جستجوی مقالات مرتبط با کلیدواژه "ترکیبات طبیعی" در نشریات گروه "زیست شناسی"

Marine plants are good source of natural antioxidant compounds. Mangroves are a group of salt-tolerant marine plants of intertidal areas with numerous medicinal properties. In the present study, phytochemical analysis (steroids, alkaloids, phenols, flavonoids and tannins) and antioxidant properties (total phenolic content, DPPH free radical scavenging activity, reduction power and metal chelating activity) of methanolic extract of leaves, stem bark, and roots of mangrove species Avicennia marina was investigated. The mangrove samples were collected from the shores of Gwater Bay in the southeast of Sistan and Baluchestan province. Phytochemical analysis showed that all the tested compounds were present in the extract of mangrove leaves, except alkaloids. While in the stem bark extract, only phenolic compounds, flavonoids and tannins were observed and alkaloids and steroids were not detected. Alkaloids, phenols and flavonoids were discovered in the root extract. The assessment of the antioxidant properties showed that among different parts of mangroves, the highest amount of phenol (6.28 mg gallic acid/ g extract), free radical scavenging (59.18 %) and iron ion reduction power (absorbance at 700 nm: 0.37) was found in the leaves extract. Contrary to these two indicators, the highest amount of iron chelating activity (absorbance at 562 nm: 0.84) was found in mangrove root extract. The results of this research showed that different parts of the mangrove plant, especially its leaves, can be considered as a potential source of natural antioxidants.

Fish mucus layers are the main surface of exchange between fish and the environment, and they possess important biological and ecological functions. In the present study, the antioxidant activity (reducing power, total antioxidant capacity, and free radical scavenging of DPPH) and cytotoxicity (test of saltwater shrimp larvae) of four species of Solea elongate, Euryglossa orientalis, Netuma bilineata, Muraenesox cinereus were investigated. The results showed that E. orientalis fish mucus had the highest reducing power (1.6 ± 0.2), N. bilineata fish mucus had the highest total antioxidant capacity (0.93 ± 0.3) and the highest percentage of free radical scavenging DPPH (89 ± 0.2). In the cytotoxicity test, the highest mortality rate under the influence of S. elongate mucus was about 97.5 ± 2.5. The results of the present study showed that fish mucus can be introduced as a potential source of natural biological compounds with high antioxidant activity and cytotoxicity.

According to researches, sponges produce the most secondary metabolites with biological activities among aquatic animals. In this scientific study, we investigated the antifungal, antibacterial and cytotoxic activities of terpenoid extracted, phytol, from Dysidea avara of Hengam Island, Persia Gulf.

In this study, dried sponge powder was extracted in acetone by maceration method. Then, the terpenoids were extracted by silica gel column chromatography from acetone extract by using n-hexane, ethyl acetate and methanol solvent. Biological activities of extracted compounds were evaluated by   XTT assay on KB / C152, HUT-78 / C185 cancer cell line and Hek293 healthy cell for Cytotoxic, tubular dilution test on Escherichia coli strains, Pseudomonas aeruginosa salubilosa, Klebsiella, Klebsiella Typhi, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Nocardia brasiliensis and the fungal, yeast strains, Aspergillus fumigatus and Candida albicans for antibacterial and antifungal test. The selected phytol was identified by gas chromatography.

The phytol compound was killed fifty percent of cancer cells at concentrations of 67.21μg/ml on oral epithelial cancer cells and concentrations 56.10 μg/ml on lymphocyte cancer cells. Phytol compound were killed gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Bacillus cereus at 200-300 µg/ml concentrations and at 1000 -2000 µg/ml concentrations Gram-negative bacteria Escherichia coli, Pseudomonas salmonella and the fungal Aspergillus, yeast strains C. albicans. This study investigated the effects of phytol fraction extracted from D. avara sponge from the Hengam Island, Persian Gulf. The cytotoxic, antibacterial and antifungal activities of extracted fractions have been demonstrated.

The results showed that complementary experiments, isolation and purification of bioactive compounds can be an effective step in the extraction of natural compounds for the production of drugs, cosmetics and food.

Regarding the negative impacts of using toxic antifouling paints to solve the problem of biofouling, the development of non-toxic paints from natural products and their application in marine industries has gain importance. In the present study, the antifouling properties of Zataria multiflora essential oil in antifouling epoxy paint were investigated. Antibiofouling activity of essential oil was proved by antibacterial, antimicroalgal, antibarnacel and antiartemia activities. The minimum inhibitory concentration was 0.125 and 0.250 mg/L against two bacteria Staphylococcus aureus and Escherichia coli, respectively. According to antimicroalgal adherence assay, essential oil had inhibited the average specific growth rate of Isochrysis galbana with effective concentration of 52.186±17.67mg/L and LC50 was 8.720±1.28 and 35.210±2.37mg/L against antibarnacel and antiartemia, respectively. The essential oil was added to epoxy paint and coated on the fiberglass panel exposed to seawater for three months. At the end of the first and second months, the coated panels containing 3% essential oil had the lowest weight (78.81±22.2 and 178.44±6.58 g, respectively) and lowest fouling cover percentage (63.52±1.92% and 92.05±3.34%, respectively) (P<0.05). Results showed that the effectiveness of antifouling activity of Z. multiflora essential oil depends on the amount of essential oil in the paint and its wash rate in seawater.

Nowadays natural compounds are increasingly used in various fields since they cause fewer adverse side effects than their chemical counterparts. In addition, preparation and production of natural compounds are comparatively simple. The present study aimed to evaluate the anti-cancer effects of brittle star methanol extract on MCF-7 cells. Anti-cancer effects of brittle star methanol extract were examined on the MCF-7 cell line. Moreover, the toxicity of this compound was assessed using cell apoptosis methods, ethidium bromide/acridine orange (EB/AO) staining and Annexin V Apoptosis Detection Kit. According to the MTT assay, the methanolic extract of brittle star could prevent proliferation in a time- and dose-dependent manner. The calculated inhibitory concentration that decreased the cell-viability by half (IC50) was estimated at 150 µg/ml. On the other hand, results of EB/AO staining were indicative of increased apoptotic cells in the treatment group. In addition, results of Annexin V/PI protocol demonstrated a significant enhancement in the percentage of late apoptotic cells (29.02%) in the treatment groups (150 µg/ml) and the percentage of necrotic cells were 2.77% for a period of 24 hours. According to the results, the methanolic extract of Persian Gulf brittle star induced apoptosis in the MCF-7 cell line. These effects could be attributed to the presence of certain active biological compounds, such as Saponins, Naphthoquinones and Carotenoids. Furthermore, our findings suggested that brittle star might have beneficial effects on cancer management. Therefore, this natural marine compound could be used as a complementary element in the prevention and treatment of cancer.

The study of various plants in order to identify natural compounds with medicinal value is of interest to researchers. The aim of this study is to identify the chemical volatile compounds of cedar leaf extract, and to measurement the antibacterial effect of Cedar leaf extracts on Escherichia coli and Staphylococcus aurous and its allopathic effect on seedlings of wheat, barley and sesame.The results showed the existence of 16 different volatile compounds in the extract. Di epi alpha cedrene overall (45.85%) and alpha pinene (12. 52%) were the most volatile compounds in the extract. In Concentrations of 50 and 25 (g/ L) leaf extract of cedar, seeds of three species were able to germinate. In wheat and barley, with increasing concentration, length and weight of shoot and root was significantly reduced. In Sesame with increasing in the concentration of the extract a significant change in the length and weight of shoot and root weren’t observed. The results showed that cedar leaf extract has antibacterial properties. It has more inhibitory effect on Staphylococcus aureus. Percentage inhibition for Staphylococcus aureus and Escherichia coli were 27% and 96%, respectively. Allelopathic potential of different volatile compounds of Cedar leaf extracts can be used to produce of natural and environmentally friendly herbicides. Cedar leaf extract has significant antibacterial properties. Its inhibitory effect could be evaluated against human pathogenic bacteria and could be used as a source of natural antibiotics.