جستجوی مقالات مرتبط با کلیدواژه "بلوغ آزمایشگاهی" در نشریات گروه "زیست شناسی"

In vitro maturation (IVM) is a method by which immature oocytes mature in a culture medium and laboratory conditions. Therefore, the culture medium plays a crucial role in the final result of the IVF outcome. This study investigates the impact of six different doses of the Ghrelin hormone agonist GHRP-6 in a synthetic culture medium containing human tubal fluid with serum albumin (HTF+HSA) on the maturation rate of human immature oocytes (GV) within 24 and 48 hours. The oocytes were collected from women under 35 years old, referring to Mehr Infertility Center/ Rasht /Iran, due to non-female factor infertility, and immature oocytes, unsuitable for oocytes cytoplasmic injection, were donated to this project. 30 immature oocytes were used in all 6 experimental and control groups. The criterion of cell maturity and progress of meiosis was the formation of the first polar body after 24 and 48 hours. Fisher's exact test was used to examine statistically significant differences between the groups. The addition of 75 ng/ml of GHRP-6 to the HTF culture medium led to a maturation of 70% of GVs after 24 hours which was statistically significant in comparison with other groups (p < 0.05), but not significant at 48h which was 80% (p > 0.05). There was no significant difference between the 50 ng/ml and 25ng/ml groups and the control group. On the other hand, it was observed that increasing the dose of GHRP-6 in the culture medium led to stop growth and cell death. The results indicated that the Ghrelin hormone agonist with a concentration of 75 ng/ml in the culture medium of human tubule fluid containing human serum can accelerate the meiosis process and better development of oocytes.