جستجوی مقالات مرتبط با کلیدواژه "بند ناف" در نشریات گروه "علوم پایه"

Despite numerous studies on the biological properties and differentiability of umbilical cord-derived mesenchymal stem cells, these studies are still ongoing in order to achieve new findings. Therefore, the present study investigates the biological properties of umbilical cord-derived mesenchymal stem cells and their ability to differentiate into osteocyte and adipocyte.

In this experimental laboratory study, 30 whole placenta specimens were prepared from the mothers under cesarean section and kept under standardized conditions. The mesenchymal cells were isolated by enzymatic method and their morphological characteristics were examined by microscopy and absorption spectroscopy and their biological properties, in particular expression of CD markers, were determined by flow cytometry. Finally, mesenchymal stem cells were cultured in specific media in order to differentiate into osteocyte and adipocyte. Data were analyzed using descriptive statistics.

Morphological and physical examinations by microscope and absorption spectroscopy as well as presenting of CD44, CD73, CD90, and CD105 markers and lacking of CD34 and CD45 markers demonstrated the mesenchymal entity of stem cells. Mesenchymal stem cells successfully differentiated into osteocyte and adipocyte.

Human cord-derived mesenchymal stem cells can differentiate into adult fat and bone cells. In this respect, the use of cord-derived mesenchymal cells could be of significant interest in cell therapy.

Aim: The aim of this study was to evaluate the biocompatibility and differentiation of human umbilical cord mesenchymal stem cells to osteoblast on prepared polycaprolactone scaffolds by electrospinning method, which is located under the surface modification by oxygen plasma.
Material and After isolation of mesenchymal stem cells from human umbilical cord, flow cytometry analysis was performed. Biocompatibility of scaffold was examined using MTT assay. Cells morphology and their adhesion characteristics on the scaffold surface were studied using Scanning Electron Microscopic (SEM) images. Also biodegradability of scaffold calculated using weight loss method. Finally, for study the differentiation of cells on the scaffold surface, osteogenic differentiation medium was used and differentiation was performed by red alizarin staining and RT-PCR.
Results showed that the cells not only had more suitable connection and reproduction abilities on the scaffold, but also were morphologically in a natural condition. Polycaprolacton scaffold is enjoyment of high biocompatibility and its biodegradability up to 15th day done with more rates in comparison with 15 next days. Also red alizarin staining and RT-PCR results showed high rate cell differentiation on polycaprolactone scaffold.
The obtained results of this research suggested that polycaprolactone scaffolds have potential usage as biocompatible biomaterials in tissue engineering and osteogenic differentiation applications.