فهرست مطالب zohreh kolahi

Inflammatory bowel disease (IBD) is a group of chronic gastrointestinal diseases that pose a challenge to its treatment. This study aimed to investigate the effect of aerobic swimming exercise and in combination with hydrogen-rich water (HRW) on clinical symptoms, histological changes and oxidative stress markers in an animal model of colitis.

Thirty male C57BL6 mice were divided into control, swimming exercise, colitis, colitis+swimming exercise, colitis+swimming exercise+ colitis groups. At the end of study, colon weight and length, spleen weight, disease activity index, histological changes and oxidative stress markers in colon tissue were evaluated. Data were analyzed using one-way or two-way ANOVA and p<0.05 was considered statistically significant.

Results showed that there were no significant differences between control and swimming exercised groups. But, in colitis animals, the disease activity index (such as weight loss, bleeding, and rectal protrusion) in exercised group decreased significantly compared to the colitis group; which showed a further decrease in the combined group (p<0.001). Moreover, colon length was also increased in exercised groups compared to the colitis group (p<0.05). Histological indices (such as leukocyte infiltration) and fibrosis were significantly decreased in exercsied groups compared to the colitis group (p<0.01). Colitis animals had higher malondialdehyde and lower antioxidative markers (superoxihde dismutase, catalase, total thiol) compared to the control group; while exercise and exercise plus HRW significantly improved antioxidative factors.

The results showed that swimming exercise especially in combination with HRW could improve clinical symptoms and histological changes of colon tissue in colitis animals and it can be considered as treatment strategy in patients with colitis.

Ulcerative colitis is one of the major phenotypic forms of inflammatory bowel diseases. The present study aimed to investigate the effect of force swimming exercise on clinical symptoms (disease activity index; DAI), colon histopathology, inflammation and fibrosis, and oxidant/antioxidant balance in dextran sulfate sodium (DSS)‑induced colitis mice.

Male C57BL6 mice were randomly divided into five groups (𝑛 = 6 each): control, exercise, colitis, colitis + sulfasalazine, and colitis + exercise. Exercise was performed by forced swimming six weeks before and during the experiment. Colitis was induced by 1.5% DSS in drinking water. The animals were evaluated for body weight changes and DAI (including changes of body weight, stool consistency, rectal bleeding, and prolapse) during the induction of colitis and treatment. At the end of experiment, colons and spleens were evaluated by H and E and Masson Trichrome stainings. Oxidant (Malon dialdehyde; MDA), and antioxidant markers [total thiol groups, superoxide dismutase (SOD), and catalase activity] were also measured in colon tissue.

Results indicated that exercise in colitis mice significantly improved DAI, colon length, spleen weight, and histological injury score and alleviated fibrotic changes in colon tissue that were comparable to sulfasalazine group. Exercise also restored the oxidant/antioxidant balance in colitis mice by reducing MDA and increasing antioxidative markers including total thiol groups, SOD, and catalase activity.

Taken together, aerobic exercise could improve clinical symptoms and colonic inflammation through, at least, the balancing the oxidative stress markers. Thus, it can be considered in management of colitis patients as effective method.