جستجوی مقالات مرتبط با کلیدواژه « pgc1α » در نشریات گروه « تربیت بدنی »

The aim of this study was to determine the effect of Quercetin supplement during sedentary period on the signaling pathway of the PGC-1α. Researchers are trying to find out whether Quercetin supplement consumption can be beneficial to preventing atrophy during sedentary period. In this study, 30 male rats (age=2 months old, weight=295±11.80 g, Sprague-Dawley) were selected. They were assigned to 2 groups: control (n=6) and training (n=24). Interval training with the intensity of 16 m started in the first week and reached the speed of 30 m/min. in the eighth week. Then, the rats of the control group and 6 rats of the training group were killed. Then, the rest of training group (n=18) were randomly divided into 3 groups: detraining, sedentation and sedentation-Quercetin supplement. Sedentary groups became inactive for 14 days and Quercetin supplement group was fed with Quercetin (400 mg/day) during this period. After these 14 days, they were killed and the amount of the PGC-1α gene expression of their soleus muscle was measured. The mean weight of the rats was higher than the control and the other two groups (detraining and sedentation) (P=0.001). On the other hand, after 14 days, the sedentation-Quercetin group had the highest whereas the sedentation group had the lowest amount of PGC-1α gene expression (P=0.004). The results of the changes showed that the minimum reduction in the muscle weight and gene expression of the PGC1α during sedentary period can be expected by consuming Quercetin supplement.

Background & The aim of present study was to investigate the effect of eight-week endurance training and diet on the expression of Pgc1α and Ucp1 in adipose tissue of obese and normal C57BL / 6 mice. Accordingly, 48 male C57BL / 6 mice were randomly divided into two groups: high-fat diet group, low-fat diet group (12 weeks).Then, both obese and normal groups divided into four groups based on weight average: High-Fat diet Endurance (HF-E), High-Fat diet sedentary (HF-S), Low-Fat diet Endurance (LF- E), and Low-Fat diet sedentary (LF-S).Groups trained on treadmill for 8 weeks, 5 days a week, with 19 m/min for 45 minutes. 24h after the last training session, the mice were sacrificed. Subcutaneous and brown adipose tissue were isolated and frozen in liquid nitrogen to measure the expression of Pgc1α and Ucp1; using Q-PCR, for gene expression and two-way ANOVA (p˂0/05) as statistical analysis.   The results showed that the high-fat diet caused significant increase in Pgc1α levels in the subcutaneous adipose tissue, while obesity resulted in a significant decrease in it. Additionally, obesity and training resulted in a significant reduction of Pgc1α expression in the brown adipose tissue. Also, the expression of Ucp1 in the obese groups was significantly lower than in the normal groups (p˂0/05). Also it was found that Pgc1α and Ucp1 levels were decreased significantly in the subcutaneous and brown adipose tissues of obese, indicating disorders due to obesity. Training led to increase in the expression of Pgc1α and Ucp1 in the subcutaneous adipose tissue.