فهرست مطالب yudha endra pratama

Dadiah is one of traditional food in West Sumatera. It is made from naturally fermented buffalo milk in bamboo tubes by lactic acid bacteria found naturally in the buffalo milk and bamboo tubes themselves. Lactic  acid bacteria strains are the main probiotics in the food and pharmaceutical markets. Probiotics are live microorganisms or components of a microbial cell that give good effect to other organisms. Probiotics have an important role in the health of the gastrointestinal tract in general. Pediococcus acidilactici is one of the lactic acid bacteria that acts as a probiotic isolated from Dadiah. Insulin is a hormone produced by pancreatic beta cells, which plays an important role in regulating blood glucose levels, so that they remain in a normal state, and its promoter regulates the rate of transcription in response to physiological regulators. In human pancreatic cells, the insulin promoter interacts with loci associated with diabetes risk and controls genes involved in insulin metabolism. Insulin promoters are tissue-specific and typically only activate in islet beta cells in adult tissues to stimulate insulin production. This study aims to determine the secondary metabolites of P. acidilactici to insulin promotor expression by looking at the canonical and isomeric SMILE (simplified molecular-input line-entry system) structures in the PubChem database. After conducting bioinformatics analysis to determine the secondary metabolites of P. acidilactici, its compound has potential as a diabetes treatment, because the QSAR results show potential as an insulin promoter (0.75).

Dadiah is a traditional food for the people of West Sumatra made from fermented buffalo milk in a bamboo container. As a fermented product, dadiah contains many lactic acid bacteria that have the potential as probiotics, including Lactobacillus plantarum isolated from dadiah payakumbuh which has the potential as a probiotic. As a probiotic, L. plantarum exhibits a number of beneficial health effects in humans and animals. It is capable of enhancing the body's immune system or as an immune-modulator. Probiotics help regulate the intestinal microflora and have immunomodulatory properties. This study aims to determine the effectiveness of L. plantarum from dadiah payakumbuh as an immune-modulator, both bio-informatically and in vitro. This study looked at the activity of L. plantarum as an immune-modulator in bioinformatics by searching for secondary metabolites by looking at the structure of the canonical and isomeric SMILE (simplified molecular-input line-entry system) in the PubChem database, as well as analyzing IgA in jejunum tissue of Norway Rat White (Rattus novernicus) given hypertension treatment using KIT ELISA. It was found that there was a predictive result of SAR Way2 Drug pass server from L. plantarum as an immune-modulator with a value of 0.47 and the IgA value in the jejunum tissue of R. novernicus. Hypertension in the rats increased compared to those who were not treated. L. plantarum from dadiah payakumbuh is able to increase the body immunity, since it produces immunomodulatory secondary metabolites.