on oxidative stress and some subsequent alterations on testis from rats undergoing an experimentally induced type 1 diabetes.

A total of 40 male rats were randomly divided into four groups of ten. Diabetes was induced by injection of 55 mg/kg streptozotocin in 30 rats. Oral administration of Gb at dose of 105 (low dose) and 107 (high dose) CFU/rat was performed in two groups continuously for 14 days. The third and fourth groups received normal saline as the diabetic and healthy control groups, respectively. The blood and testicular tissue samples were taken on the 14th and 21st days post treatment for biochemical and histopathological evaluations.

Significant differences were found in blood glucose level, insulin, IL-6 and TNF-α values together with catalase and superoxide dismutase activities and malondialdehyde level in the diabetic group in comparison with healthy and Gb recipient groups. Moreover, the histopathological lesions observed in the diabetic rats mainly included basement membrane thickening, decreased number of Sertoli cells, and severe reduction of spermatogenesis markedly attenuated in Gb-treated rats.

Taken together, it seems that oral administration of Gb could ameliorate testicular damage associated with some related parameters in the diabetic animal model.