somayeh aminizadeh
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پیاز یکی از سبزی های اصلی و پرمصرف در جهان است که هر ساله ژنوتیپ های زیادی اعم از بومی و وارداتی در کشور کشت می شوند. استان های جنوبی کشور حدود 50 درصد از سطح زیر کشت را به کشت پیاز اختصاص داده اند. بنابراین، گزینش ژنوتیپ های برتر سازگار با مناطق جنوبی برای حصول حداکثر عملکرد امری ضروری است. در این مطالعه، با هدف بررسی سازگاری ارقام پیاز خارجی (چهار رقم پیاز سفید و چهار رقم پیاز زرد) در شرایط آب و هوایی جنوب استان کرمان، آزمایشی در قالب طرح مربع لاتین با چهار تکرار در ایستگاه تحقیقاتی واقع در جنوب استان کرمان انجام شد. صفات فنوتیپی شامل: تعداد روز تا سوخ دهی، تعداد روز تا رسیدن، قطر غده، طول غده، ارتفاع بوته، تعداد لایه های خوراکی، وزن تر بوته، وزن خشک بوته، طول برگ، درصد آلودگی به فوزاریوم و عملکرد غده مورد ارزیابی قرار گرفتند. نتایج تجزیه واریانس تفاوت معنی داری در سطح یک درصد بین ارقام پیاز سفید و زرد از لحاظ صفات مورفولوژیک نشان داد. براساس نتایج به دست آمده، ارقام مینروا (پیاز سفید) و ریوبراوو(پیاز زرد) به عنوان ارقام زودرس و با درصد آلودگی کمتر به فوزاریوم معرفی شدند و ارقام سوبرانا (پیاز زرد) و سایروس (پیاز سفید) به عنوان ارقام با عملکرد غده بالابرای کاشت در مناطق جنوبی استان کرمان و همچنین برای استفاده در برنامه های به نژادی پیاز معرفی گردیدند. در هر دو نوع پیاز قطر غده، طول غده، وزن تر بوته و وزن خشک بوته همبستگی بالا، مثبت و معنی داری با عملکرد غده نشان دادند، در نتیجه برای افزایش عملکرد و اهداف به نژادی می توان به گزینش پیازهایی با قطر و وزن خشک بوته بیشتر اقدام نمود.
کلید واژگان: تنوع ژنتیکی, زودرسی, عملکرد, وزن غدهIntroductionOnion stands as one of the primary and extensively consumed vegetables at global scale, with numerous genotypes, both native and imported, being cultivated in the country annually. The southern provinces of the country have allocated about 50 percent of the cultivated area to onion cultivation. Therefore, selecting superior genotypes compatible with southern regions is essential to achieve maximum yield. Considering the long history of onion cultivation as one of the native plants of our country, there is a lot of genetic diversity in this plant. However, to date, inadequate breeding programs have been conducted to develop new, high-yield varieties in our country, leading most farmers to rely on local cultivars for cultivation. Therefore, it is necessary to design targeted breeding programs to produce new varieties superior in yield potential, flavor, aroma, resistance to pests and diseases, and other characteristics. In this regard, collecting diverse onion germplasm and evaluating genetic diversity in it is one of the basic steps for designing targeted breeding. The main purpose of this project is to evaluate genetic diversity in yellow and white onion cultivars in terms of morphological traits and selecting superior genotypes. The results of this project are a necessary prerequisite for long-term plans for the production of new varieties of onions.
Materials and MethodsThe research was carried out in the Faryab region, situated in the southern part of Kerman province. We assessed four commercial yellow onion hybrids- Rio Bravo, Soberana, Eiden, and Ascro Goldeneye -and four commercial white onion hybrids-Minerva, Cirrus, Macran, and 4043-using a 4x4 Latin square design during the 2022 growing season. Various observable traits, such as days to bulbing, days to maturity, onion bulb diameter, plant height and leaf length, fresh and dry plant weight, number of edible layers, fusarium infection percentage, and average tuber yield were recorded. To analyze the data, we used descriptive statistics to show the maximum, minimum, and average values of the traits, along with variance analysis, correlation analysis, and mean comparisons.
Results and DiscussionThe analysis of variance revealed significant variations across the measured traits. Within the yellow onion genotypes, Rio Bravo displayed the shortest maturity cycle, averaging 113 days to maturity, while Aiden exhibited the longest maturity duration with an average of 141 days. The Sobrana cultivar consistently outperformed other varieties across all investigated traits and was introduced as the best yellow onion cultivar. Bulb yield displayed strong and positive correlations with bulb diameter, bulb length, plant height, leaf length, number of edible layers, and both fresh and dry plant weight. However, the correlation between days to maturity and bulb yield was low, suggesting that the duration from planting to maturity did not affect the enhancement of bulb yield in yellow onions. The findings indicated that in contrast to yellow onion cultivars, white onion cultivars exhibited a longer average duration to bulb formation and maturity, alongside a lower bulb yield. Among the white onion genotypes, Minerva displayed the earliest maturity, while Cirrus was observed as the longest maturing genotype. Cirrus cultivar exhibited the highest average bulb diameter, bulb length, plant fresh weight, plant dry weight, leaf length, and bulb yield. Conversely, the Macran genotype displayed the lowest mean values for bulb diameter, bulb length, plant fresh weight, plant dry weight, and bulb yield. Additionally, a significant and strong positive relationship was observed among bulb diameter, bulb length, plant fresh weight, and plant dry weight. Typically, onion quality is often assessed based on bulb diameter, where larger bulb diameters tend to correspond with higher bulb yields. Evidently, both in yellow and white onion cultivars, those with higher yields also demonstrated larger bulb diameters.
ConclusionIn the southern regions of Kerman province, the early maturity of onions is very important. Among the yellow onion genotypes, Rio Bravo was the earliest variety. The Soberana cultivar had a higher bulb yield compared to Rio Bravo, but it matured approximately twenty days later than Rio Bravo. Among the white varieties, Minerva was also the earliest maturing variety. However, the Cirrus cultivar had a higher bulb yield compared to Minerva. Yet, in comparison to Minerva, it was both later to mature and more susceptible to fusarium. The selection of onion varieties can significantly depend on the specific conditions of the region and the needs of the farmers. If timing and fusarium sensitivity are crucial factors for farmers, Minerva and Rio Bravo, with lower susceptibility to fusarium, might be suitable options. However, if bulb yield and product quality hold greater importance for farmers, Soberana among yellow onions and Cirrus among white onions are recommended. Additionally, considering breeding goals, these varieties can be utilized in onion breeding programs according to breeding objectives.
Keywords: Bulb Weight, Earliness, Genetic Diversity, Yield -
هدف
زودرسی به عنوان مکانیزم سازگاری در محیط هایی با گرما و خشکی آخر فصل و یک صفت مهم در برنامه های به نژادی گندم است. Ppd و Vrn مهم ترین ژن های کنترل کننده زودرسی هستند. هدف از این تحقیق بررسی تاثیر ژن های Ppd و Vrn بر زودرسی، عملکرد و صفات مهم زراعی در نسل BC4F2 حاصل از تلاقی برگشتی روشن (والد تکراری) و اکسکلیبر بود.
مواد و روش هاجمعیت BC4F2 حاصل از تلاقی روشن و اکسکلیبر در دانشگاه شهید باهنر کرمان ایجاد شد. در این برنامه ی به نژادی، صفت زود گلدهی از رقم اکسکلیبر به رقم روشن انتقال داده شد (روشن به عنوان والد تکراری بود). این جمعیت ، شامل 175 ژنوتیپ نسل BC4F2، و والدین آنها در سال زراعی 1399-1398 در مزرعه تحقیقاتی دانشگاه شهید باهنر کرمان کشت شدند. والدین و نتاج با استفاده از نشانگرهای اختصاصی Vrn و Ppd ژنوتیپ یابی شدند. در این جمعیت زودرسی، عملکرد و صفات مهم زراعی بررسی شد.
نتایجدر جایگاه ژنی Ppd-1، رقم اکسکلیبر دارای آلل Ppd-D1a و رقم روشن دارای آلل Ppd-D1b بود. فراوانی آلل Ppd-D1a در جمعیت مورد بررسی 33/27 درصد، و بیش ترین فراوانی آللی مربوط به آلل های هتروزیگوت (45 درصد) بود. در جمعیت مورد بررسی ژنوتیپ Ppd-D1a/ Ppd-D1a نسبت به /Ppd-D1b Ppd-D1b به طور متوسط 6 روز و نسبت به ژنوتیپ هتروزیگوت به طور متوسط 4 روز زودتر وارد فاز زایشی شدند. تغییرات آللی در مکان ژنی Vrn -1 در جمعیت مورد مطالعه نشان می دهد که بین نتاج در این تلاقی تنوع آللی وجود ندارد. زود سنبله دهی با عملکرد دانه، وزن 1000 دانه، تعداد سنبله بارور و تعداد دانه در بوته همبستگی منفی و معنی داری داشت.
نتیجه گیریاین نتایج اهمیت ژن Ppd-D1a در زودرسی و بهبود عملکرد و اجزای عملکرد را تایید می کند. گزینش برای زودسنبله دهی باعث افزایش عملکرد دانه، وزن 1000 دانه، تعداد سنبله بارور، عملکرد بیولوژیک و وزن خشک سنبله شد. با توجه به تاثیر بزرگ و معنی دار ژن Ppd-D1a بر عملکرد و اجزای عملکرد، توصیه می گردد در برنامه های به نژادی گندم، گزینش به کمک نشانگر این ژن مورد توجه قرار گیرد. تا قبل از نسل BC4F2 گزینش فنوتیپی انجام شد و تنوع ژن Vrn در جمعیت تثبیت شده بود.
کلید واژگان: تلاقی برگشتی به کمک نشانگر, زود گلدهی, ژن های فتوپریود (Ppd), ژن های ورنالیزاسیون (Vrn), گندم نانObjectiveEarly heading as an adaptation mechanism for end season heat and drought stress is an important goal in wheat breeding programs. Photoperiod (Ppd) and vernalization (Vrn) are the most important genes controlling early heading in bread wheat. The aim of the present study was to investigate the effect of Ppd and Vrn genes on early heading, grain yield and important agronomic traits in BC4F2 generation of Roshan (recurrent parent) and Excalibur backcross.
Materials and methodsA BC4F2 population resulted from backcross of Roshan and Excalibur was generated in Shahid Bahonar University of Kerman. In this breeding program, early heading was transferred from Excalibur cultivar to Roshan (recurrent parent). This population, including 175 BC4F2 progenies, and their parents were cultivated in the research field of Shahid Bahonar University of Kerman in the 2019-2020 growing season. The progenies were genotyping using specific markers Vrn-B1, Vrn-D1 and Ppd-D1. In this population, early heading, grain yield and important agronomic traits were evaluated.
ResultsExcalibur and Roshan had Ppd-D1a, and Ppd-D1b alleles, respectively. The frequency of Ppd-D1a/Ppd-D1a and heterozygous genotypes in the evaluated population were 27.33% and 415%, respectively. In this population, Ppd-D1a/Ppd-D1a, which is photoperiod insensitive genotype, was 6 and 4 days earlier heading than Ppd-D1b/Ppd-D1b and Ppd-D1a/Ppd-D1b genotypes, respectively. There was no allelic diversity between progeny for Vrn-1 locus. Early heading had a significant negative correlation with grain yield, 1000-grain weight, spikes number and grains number per spike.
ConclusionsThe results confirm the importance of Ppd-D1a gene in the early heading, grain yield and yield components. Selection for early heading increased grain yield, 1000-grain weight, spikes number, biological yield, stover biomass at harvest and spikes dry weight. Due to the large and significant effect of Ppd-D1a gene on grain yield and yield components, we recommend marker assisted selection of this gene in wheat breeding programs. Phenotypic selection was performed before BC4F2 generation and Vrn gene diversity was established in the population.
Keywords: Bread wheat, Early heading, Marker assisted backcrossing (MAB), photoperiod genes (Ppd), Vernalization genes (Vrn) -
BackgroundAlthough natural disasters leave thousands of deaths and wounded, one of their most significant outcomes is empowering women as well as taking measures with special attention to women. This study aimed to analyze womens cooperation and its affective factors in natural disasters and accidents.Materials And MethodsThe study design is cross-sectional and descriptive. The study population comprised the women living in Bam, Iran and the sample size was calculated as 385. The study instrument is a researcher-made questionnaire. To analyze the data, the descriptive statistical methods, the statistical correlation test, and 1-way analysis of variance were performed by using SPSS 16.ResultsThe study result about the effects of the women demographic characteristics on their cooperation showed that there were no relationships between womens cooperation and their marital status or degree of education. However, there were significant and positive relationships between their cooperation and variables of age, being indigenous, and occupational status.
The results have also indicated that there were significant relationships between womens cooperation and variables of knowledge and attitude.ConclusionWomen, despite their vulnerability in natural accidents, have the capability in performing different tasks such as taking care and accommodating the injured family members, and making peace and relief in difficult and undesirable situations after natural disasters such as earthquake. However, the results of this study revealed that womens cooperation in different parts of crisis management was very limited. This matter demands more attention of the responsible authorities.Keywords: Natural disasters, accidents, Women, Cooperation
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