فهرست مطالب teguh wahju sardjono

The purpose of this study is to prove the efficacy of Curcuma longa (turmeric) extracts against soot (carbon black)-exposed rats with Ox-LDL and eNOS levels. A total of 30 rats were divided into 5 groups with 6 rats in each group. Negative control group (C-) received no treatment. The positive control group (C+) exposed to soot at a concentration of 1064 mg/m3 for 8 hours. Treatment group T1 was exposed to soot at 1064 mg/m3 for 8 hours + Curcuma longa at 1 mg/kg body weight. T2 group was exposed to soot at 1064 mg/m3 for 8 hours + 2 mg curcuma longa mg/kg body weight, and T3 group were exposed to soot at of 1064 mg/m3 for 8 hours + curcuma longa 3 mg/kg body weight. The result of T3 group had lower Ox-LDL levels and higher eNOS levels, and also the difference was significant (p>0.05) than the C+ group. We conclude that the treatment of rats exposed to 1064 mg/m3 soot particles for 8 hours with Curcuma longa extract at a dose of 3 mg/kg body weight reduced Ox-LDL levels and increased eNOS levels because curcumin from Curcuma longa extract effective to break the chain reaction from lipid peroxidation, inhibit LOX-1 expression, prevent LDL modification into ox-LDL, and decrease coupled eNOS levels that prevent NO and GSH degradation.

Placental malaria involves the sequestration of infected erythrocytes and infiltration of monocytes, helper T cells (CD4), cytotoxic T cells (CD8) as well as T-cell intracellular antigen-1 (TIA-1) in placental intervillous space. These may interferes the nutrient and oxygen transport, causing placental hypoxia and insufficiency that may affect the fetal growth. This study aimed to prove whether the infiltration of lymphocytes in placental malaria mice increases the expression of HIF-1α thus causes fetal Low Birth Weight (LBW).

Nine pregnant BALB/c mice that infected with Plasmodium berghei ANKA strain on day 9 post mating were used as treatment group and 8 non infected pregnant mice were used as control group. The mice were sacrificed on day 18 post mating; then the fetus was weighed individually and the placentas were isolated separately. Expression of CD4, CD8 and HIF-1α were counted by immunohistochemistry using CD4 monoclonal Ab (Santa cruz, sc-59031 CD4) and CD 8 monoclonal Ab (NeoMarker RM-9116-S0) as well as anti-HIF-1α antibody (H1α67) ChIP Grade from Abcam.

There was a higher expression of CD8, CD4 and HIF-1α in infected placenta compare to normal placenta. Analysis using Structural Equation Modeling (SEM) showed expression CD8 and CD4 caused an increase expression of HIF-1α in placenta (t ≥1.96). Expression of HIF-1α caused low fetal weight (t ≥1.96).

In placental malaria, the expression of CD4 and CD8 induce placental hypoxia characterized by increased expression of HIF-1α that causes LBW.