جستجوی مقالات مرتبط با کلیدواژه « پلاسما » در نشریات گروه « زیست شناسی »

Heavy metals are one of the most important environmental problems and have caused many concerns due to their carcinogenic properties, non-renewability and biological accumulation. Considering the expansion of lands contaminated with heavy metals and the inevitable cultivation of strategic grains such as wheat in these lands and the fact that according to research wheat accumulates more cadmium than other grains, therefore the purpose of this research is to investigate the effect of plasma Atmospheric cold and cadmium were on the activity levels of antioxidant enzymes, proline and the amount of protein and soluble sugars on two cultivars Karim and Gonbad. This experiment was carried out in a factorial form in a completely randomized design with four replications in the greenhouse of Mohaghegh Ardabili University. Factors included wheat cultivars (susceptible cultivar Gombad and resistant cultivar Karim), atmospheric cold plasma at three levels (control, 60 and 120 seconds) and treatment with cadmium metal at four levels (control, 50, 100 and 150 µM). The results showed that the amount of soluble sugar, protein, methionine amino acid concentration, catalase and superoxide dismutase enzymes, and root length were affected by the cultivar × plasma × cadmium interaction. The results of the comparison of three-way effects showed that Gombad wheat cultivar had the highest amount of methionine amino acid in plasma 60 and cadmium 150 with an average of 0.22 (nanmol/g). In general, it can be concluded from the results that the stress caused by the increase of cadmium and plasma concentration on the physiological processes of wheat is different and both cultivars use different mechanisms to deal with cadmium stress. Kareem and Gonbad wheat cultivars spend all their energy in the synthesis of factors involved in the defense mechanism in order to adapt and bear more in order to deal with the development and accumulation of toxic concentrations of this heavy metal in stress conditions. The results of the evaluation of cultivars showed that with the increase in the concentration of cadmium chloride, the amount of catalase, polyphenol oxidase, superoxide dismutase and ascorbate peroxidase decreased and the amount of soluble sugar, total protein, lysine and methionine increased. Also, in Gonbad variety, with the increase in the concentration of cadmium chloride, the amount of total protein, polyphenol oxidase enzyme, superoxide dismutase enzyme and ascorbate-peroxidase decreased, which shows the effects of cadmium toxicity and the production of oxygen free radicals. As a result, it follows oxidative damage and growth reduction, and the amount of soluble sugar of catalase enzyme, the amount of lysine and methionine increased. Also, Karim cultivar is more tolerant to cadmium stress than Gonbad cultivar. Therefore, this research showed that cadmium treatment at 150 micromolar in the presence of cold plasma was able to stimulate the defense mechanisms of the plant and make the plant bear the stress.

Plasma-derived Medicinal Products (PDMPs) are categorized as the biological treatments and used to treat rare, chronic, severe and life-threatening conditions, such as primary immunodeficiency syndrome, bleeding disorders (hemophilia A and B), hemolytic diseases, severe infections, burns and liver diseases, as well as other diseases that caused by absence or lack of proper functioning of certain proteins, and lastly result in recessive patient’s quality of life.The first step in the production of PDMPs, is to collect plasma from eligible and healthy donors. This initial stage is complex and is closely monitored by global authorities with high accuracy and sensitivity to ensure the quality and safety of the finished products, as well as the donor's health.The availability and accessibility of manufactured PDMPs strongly depends on the availability of human plasma as its raw material, meaning that sufficient volume of plasma for fractionation is crucial. Therefore, Adopting a suitable national health policy regarding plasma donation, establishing collection centers, and raising public awareness of the importance of plasma donation will improve nation’s culture for adequate supply of PDMPs. in this paper, we decided to provide a brief and complete oversight of the Iranian plasma industry and highlight the significance of covering the market with toll- manufactured products with Iranian plasma.

Gastric cancer is the sixth most frequent malignancy and the fourth leading cause of cancer death in the world with poor survival rate. Early detection may increase patient’s survival and reduce mortality. miRNAs are small non-coding RNAs which function as negative regulators of genes expression in post-transcriptional level. The aim of this study was to evaluate the expression of miR-34a and miR-375 in the plasma of gastric cancer patients in Guilan province.Methods and materials: In this study, 25 gastric cancer patients and 25 healthy individuals in Guilan province during 2018-2019 were enrolled. The expression of miR-34a and miR-375 was evaluated in plasma of patients with Gastric cancer and healthy individuals by Q-RT-PCR method. The correlation between their expression and patient’s clinicopathological characteristics was also investigated. ROC curve analysis of the miRNAs was performed.

There was no significant difference in the plasma level of miR-34a and miR-375 in gastric cancer patients compared to control. There was no significant difference between the the plasma level of miR-34a and miR-375 and clinicopathological features of patients. Area under the curve (AUC) value of ROC analysis for miR-34a was 0.56±0.08 (P=0.42). Area under the curve (AUC) value of ROC analysis for miR-375 was 0.62±0.08 (P=0.14).

Our results suggested that miR-34a and miR-375 probably don’t be appropriate biomarkers for diagnosis of gastric cancer in Guilan province.

In this study, the effects of changes in environmental salinity on some biochemical parameters and body composition in yellowfin seabream (Acanthopagrus latus) were assayed. Fish adapted to salinity of 20 ppt, then suddenly introduced in experimental salinities including 5, 12 and 34 ppt. Each treatment was done in three replicates. In each replicate, 12 fishes with an average initial weight of 100± 10 grams per 300-liter tanks were placed. alues increased in group 12 and 34 ppt (P<0.05). A higher level of triglyceride and lower levels of high density lipoprotein were recorded in 34 ppt compared to other treatments (P<0.05). The proximate composition of body was affected by salinity as moisture was lower in 34 ppt in compare to other groups (P<0.05). In this study, A. latus survived without mortality in experimental salinities, but changes induced for osmoregulation in 5 ppt requires high energy consumption, which reduces the energy available to the fish.

Tissue engineering and replacement of damaged tissue in medical science is very important and more effective than person-to-person transplantation. Therefore, the production of scaffolds from natural and synthetic polymers with desirable properties to reproduce damaged tissues is increasing. The aim of the present study was to investigate the effect of plasma treatment on contact angle or hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds and cell culture efficiency. In the present experimental research, two types of solvents such as pure chloroform and the choloroform80% and dimethyl formaldehyde20% were used for electrospinning solution. The level of electrospun scaffolds was corrected by plasma technology; then, the African green monkey kidney (VERO) cells were cultured on them. The raw or non-treated electrospun scaffold was compared with that of plasma treated in hydrophilicity and cell culture viewpoints. To compare the hydrophilicity of scaffolds, the contact angle of them was measured. The samples treated with plasma show lower contact angle and consequently higher hydrophilicity. C=O and C-O groups increased in the plasma-treated samples in comparison with those of raw samples. Plasma scaffold level correction improved the adhesion, growth, and proliferation of cells compared to non-treated scaffolds. The contact angle of the plasma-treated samples is significantly reduced. Plasma treatment can increase the hydrophilicity of poly-lactic glycolic acid nanofibrous scaffolds, and cell adhesion and growth on plasma-treated scaffolds is better than cell growth and proliferation on non-treated scaffolds.