zeinab raoufi

Vibrio cholerae is an aquatic pathogenic bacterium whose various strains cause diseases such as cholera, acute diarrhea, meningitis and sepsis. Due to the resistance of the strains to different antibiotics, the production of low-risk vaccines that affect all pathogenic strains can be the best method of control. Therefore, in this study, the vaccine potential of VceC outer membrane protein against V. cholerae was evaluated using bioinformatics methods. In addition to determining antigenicity, virulence, and physicochemical properties, B and T cell epitope mapping was performed to design the best peptide subunit vaccine. The results showed that the protein has optimal properties such as presence in all the studied strains, stability and long half-life, good temperature resistance, and solubility during overexpression. Numerous antigenic regions and linear and spatial epitopes were also observed along the protein sequence and structure. According to the location and antigenic properties, two linear and conformational subunit vaccines were designed that can be tested and used against the pathogenicity of different strains of V. cholerae.

Today, the use of natural materials in wound dressing has attracted more attention that can overcome the serious environmental problems caused by conventional synthetic materials. In this study, first gelatin was extracted from Caspian kilka fish waste and then used to make composite films in pure form (G), in combination with xanthan (GX) and Anghouzeh oil (GXA). Mechanical properties, swelling index and surface morphology of the films were investigated. The antibacterial and anti-biofilm effects of G, GX and GXA films were also evaluated. The highest tensile strength was recorded for GX film (37.30±0.59MPa) and the lowest value was recorded for pure gelatin film (11.74±0.2MPa). For elongation at break, the highest value was measured in GXA film (54.87±0.21%) and the lowest was in G film (15.61±0.68%). The highest swelling index was recorded for GX film at 300±15.3%. GXA film presented the highest antimicrobial and anti-biofilm activities. The results of these study showed that the film made of gelatin in combination with xanthan and Anghouzeh oil has ideal mechanical and antibacterial properties to use in wound dressing structure.