جستجوی مقالات مرتبط با کلیدواژه « اکسایش » در نشریات گروه « تربیت بدنی »

Type-1 diabetes is associated with the weakness of the body's antioxidant defense and also affects skeletal muscles. This research aimed to investigate the interaction of Berberine supplementation with aerobic exercise on Malondialdehyde (MDA), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPX), and also the Caspase-3 gene expression in quadriceps muscle tissue of type-1 diabetic rats.

35 male Wistar rats were randomly divided into five groups: 1) healthy control, 2) diabetic control, 3) Berberine supplement, 4) aerobic exercise and 5) Aerobic exercise + Berberine supplement. After ensuring the induction of diabetes by STZ in the treatment groups, moderate intensity aerobic exercise was performed for six weeks with a frequency of five sessions per week according to the schedule. Berberine supplementation with a dose of 50 mg/kg was implemented by gavage on all days of the week and half an hour before exercise.

The MDA and SOD levels in Berberine + aerobic exercise group significantly decreased (P<0.0019) and increased (P<0.0063), respectively compared to other groups. The level of GPX in the Berberine + aerobic exercise group had a significant increase compared to the diabetes control and aerobic exercise groups (P<0.0005 and P<0.001, respectively). Also, there was a significant increase in GPX level in the Berberine supplement group compared to the diabetes control group (P<0.0017), however, there was no significant difference between the group of Berberine + aerobic exercise and Berberine supplement (P=0.132). The Caspase-3 gene expression in Berberine + aerobic exercise group had a significant decrease compared to diabetes control and aerobic exercise groups (P<0.0001 and P<0.001, respectively). This variable also had a significant decrease in the Berberine supplement group compared to the diabetes control group (P<0.0005).

It seems that the simultaneous intervention of Berberine supplementation and aerobic exercise has positive synergistic effects and plays an important role in reducing MDA, caspase-3 gene expression, and increasing SOD and GPX in the quadriceps muscle of type-1 diabetic rats.

Cardiovascular disease is main cause mortality in world; that is associated with inflammation and oxidative stress. Lead is a common environmental toxin that long history of known toxicity in impact organs with low antioxidant defense such as heart. Therefore, the purpose of this study was to study effects of exercise training on myocardial inflammation and damage induced by lead acetate in male rats. Twenty-six male rats (Two months of age and Mean body weight of 219±10 g), were randomly divided into three groups include; the Base, lead and aerobic exercise + lead. The rats in the exercise group performed the progressive treadmill running of 15 to 22 m/min for 25 to 64 min, 5 times a week for 8 weeks. Lead and aerobic exercise + lead groups received lead acetate (20 mg/kg) into intra-peritoneal, 3 times a week for 8 weeks. As well as sham group received same amount salin solution. HS-CRP and CPK-MB as index of myocardial inflammation and damage was determined in cardiac tissue and serum by Immunoturbidimetric and ELISA methods, respectively and also MDA and GPx as index of oxidant and antioxidant was measured in serum and heart tissue by Thiobarbituric acid reactive substances and method described by Kalpana methods and then Examination of the data by one- way ANOVA (p<0.05). Results indicated that Injection of lead acetate into intra-peritoneal resulted in significantly increase the CPK-MB and MDA in serum and hs-CRP levels of cardiac tissue and significantly decrease the GPx levels in cardiac tissue (P<0.001). Also, administration aerobic exercise resulted in significantly decrease MDA and CPK-MB levels in serum and nonsignificantly decrease HS-CRP levels (respectively P< 0.01, P<0.001, P<0.086) and significantly increase GPx levels (P<0.001) of cardiac tissue in aerobic exercise + lead group in compared by lead group. Overall, results from the present study significant the role of restrain regular aerobic training on process of inflammation and oxidant and therefore cardiac damage in subjects that exposed to chronic pollution induced by lead acetate.