جستجوی مقالات مرتبط با کلیدواژه « abca1 » در نشریات گروه « تربیت بدنی »

Although the metabolism of blood lipids has been widely considered in studies, lipid metabolism in the brain has attracted more attention in recent years due to its association with some neurological disorders. Recent studies have shown that a number of risk factors for early onset of Alzheimer's are associated with cholesterol metabolism. Therefore, the purpose of this study was to investigate the effect of eight weeks of aerobic training on some factors involved in the metabolism of cholesterol in the hippocampus and its role in improving the cognitive function of male Wistar rats. The subjects of this study were 26 adult male Wistar 8 weeks old (weight 195±20 g). At first, they were randomly divided into two groups: exercise (training on treadmill, 5 days a week for 8 weeks) (13 rats) and control (13 rats). After 8 weeks, the rats in each group were randomly assigned to two other subgroups: (7 rats: behavioral test, 5 rats were sacrificed). The results of one-way ANOVA of Morris water maze showed a significant improvement in learning (P<0.05) and memory (P<0.05) in the exercise group compared to the control group. Also, the results of one-way ANOVA showed a significant increase in the expression of mRNA APOE (P<0.05) and ABCA1 (P<0.05) and no significant difference in the level of Aβ1-42 (P>0.05) in the hippocampus in exercise group compared to the control group. Therefore, aerobic training by a significant increase in the expression of mRNA APOE and ABCA1, which are the main factors of lipid metabolism in the brain and which are involved in the pathology of Alzheimer's disease, can be consistent with improving cognitive function as an effective way of preventing of Alzheimer's disease.

ABCA1, a member of ABC (ATP-binding cassette transporters) family in mammals plays a fundamental role in HDL-C remodeling and thus prevent atherosclerosis. The purpose of this study was to investigate the changes of hepatic ABCA1 and plasma of HDL-C levels of male Wistar rats following four weeks of resistance training. 16 male Wistar rats with the age of 12-14 weeks and a mean body weight of 290± 10g were used. Rats were divided in Two groups of control (n=8) and exercise (n=8). Resistance training consisted of climbing a one meter ladder (26 stairs and 80 degree angle with the ground) with weights tied to the tail of the animal. ELISA kit was used to evaluate the amount of ABCA1 and apoA-I and Plasma HDL level was determined by enzymatic immune method. For data analysis, independent T test was used. The resultes showed that ABCA1 in the liver of rats was higher for about 25 percent higher than the control group, but was not statistically significant. Plasma HDL levels were not significantly different between two groups. Moreover, despite some increases in apoA-I levels they were not statistically significant. Thus, despite the fact that four weeks of resistance training didn’t make statistically significant changes in ABCA1 and HDL and apoA-I, but according to the trend of the data this resistance training protocol seemed to be partly effective Possibly, better results can be achieved by increasing the intensity and leng of the the execises, or by designing a beter combination of exercises.

Aging is associated with changes in blood cholesterol and subsequent cardiovascular disease. The purpose of this study was to investigate the effect of continuous and interval endurance training on micro-RNAs associated with reverse cholesterol transport in Wistar elderly rats. 30 male Wistar rats (23-month-old) With an average weight of 441.75 gram, were randomly divided into two experiment and one control group including continuous training (n = 10), Interval training (n = 10) and the control group (n = 10). Continuous and interval exercise included 8 weeks of training on treadmill and 5 days a week which the continuous group started training with 60% maximum speed for 16 minutes in the first week and continued with 70% maximum speed for 45 minutes from the fourth week to the end of the eighth week. The interval group started exercise at 40-80% of the maximum speed from the first week and continued at 30 to 110% of the maximum speed from the fourth week to the end of the eighth week. After completing training, expression of miR-33 and miR-144 and mRNA expression of ABCA1 were measured RT-PCR. The statistical analysis was performed using ANOVA test with significance level (P <0.05). The expression of miR-33a and miR-144 decreased in both continuous and interval groups compared to control group, but this decrease was only significance for miR-33a (P <0.001). After 8 weeks of training, mRNA expression of ABCA1 gene in the continuous and interval group increased compared to control group. However, this increase was statistically significance only for the interval group (P=0.002). There was no statistically significance difference between the effect of interval and continuous exercises on miR-33a and miR-144 expression. However, there was a significance difference in the mRNA expression of ABCA1 in both continuous and interval groups (P=0.028). Considering the results, it appears that both continuous and interval endurance trainings can decrease miR-33a expression and subsequently increase mRNA expression of the ABCA1, which this increase is higher in interval training compare continuous training.

Back grand: Early obesity and its transfer to the adult, increases likelihood incidence of coronary artery disease (CAD). ATP-binding cassette transporter) ABCA1) as a member of the ABC transporters family plays a crucial role in reverse cholesterol transport and CAD prevention. The purpose of this study was to investigate ABCA1 expression in lymphocytes, plasma apolipoprotein A-I and lipid profiles in response to eight weeks interval aerobic endurance exercise as rope training. Thirty students (17.3±1.1yr, 85.73± 11.68 kg and 28.41± 2.36 kg / m²) volunteered and randomly were assigned into training (n = 15) and control (n =15) groups. Exercise protocol was endurance rope training (8 wk, 4 d/wk and 40 min/d). cell hemolysis and sensitive Elisa method was used for Lymphocyte ABAC1 protein expression.T-student test was employed and data were analyzed by SPSS software (version 16). The independent-samples T-Test results were showed that after 8 weeks interval rope endurance training, the levels of lymphocyte ABCA1 expression(p=0/000) and VO2max(p=0/000) were significantly increased and plasma levels of TG(p=0.017), TC(p=0.001), LDL-c/HDL-c(0.026),TC/HDL-c(p=0.002) and measures of BF%(p=0/015)and BMI(p=0.042) as anthropometric indicators were decreased. Changes of other variables such increasing in Apo A-I, HDL-c, LDL-c and decreasing in body weight, were not significant. Significant level (p>0.05) was considered. The findings of this study proved that 8-week interval aerobic endurance exercise as rope training can be have positive effects on lymphocyte ABCA1 protein expression(as goalkeeper of reverse cholesterol process) and lipid profiles and ultimately in prevention of arteriosclerosis among overweight and obesity adolescents.

Coronary artery disease (CAD) is a leading cause of morbidity and mortality in most countries. This disease is directly associated with increased low–density lipoprotein cholesterol (LDL-c) and is conversely associated with high–density lipoprotein cholesterol (HDL-c) concentrations. ABCA1 is the main exporter of phospholipid and cholesterol from cells to lipid-free or minimum lipid apolipoprotein. Studies clearly indicated that ABCA1 performance has a key role in the process of reverse cholesterol transport (RCT) and an effective role in preventing cardiovascular disease. The purpose of this study was to investigate the effect of two types of single session exercise (aerobic and resistance) on ABCA1 gene expression in lymphocytes in well – trained girls. 24 female athletes from Khorasan province were randomly selected and assigned to three groups: control (n=8), aerobic (AE) (n=8), and resistance (RE) (n=8). Blood samples of all subjects were collected prior to and after exercise session. Lymphocyte was separated using lymphocyte density gradient centrifugation and ABAC1 m–RNA expression was investigated by semi–quantitative RT-PCR. Data were analyzed by one–way ANOVA test (p≤0.05). Results showed that RE and AE group following one session of exercise significantly increased m-RNA expression of lymphocyte ABCA1 gene (f=8.719 and P≤0.001) while this increase in the RE group was higher than AE (P≤0.005 vs. P≤0.01). This study indicated that one session of aerobic and resistance exercise played an effective role in preventing cardiovascular disease though increased ABCA1 gene expression.