جستجوی مقالات مرتبط با کلیدواژه « بافت بلاستما » در نشریات گروه « زیست شناسی »

The main goal of this research was to prepare three-dimensional matrix of gingival palate tissues and compare the behavior of bone marrow mesenchymal stem cells and blastema on the scaffolds. The tissues obtained from gingival surgeries in periodontal clinic were decellularized using detergents including sodium dodecyl sulfate and Triton X-100 then after washing and sterilization, were used as scaffold for culturing the rat’s mesenchymal stem cells. The scaffolds were studied by light and electron microscopy before and after 1, 2, and 4 weeks of culture. In addition the prepared scaffolds were assembled within the rings of blastema tissues from rabbit,s pinna. Then the scaffolds were similarly studied by histological techniques after 1, 2, and 4 weeks of culture. Scanning electron microscopy showed that epithelial matrix and collagen fibers in connective tissue remained intact. In both samples epithelium-like structures were observed. Also in the blastema cells migrating to the scaffolds cell secretion was also observed. Human gingival matrix can be a suitable scaffold for studying the cell behaviors. More research should be carried out to determine the identity of differentiated cells, which finally can improve our knowledge regarding the cell-matrix interactions.

The main goal of this research was to prepare a three-dimensional matrix from gingival palate tissues and investigate the possible application of this scaffold in cell culture and tissue engineering. In order to fabricate the scaffolds, the biopsy samples of human palate gingival tissue were preparated surgically and divided in 5 groups then decellulization of the samples were carried out via physical method (put in nitrogen tanks and rinsing with distilled water) as well as chemical method using different concentrations of SDS (0.1%, 0.25%, 0.5%, 0.75% and 1%). Furthermore to evaluate the scaffold prepared with 1% SDS, embryonic like cells from blastema tissue were seeded on the three-dimensional scaffold. Concentration of SDS below 0.5% caused significant reduction (p<0.05) of decellulization of the tissues. Microscopic studies of blastema tissue on the scaffold in different days revealed the penetration, migration, adhesion and differentiation of the cells. This study showed that, it is possible to prepare a natural scaffold frome palatal gingiva tissue using SDS treatment. On the other hand, the results of histologic studies showed that the decellulized scaffolds of palatal gingival might be suitable as three-dimensional bioscaffold for movement, adhesion, differentiation and migration of cells. More investigation is needed to determine the identity of the differentiated cells which further it can help to improve our knowledge about cell-matrix interaction.