جستجوی مقالات مرتبط با کلیدواژه "کلی باسیلوز" در نشریات گروه "زیست شناسی"

Extra-intestinal strains of the pathogenic Escherichia coli APEC are one of the most serious threats to the poultry industry in the world and in Iran, with extensive economic losses due to the high mortality rate in broiler herds. Molecular studies and determination of the frequency of virulence factors of APEC strains provide very useful information for designing and manufacturing vaccines against diseases caused by these strains. The aim of this study was to molecular detection of virulence genes iss and fimC in Escherichia coli Isolates from Poultry with Colibacillosis in Ilam City.

This descriptive cross-sectional study was performed on one hundred and fifty APEC isolates collected from general cases of poultry Colibacillosis in the year 1399. First, isolates were identified using selective and ‎differential culture media and standard biochemical tests. PCR test was used to determine the ‎presence of virulence genes iss and fimC among Escherichia coli isolates using a specific primer.

Results of PCR test on one hundred and fifty APEC isolates obtained from Colibacillosis cases ‎using specific primers to detect the genes encoding fimbriae type 1 fimC and the gene ‎Increased serum survival ‎iss showed that 139 isolates (92.66%) had fimC gene. Also, the results ‎showed that among the total isolates, 126 isolates (84%) have the increased serum survival iss gene.‎

The results of this study indicate the high presence of virulence genes iss and fimC in Escherichia coli isolates isolated from avian Colibacillosis in the study area. Detection of these genes can be used as diagnostic markers of pathogenic Escherichia coli in poultry.

Escherichia coli infections in poultry have a word wide spread. Serotypies O1, O2, O8, O35, O78 Escherichia coli is involved in the development of generalized colibacillosis disease. Genes such as F17, Sfa, pap, afa, , fimH , crl , iuc, bla, yja, chu, are considered as an Escherichia coli acuity factoro. the aim of the present study were Phylogentic of Escherichia coli isolates involved in Colibacillosis and cellucitis in broiler chickens in shahrebabak(Kerman Province) by Multiplex PCR Technique 

From 117 samples of Escherichia coli (83 colibacillosis samples and 34 cellulitis samples) were isolated. These samples were examined and confirmed by biochemical methods.

Colibacillosis isolates were belonged to A (54.27%), B1 (7.22%), B2 (6.03%) and D (32.53%) phylogroups. Where as, the isolates from cellulitis cases were belonged to three main phylogroups; A (55.88%), B1 (5.88%) and D (38.24%).

Statistical analysis showed a specific association between the presence of crl virulence gene and Phylogrups of A and D (P<0.05) in colibacillosis isolates.
The results showed that the isolates from both diseases in broiler chickens could be assigned to various phylogenetic groups (mainly A). Also, the virulence genes profile of cellulitis E.coli is completely different from that of colibacillosis in this region