جستجوی مقالات مرتبط با کلیدواژه "دی اتیل هگزیل فتالات" در نشریات گروه "زیست شناسی"

Environmental pollution imposes serious hazards and problems to the living organisms. This study aimed to determine the concentration and seasonal alteration of di-(2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DnBP) in the surface water of Chabahar Bay. Four replicated water samples were taken from the surface layers of seven stations in Chabahar Bay and prepared by dispersive liquid–liquid microextraction (DLLME) method, before being injected into the Gas Chromatography with flame ionization detector. In this method, 0.5 ml of methanol (as diffusion solvent) and 20 µl of carbon tetrachloride (as extraction solvent) were injected quickly into the sea water samples, and after extraction, the deposited phase was taken by a Hamilton syringe and injected into the GC equipment for measurement. Under the optimum extraction and experimental conditions, the method yields a linear calibration curve in the concentration range from 10 to 600 µg/L for target analytes. The enrichment factors for DEHP and DnBP were 775 and 790, the correlation factor 0.997 and 0.997, the limits of detection were 0.04 and 1.78 µg/L and the relative standard deviations of DEHP and DnBP were 0.0036 and 0.058, respectively.The results revealed that the concentration of DEHP and DnBP differ considerably at different stations. Significant differences were also observed between the concentration of DEHP and DnBP at various stations (P ≤ 0.05).

Di(2-ethylhexyl)phthalate (DEHP) is a plasticizer in a variety of consumer products. Given the adverse toxicity effects of DEHP on living organisms, its high consumption and dispersal has been caused a serious concern. . Nowedays, the Fish Embryo Test (FET) method is widely used among the conventional methods of toxicology .The aim of the this study was to determine the acute toxicity of Di(2-ethylhexyl)phthalate on zebrafish (Danio rerio) embryo using FET method to assess both lethal and sublethal endpoints and anomalies effects over 5 days . Zebrafish embryos (3 hours post-fertilization) were exposed to different concentrations of DEHP (0.05, 5, 20, 35, 50 and 100 µg/l) for 120 h, then, lethal and sub-letahl endpoints of embryos evaluated and data were analized using Probit software. The LC50, NOEC and LOEC values obtained for DEHP were 46.14, 12.08 and 22.06 µg/l, respectively. Also, There were abnormalities including coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac, malformation of tail and lack of heartbeat in the embryos. This study showed that DEHP is a teratogenic pollutants and the application of FET method is / an alternative method for developmental toxicity tests to predict effects of emerging pollutants on aquatic organisms.