z. davood abadi farahani

The aim of this study was to investigate the effect of aerobic exercise on HSPA2 expression in testes and sperm parameters in diabetic rats.

Two-month-old male wistar rats were randomly divided into three groups (n=10 in each group): control (C), diabetic (D) and diabetic training (DT). Diabetes was induced with a single intraperitoneal injection of streptozotocin. The DTrats were conditioned to run on a treadmill for the 8-week period. 48 hours after the last session, the testes were removed, and subjected to histological examination, semen analysis and total RNA was isolated from testes to measure by RT-qPCRHSPA2 mRNA expression level. The variance analysis test were applied to analyze the data (P<0.05).

Findings show that the D group had a decrease in sperm count (P=0.01), motility (P=0.001), as well as increase in abnormal sperm morphology (P=0.001). Furthermore, in the D group, HSPA2 expressionin testicular tissue was significantly reduced (P=0.01). Unlike the D group, in the DT group sperm count (P=0.04) and viability (P=0.02), and HSPA2 expression (P=0.03) were elevated.

In rat testes, diabetes down regulates HSPA2 expression, which is collectively detrimental to semen parameters. Exercise protected effectively against this detrimental effect.