zahra razzaghi

Evaluating pseudomotor performance can be a valuable tool for investigating the peripheral autonomic nervous system in diabetic patients. Sudoscan, a simple and non-invasive method for assessing pseudomotor performance, has been developed in recent years. This study aimed to investigate autonomic neuropathy using Sudoscan in diabetic patients with lower urinary tract symptoms (LUTS) of unknown cause.

In this cross-sectional study conducted from April 2022 to April 2023, we included 195 patients with type 2 diabetes who were referred to the urology clinic. We extracted demographic, clinical, and laboratory data from the patient files and evaluated urinary symptoms using the International Prostate Symptom Score (IPSS) questionnaire. Patients underwent Sudoscan testing to evaluate autonomic neuropathy in the physical medicine and rehabilitation clinic. To further assess urinary irritative symptoms, patients underwent urodynamic studies (UDS) and ultrasonography.

The Sudoscan test results showed that autonomic neuropathy was present in 77 patients (40%), with 43 (22.1%) having moderate and 44 (22.6%) having severe neuropathy. Patients with autonomic neuropathy were found to be older, had longer diabetes durations, higher average blood glucose levels, and higher creatinine levels. Additionally, we found a significant correlation between autonomic neuropathy and signs of high post-void residue on ultrasound and detrusor contraction disorders on UDS (p-value < 0.05).

Our study found a higher prevalence of autonomic neuropathy in diabetic patients with LUTS using Sudoscan (40%). Longer diabetes duration and poor glycemic control were associated with an increased risk of autonomic neuropathy linked with LUTS, such as urge incontinence.

Alcohol is a risk factor for liver diseases. There is a correlation between alcohol consumption and fatty liver disease. Experiences have shown gene expression alteration in the liver following alcohol consumption. Since the molecular mechanism of connection between alcohol consumption and fatty liver disease needs a clear perspective, this study aims to explore the significant genes that are targeted by alcohol in the liver of mice.

Gene expression profiles of mice livers fed with alcohol were retrieved from the Gene Expression Omnibus (GEO) database and compared with the control samples. Data are pre-evaluated with the GEO2R program, and the significant differentially expressed genes (DEGs) are analyzed via gene expression evaluations and regulatory network assessment.

Among the 25619 dysregulated genes, 78 significant DEGs were pointed out. Gene expression analysis showed that most extremely dysregulated genes are up-regulated and belong to the cytochrome P450 genes family. Finally, cytochrome P450 and glutathione S-transferase genes family, as well as hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 4, were introduced as the critical targeted genes.

In conclusion, detoxification of xenobiotics, cellular metabolism and homeostasis, the pathogenesis of some liver diseases, synthesis of several prostaglandins and steroid hormones, and inflammation fibrosis in the liver are possibly associated with alcohol consumption.

 Intensity is one of the important parameters of laser radiation in photodynamic therapy. Effective treatment requires the selection of a suitable power of laser. This study aimed to evaluate laser effectiveness in photodynamic therapy via high and low intensity by the analysis of the gene expression profiles of the treated cells.

 The gene expression profiles of human SK-ChA-1 cells which are treated by 500mW and 50mW laser radiation were retrieved from the Gene Expression Omnibus (GEO) database. Data were assessed by the GEO2R program, and the significant differentially expressed genes (DEGs) were investigated via expression examination and protein-protein interaction (PPI) network analysis.

 Analyses revealed that the higher intensity of radiation is associated with wide gene expression changes relative to the lower mode. 196 significant DEGs were identified and assessed. The extremely dysregulated DEGs except MMP1 were down-regulated. STAT1, IRF7, IL1B, DDX58, ISG15, RSAD2, DHX58, OASL, OAS1, STAT2, DDX60, OAS2, USP18, and IFI44L were introduced as hubs of the main component of the PPI network. Final analysis showed that STAT1, IRF7, IL1B, DDX58, and STAT2 are the critical DEGs.

 Compared to the 50 mW mode of radiation, 500 mW laser intensity effectively changed apoptosis, differentiation, cell proliferation and angiogenesis, regulation of other inflammation-related molecules, innate immunity, and maintaining immune homeostasis.

 Psoriasis is a common autoimmune skin disease associated with genetically influenced chronic inflammation accompanied by remitting and deteriorating scaly skin. T-cell targeted biologics, IL-17 inhibitors, IL 12/IL-23 inhibitors, TNF-α inhibitors, PDE4 inhibitors, and ultraviolet (UV) radiation are applied to treat psoriasis. Efficacy evaluation of narrow-band UVB (NB-UVB) radiation was the aim of this study.

 Data were extracted from Gene Expression Omnibus (GEO) and were pre-evaluated via the GEO2R program. The significant differentially expressed genes (DEGs) were included in the protein-protein interaction (PPI) network analysis. The hubs, bottlenecks, and hub-bottleneck DEGs were introduced as central genes. Activation, inhibition, and expression relationship between central genes were assessed to explore the critical individuals.

 Among 513 analyzed significant DEGs, 22 hub-bottleneck genes were identified. Further analysis revealed that FN1, STAT3, HIF1A, IL1B, P4HB, SOD2, MMP2, and STAT1 were the crucial genes in psoriasis samples targeted by NB-UVB radiation.

 In conclusion, NB-UVB radiation as treatment targets critical genes in peri-lesion skin tissue biopsy of psoriasis patients via a complicated mechanism. This therapeutic method downregulates STAT3, HIF1A, IL1B, and P4HB to treat psoriasis but downregulates STAT1 and SOD2 and upregulates MMP2 and FN1 to develop disease.

 Atopic dermatitis is a common inflammatory skin disease which is treated with narrowband ultraviolet B (NB-UVB). Exploring the critical targeted genes in patients by UV radiation is the main aim of this study.

 Gene expression profiles of lesional and non-lesional skin samples of atopic dermatitis patients after treatment with NB-UVB and the non-irradiated samples were extracted from the Gene Expression Omnibus (GEO) database and analyzed via protein-protein interaction (PPI) network analysis to find the critical targeted genes.

 A total of 357 significant differentially expressed genes (DEGs) were included in the PPI network. CTNNB1, SRSF1, YWHAB, SMC3, GNB2, ARF3, UBL7, RAB2A, YWHAE, EIF5B, SNRPE, PPIG, RC3H2, CFL1, SMARCB1. LAPTM5, PRPF40A, and RBBP4 were introduced as hub bottlenecks.

 In conclusion, five central genes including SMC3, ARF3, EIF5B, SMARCB1, and LAPTM5 were highlighted as critical genes in response to NB-UVB radiation in the skin of the treated atopic dermatitis patients. The introduced crucial genes are involved in essential cellular functions such as apoptosis, cell cycle, cell proliferation, and inflammation. It seems that applied NB-UVB radiation is a suitable therapeutic method for atopic dermatitis disease.

 Photothermal therapy (PTT) by using a near-infrared (NIR) laser, as a successful treatment of cancer, has attracted extensive attention of researchers. Its advantages as a noninvasive and suitable method have been confirmed. Discovery of the NIR laser molecular mechanism at the cellular level via system biology assessment to identify the crucial targeted genes is the aim of this study.

 RNA-seq series of six samples were retrieved from Gene Expression Omnibus (GEO) and pre-evaluated by the GEO2R program for more analysis. The significant differentially expressed genes (DEGs) were determined and studied via gene expression analysis, proteinprotein interaction (PPI) network assessment, action map evaluation, and gene ontology enrichment.

 HSPA5, DDIT3, TRIB3, PTGS2, HMOX1, ASNS, GDF15, SLC7A11, and SQSTM1 were identified as central genes. Comparing the central genes and the determined crucial genes via gene expression analysis, actin map results, and gene ontology enrichment led to the introduction of HSPA5, DDIT3, PTGS2, HMOX1, and GDF15 as critical genes in response to the NIR laser.

 The results indicated that the principle biological process “Endoplasmic reticulum unfolded protein response” and HSPA5, DDIT3, PTGS2, HMOX1, and GDF15 are the crucial
targets of the NIR laser. The results also showed that the NIR laser induces stress conditions in the irradiated cells.

 The retina is a light-sensitive tissue, and intensive light exposure leads to lightinduced retinal damage. It is pointed out that photoreceptor damage is responsible for the decrease in retina function. The aim of this study was to detect the main genes and biological terms which are involved in retinal response to intensive light exposure.

 The effect of intensive light on the mouse retina function was searched in the Gene Expression Omnibus (GEO) database. The data of GSE22818 were assessed by the GEO2R program. The significant differentially expressed genes (DEGs) were determined and evaluated via directed protein-protein interaction (PPI) network analysis. The critical significant DEGs were enriched via gene ontology analysis to find the related biological processes, molecular function, and biochemical pathways.

 Data analysis indicates that the high intensity of light induces gene expression alteration in the retina. 105 significant DEGs were identified as the main responsive genes to light damage in the retina. STAT3, JUN, IL6ST, SOCS3, ATF3, JUNB, FOSL1, CCL2, ICAM1, FGF2, AGT, MYC, LIF, CISH, and EGR1 were introduced as the critical affected genes. STAT3, JUN, IL6ST, SOCS3, and ATF3 and “Positive regulation of the receptor signaling pathway via JAK-STAT” were highlighted as the key elements of molecular events.

 It can be concluded that regulation of the key DEGs and the dependent biological terms can effectively provide tools to prevent the development of light-induced retinal damage.

Erectile dysfunction (ED) in men is a significant issue that can profoundly impact personal relationships, mood, and overall quality of life. The nocturnal penile tumescence (NPT) test is a valuable tool for distinguishing between psychological and physiological causes of ED. The normal values of the NPT test are a subject of debate across various racial groups. Therefore, there is a need to conduct a study in the Middle East region to establish standard norms for NPT.

The aim of this study is to investigate the results of the NPT test in sexually healthy Iranian men.

This descriptive study involved the examination of 30 sexually healthy Iranian volunteers using the iranian erection analyzer over a period of two nights. In this study, a NPT episode was defined as radial rigidity exceeding 70%. The frequency and duration of these episodes were documented and subjected to analysis.

The average number of tumescence episodes per participant on the first and second nights was observed to be 1.73 ± 0.82 and 1.9 ± 0.66 episodes, respectively. The average duration of each tumescence episode on the first and second nights was found to be 16.04 ± 7.7 and 22.08 ± 6.85 minutes, respectively. A statistically significant difference in tumescence duration was noted between the two nights (P < 0.001), with the second night showing higher values. Furthermore, it was determined that 83.4% of sexually healthy men experienced 1 to 2 episodes of tumescence during the night.

The findings of this study indicate that the majority of sexually healthy Iranian men experience 1 to 2 episodes of NPT with rigidity surpassing 70% overnight, with an average duration of 16 to 22 minutes per episode. Additionally, the study suggests that relying on a single-night NPT test may be inadequate for an accurate assessment.

Rutin is a lipophilic natural flavonoid. It is found in vegetables, citrus fruits, and beverages. This study aims to evaluate rutin metabolic pathways in human senescent stromal cells. Data are extracted from the Gene Expression Omnibus (GEO) database and pre-evaluated via GEO2R to find the significant differentially expressed genes (DEGs). The significant DEGs were assessed via protein-protein interaction PPI network analysis to explore the hub genes. The hubs were screened via directed PPI to find the critical DEGs. Data were assessed via volcano plot, Uniform Manifold Approximation and Projection (UMAP) plot, and Venn diagram. A total number of 9124 significant DEGs were analyzed to determine 33 upregulated and 61 downregulated hubs. The identified hubs were investigated via directed PPI and Il1B. ICAM1, CCL2, EGF, CXCL8, PTGS2, CAMK2B, CCN2, VCAM1, ELN, CXCL12, BGN, and TLR4 were pointed out as critical hub genes. Il1B, CCL2, GNAO1, ICAM1, EGF, and CXCL8 appeared as controller genes affected by rutin while PTGS2 and CAMK2B were the most controlled individuals. The finding refers to the significant advantages of the rutin effect on the function of treated cells. These advantages are corresponded to the usefulness of rutin as an herbal drug candidate. However, more investigations are required to decrease its side effects.

This study aimed to introduce a biomarker panel to detect pancreatic ductal adenocarcinoma (PDAC) in the early stage, and also differentiate of stages from each other.

PDAC is a lethal cancer with poor prognosis and overall survival.

Gene expression profiles of PDAC patients were extracted from the Gene Expression Omnibus (GEO) database. The genes that were significantly differentially expressed (DEGs) for Stages I, II, and III in comparison to the healthy controls were identified. The determined DEGs were assessed via protein–protein interaction (PPI) network analysis, and the hub-bottleneck nodes of analyzed networks were introduced.

A number of 140, 874, and 1519 significant DEGs were evaluated via PPI network analysis. A biomarker panel including ALB, CTNNB1, COL1A1, POSTN, LUM, and ANXA2 is presented as a biomarker panel to detect PDAC in the early stage. Two biomarker panels are suggested to recognize other stages of illness.

It can be concluded that ALB, CTNNB1, COL1A1, POSTN, LUM, and ANXA2 and also FN1, HSP90AA1, LOX, ANXA5, SERPINE1, and WWP2 beside GAPDH, AKT1, EGF, CASP3 are suitable sets of gene to separate stages of PDAC.

The purpose of the research was to test the fit of the structural modeling of internal and external entrapment on childhood trauma with regard to the mediating role of paranoid thoughts in medical students of Qom province.

This research was a fundamental and descriptive-correlational research study using structural equation modeling. The statistical population of the research was made up of all medical students of Islamic Azad University of Qom province, who were studying in the academic year 2022-2023. The sample size of the study included 271 participants, who were selected based on available sampling method. To collect and measure the data, Bernstein et al.'s (2003) childhood trauma questionnaire, Gilbert and Allan's (1998) entrapment questionnaire, and Green et al.'s (2008) paranoid thoughts questionnaire were directly used. The collected data were analyzed by correlation matrix method and structural equation modeling. SPSS 26 and Smart PLS3 were used for data analysis.

The findings of the research showed that the indicators of the proposed model have an acceptable fit. The results showed that childhood trauma and paranoid thoughts have a direct effect on the entrapment of internal and external at 0.95 % confidence level. Also sobel test results indicated that childhood trauma has an indirect effect on the internal and external entrapment through mediating paranoid thoughts.

Childhood trauma and paranoid thoughts in medical students can directly and indirectly affect the internal and external entrapment.

Positive role of coffee consumption on regulation of human blood sugar has been highlighted by researchers. On the other hand, metformin is the common drug against type 2 diabetes. This study aimed to explore possible ways to use coffee as a complementary agent beside metformin or independently versus type 2 diabetes. Proteomic data about effect of two major compounds of coffee (caffeine and trigonelline) on improvement of diabetic condition was searched and analyzed via protein-protein interaction (PPI) network analysis and gene ontology enrichment. Gene expression profiles of human whole blood of diabetic patients (responsive to metformin) versus control were extracted from GSE83983 which is recorded in Gene Expression Omnibus (GEO) database. After pre-evaluation of data by GEO2R program, the significant differentially expressed genes (DEGs) were assessed via PPI network analysis and regulatory network evaluation. Caffeine and trigonelline effectively regulate the glycolytic processes to fight against diabetic condition. HSP90AA1, TLR4, RELA, ARRB, LRRK2, STAT5B, LYN, and TLR2 genes that are involved in diabetes were affected significantly by metformin. It can be concluded that coffee consumption can improve sugar regulation in diabetes similar to metformin. IT seems that the optimized consumption quantity of coffee can be considered as controller of blood sugar in diabetic patients.

During the COVID-19 pandemic, nurses were praised as the main heroes of the fight against the disease, but pandemic conditions affected the balance between their work and family responsibilities. To this end, the present study aimed to explore the incidence of work-family conflict in nurses during the COVID-19 pandemic. This descriptive cross-sectional study was conducted at COVID-19 referral hospitals in Mazandaran Province, Iran, in 2021. The participants were 200 nurses who were selected using convenience and purposive sampling. The data were collected using the Work-Family Conflict Scale (WAFCS), which was distributed and completed online. The collected data were analyzed with SPSS-16 software using descriptive and inferential statistics. Most of the participants were working as nurses for an average of seven years and had been caring for COVID-19 patients for at least 4-6 months until the time of data collection. The mean work-family conflict score was 78.80±1.1. The data also showed that the severity of work interference with family (WIF) in both time and strain dimensions (83.81±0.3) was significantly higher than the severity of family interference with work (FIW) (65.90±0.3) in these two dimensions.  Nurses faced an imbalance between work and family functions during the COVID-19 pandemic. Since nurses are at the forefront of the fight against the pandemic, it is necessary to pay special attention to their working conditions and family life. Moreover, effective and practical solutions should be provided by managers to resolve work-family challenges.

 The symptoms of coronavirus disease 2019 (COVID-19) range from asymptomatic to severe respiratory distress or death. Reviews on potential COVID-19 treatments show no established therapy. Photobiomodulation can help in reducing inflammation and speed up tissue repair. In addition, due to its few side effects, it appears to be effective in restricting COVID-19. Therefore, it was decided to use this method in disease control to achieve the systemic impact of intravascular photobiomodulation therapy in this study.

 A total of 60 patients were randomly divided into three groups of 20 subjects: A control group that received common treatments for COVID-19, a group treated with a low-power gallium arsenide laser diode (660 nm) with an output dose of 2 J/cm2 for 7 minutes and 5 days in a row in addition to standard treatments, and another group that received common treatments with the same laser dose at the same time as the first group with a low-power diode laser (450 nm). Laboratory data and clinical criteria between groups were compared before and after the treatment.

 An increase in O2 and partial pressure of oxygen (PO2) was significant in the two laser therapy groups (P < 0.05). In addition, the partial pressure of carbon dioxide (PCO2) decreased significantly in the blue laser group (mean difference = -1.44 ± 12.72). The COP score was reduced in all groups; however, only in the blue laser group the reduction in COP score was significant (P < 0.05). In the blue laser group, the COP score before and after the treatment was reduced.

 The use of an intravenous laser with red and blue wavelength with an output dose of 2 J/cm2 for 7 minutes and 5 days in a row, in addition to standard treatments, showed the improvement of oxygenation (O2 and PO2 in arterial blood gas [ABG]) and the reduction of inflammatory factors (erythrocyte sedimentation rate [ESR] and C-reactive protein [CRP]) and COP scores. However, further extensive studies are needed to prove the therapeutic effects of intravenous lasers, along with the usual treatments for COVID-19.

Amyloidosis is a rare disease characterized by the extracellular deposition of a misfolded protein in multiple organs. Cutaneous amyloidosis (CA) is diagnosed by detecting amyloid deposition in the skin. Lichen amyloidosis (LA) and macular amyloidosis (MA) without visceral involvement are two of its more prevalent types. This case-control study was conducted to evaluate C4d staining in amyloidosis to determine whether it could be used as a diagnostic tool for amyloidosis. Moreover, the results of C4d expression in amyloidosis with colloid bodies in lichen planus (LP) were compared. Therefore, 41 cases of CA and 43 cases of LP were selected. All samples were stained with C4d immunostain. 12 of 41 cases of CA had apple green birefringence; however, all of them were positive for C4d, the same as the LP group. The CA group had 100% C4d and 29% Congo red sensitivities (P < 0.05). C4d had 100% sensitivity for colloid bodies in LP. Therefore, the C4d stain could serve as a new IHC marker for highlighting the colloid bodies. C4d immunohistochemical (IHC) staining could be a very valuable ancillary tool for diagnosing amyloidosis, although it did not differentiate amyloid deposition from colloid bodies of LP.

Cisplatin’s common use as an anti-neoplastic drug poses significant challenges due to its adverse effects, including renal disorders, neuropathies, hearing impairment, and gastrointestinal issues.

A comprehensive search was done across major bibliographic databases, including PubMed, Embase, Web of Science, Google Scholar, and Scopus on cisplatin’s application in various cancer treatments. A manual examination of article reference lists was conducted, collecting data from 1990 to October 2023 for up-to-date research analysis.

Cisplatin primarily acts by binding to DNA in the cell nucleus and disrupting DNA transcription and replication, leading to cytotoxicity and malignant cell destruction. Mechanisms of resistance included altered drug absorption, increased efflux and detoxification, modified targets, and increased DNA repair. Interactions with matrix proteins, pH changes, and food affect cisplatin effectiveness. Cisplatin-induced DNA damage mainly forms DNA adducts, causing intra- and inter-strand cross-links. Despite its therapeutic benefits, inevitable adverse effects, like nephrotoxicity, ototoxicity, gastrointestinal diseases, hepatotoxicity, cardiovascular issues, and neuropathy exist. Strategies to mitigate these include hydration therapy, thiol-containing agents, antioxidants, and modulators. Combination therapy enhances cisplatin efficacy.

Cisplatin is a potent anticancer tool marked by challenges from adverse effects and emerging resistance. Ongoing research focuses on combined therapeutic approaches and supports interventions to enhance efficacy and reduce adverse effects, fostering optimism for better cancer treatments.

Okadaic acid (OA) is a toxin of polluted shellfish. Consuming the contaminated shellfish is accompanied by diarrhea and paralytic and amnesic disorders. There is a correlation between diarrhea and the consumed OA. Determining the critical targeted genes by OA was the aim of this study.

The transcriptomic data about the effect of OA on human intestinal caco-2 cells were extracted from gene expression omnibus (GEO) and evaluated via the GEO2R program. The significant differentially expressed genes (DEGs) were included in a protein-protein interaction (PPI) network and the central nodes were enriched via gene ontology to find the crucial affected biological terms.

Among the 178 significant DEGs plus 50 added first neighbors, four hub-bottleneck genes (ALB, FOS, JUN, and MYC) were determined. Twenty-eight critical biological terms were identified as the dysregulated individuals in response to the presence of OA. “ERK1/2-activator protein-1 (AP-1) complex binds KDM6B promoter” was highlighted as the major class of biological terms.

It can be concluded that down-regulation of ALB as a potent central gene leads to impairment of blood homeostasis in the presence of OA. Up-regulation of the other three central genes (JUN, FOS, and MYC) grossly affects the vital pathways in the human body.

Due to weak diagnosis and treatment of PDAC, detection of PDAC possible biomarkers in early stage is the main aim of this study.

Pancreatic ductal adenocarcinoma (PDAC) is known as an exocrine cancer with a 5-year overall survival of 11%.

Gene expression profiles of early stage of PDAC tissue and normal tissue are downloaded from gene expression omnibus (GEO) and evaluated via GEO2R. The significant differentially expressed genes (DEGs) are investigated via protein-protein interaction (PPI) network analysis and gene ontology.

 Among 104 DEGs, ALB, COL1A1, COL1A2, MMP1, POSTN, PLAU, and COL3A1 were pointed out as hub nodes. “Gelatin degradation by MMP1, 2, 3, 7, 8, 9, 12, 13” group of 52 biological terms were identified as the main affected terms.

In conclusion, ALB, MMP1, and COL1A1 genes were highlighted as possible biomarkers of early stage of PDAC. Dysfunction of extracellular matrix was identified as a main event in patients.

Maintaining a healthy balance between commensal, and pathogenic bacteria within the gut microbiota is crucial for ensuring the overall health, and well-being of the host. In fact, by affecting innate, and adaptive immune responses, the gut microbiome plays a key role in maintaining intestinal homeostasis and barrier integrity. Dysbiosis is the loss of beneficial microorganisms and the growth of potentially hazardous microorganisms in a microbial community, which has been linked to numerous diseases. As the primary inducer of circadian rhythm, light can influence the human intestinal microbiome. Photobiomodulation therapy (PBMT), which is the use of red (630-700 nm), and near-infrared light (700 and 1200 nm), can stimulate healing, relieve pain, and reduce inflammation, and affect the circadian rhythm and gut microbiome beneficially. Our focus in this paper is on the effects of PBMT on gut microbiota, to provide an overview of how it can help control gut microbiota dysbiosis-related disorders.

Coffee is a favorable drink in the world with advantages that are documented during different investigations. In the present study, the effect of caffeine which is one of the important compounds of coffee has been evaluated on function of mice liver via network analysis and gene ontology enrichment.

Results of GSE53131 from Gene Expression Omnibus (GEO) were analyzed and the differentially expressed genes (DEGs) were assessed via protein-protein interaction (PPI) network analysis and gene ontology. Cytoscape software and STRING database were used to analyze the data.

Effect of caffeine on mice liver was appeared in the gene expression profiles of the mice liver which were fed with caffeinated and decaffeinated coffee. Acat2, Acly, Acss2, Akr1d1, Ehhadh, Elovl2, Fasn, Fdps, Gsta3, Hmgcr, Ldlr, Lss, Mmab, Mvd, Mvk, Nsdhl, Prodh, Rdh11, and Thrsp that are related mostly to lipid metabolism and cholesterol biosynthesis were pointed out as the discriminator genes in response to caffeine effect on liver function.

In conclusion, assessment of mice liver gene expression profiles revealed that lipid metabolism of the mice liver was affected considerably by consumption of caffeinated coffee versus liver of mice that were fed with decaffeinated coffee. Using caffeine as a preventing factor for hepatic disorders is recommended base on the findings of present study.

 Investigations indicate that Wedelia chinensis extract increases efficacy of prostate cancer treatment. In the present study, the differentially expressed genes (DEGs) of prostate cancer cell line 22RV1 in the presence of W. chinensis extract derived from Gene Expression Omnibus (GEO) were evaluated by gene ontology and pathway analysis. 

 Gene expression profiles of GSE100224 were analyzed by GEO2R. The significant DEGs were assessed via action map analysis. The related biological terms were identified for the significant DEGS. The highlighted dysregulated genes and pathways were discussed. 

 Seventy significant DEGs including 49 up-regulated genes and 21 down-regulated ones were assessed by inhibition, activation, expression, and binding actions. Cytochrome P450 and PTGS2 were highlighted as the crucial DEGs. Estrogen metabolism was pointed as the main targeted pathway.  

 Findings indicated that “estrogen metabolism” and UGT1A1, MAOA, PTSG2, and cytochrome P450 in the 22RV1 cells were the main targeted pathway and genes by W. chinensis .

 Many elder people have knee osteoarthritis (OA). The patients are faced with pain and disability in movement. Given the challenging lifestyle of the patients, finding an efficient therapy approach is necessary. Since low-level laser therapy applies to the treatment of many diseases, it seems it can be a suitable option for the treatment of knee OA. The present study aimed to evaluate the molecular mechanism of laser therapy on knee OA via a protein expression change study.

 The present study examines the gene expression profile of patients with OA in the knee using bioinformatics. The protein expression change profile of synovial fluid of knee OA patients is extracted from the literature and is analyzed based on the rate of expression and interactions between the differentially expressed proteins (DEPs). The results are compared with the DEPs of similar tissue of the treated knee OA patients (from published documents) after laser therapy.

 Apolipoprotein B (APOB) and matrix metallopeptidase 2 (MMP2) were determined as the hub bottlenecks of the protein-protein interaction (PPI) network of synovial fluid of knee OA patients. MMP2, complement 5, transthyretin, and apolipoprotein A-1 from laser-treated patients were related to the PPI network of knee OA patients. The reduction of Interleukin-6 activity was highlighted as a critical event as a function of laser on the human body.

 In conclusion, it was noted that the main phenomenon associated with laser therapy-induced improvement in the condition of knee OA patients is the downregulation of MMP2. 

Introduction:

 Photodynamic therapy (PDT) is a combined method of light and light-activated chemicals that are called photosensitizers (PSs). PDT is recommended as a high cure rate method with fewer side effects and a noninvasive tool to treat cancer. This study aimed to evaluate PDT efficacy as a therapeutic method against actinic keratoses in patients via protein-protein interaction (PPI) network analysis by using the gene expression profiles of Gene Expression Omnibus (GEO).

Twenty-one gene expression profiles were extracted from GEO and analyzed by GEO2R to determine the significant differentially expressed genes (DEGs). The significant DEGs were included in PPI networks via Cytoscape software. The networks were analyzed by the “Network Analyzer”, and the elements of the main connected components were assessed.

There were three main connected components for the compared sets of the gene expression profiles including the lesional region of skin before (Before set) and after (After set) PDT versus healthy (healthy set) skin and before versus after. The before-health comparison showed a partial similarity with the After-Healthy assessment. The before-after evaluation indicated that there were not considerable differences between the gene expression profile of the lesional region before and after PDT.

In conclusion, PDT was unable to return the gene expression pattern of the actinic keratoses skin to a healthy condition completely.

The coronavirus disease (COVID-19) was extended to the entire population in China and around the world, and its mortality rate was about 3.4%. The impact of laser therapy on chronic respiratory diseases has been shown in previous studies. This study was aimed at examining the effects of laser acupuncture (LA) on patients with severe COVID-19.

In the present study, 60 patients with a positive reverse transcription-polymerase chain reaction (RT-PCR) test were assigned to the intervention and control groups (30 patients in each group). The intervention group was treated with LA, that is, laser light with low energy on acupuncture points, once a day for five consecutive days.

The participants’ mean age in the intervention and control groups was 48.96 ± 12.65 and 53.16 ± 12.28 respectively; 70% of the patients were male and 30% of them were female. IL6 had a significant reduction in the intervention group (P value = 0.038) in comparison with the control group (P value = 0.535). Furthermore, the mean admission time in the control group was significantly higher than that in the intervention group (P value = 0.047). However, the mortality rate in the intervention group was zero, but three patients in the control group died.

Our study showed that LA can be used as supportive therapy for routine treatment in patients with severe COVID-19. Moreover, due to LA safety and it’s low cost, it could be recommended as an adjuvant to conventional therapy in patients interested in treating their disease with such a method.

Photodynamic therapy (PDT) is applied as an efficient method for preventing the progress of cancers. Light and a photosensitive compound which is known as photosensitizer (PS) are the main parts of PDT. In the present study, molecular events after using PDT in the presence of a super lethal dose of a PS were assessed via protein-protein interaction (PPI) analysis.

Data were extracted from Gene Expression Omnibus (GEO). The gene expression profiles of the treated human Sk-Cha1 cells via PDT were compared with the control cells. Expressed change analysis and PPI network analysis were administrated via Cytoscape software v 3.7.2 to find the critical differentially expressed genes (DEGs). Regulatory relationships between the central DEGs were evaluated and the highlighted genes were identified.

The significant amounts of gene expression values were grouped and a few DEGs characterized by tremendously expressed values were identified. EGFR, CANX, HSPA5, MYC, JUN, ITGB1, APP, and CDH1 were highlighted as hub-bottleneck DEGs. EGFR, CDH1, and JUN appeared as a set of SEGs, which play a crucial role in response to PDT in the treated Sk-Cha1 cells.

In conclusion, regulatory relationships between EGFR, CDH1, and JUN, which have an effect on the regulation of cellular survival, differentiation, and proliferation, were highlighted in the present investigation.