جستجوی مقالات مرتبط با کلیدواژه « caspase 9 » در نشریات گروه « زیست شناسی »

Caspase 3 gene plays a role in the progression of degeneration and caspase 9 in apoptotic, necrotic, and lysosomal deaths, and after the nerve damage, This study aimed to determine the neuroprotective effects of hydro alcoholic extract and fractions of Agrimonia eupatoria plant on neuron density and the change in the expression of caspase 3 and 9 genes 36 male Wister rats weighing were divided to 6 groups including control, compression, treatment A (compression + hydro alcoholic extract in dose of 75 mg/kg), treatment B (compression + ethyl acetate fraction in dose of 75 mg/kg dose), Treatment C (compression +N butanol fraction in dose of 75 mg/kg) and treatment D (compression + aqueous phase with dose of 75 mg/kg). In the control group, after anesthetizing of the rats, the muscle at the sciatic nerve site was split without nerve damage and in compression group and treatment groups; sciatic nerve of the right leg was subjected to compression for thirty seconds. The first intraperitoneal injection of the extract was performed immediately after compression of the nerve and the second injection was performed 7 days later. After twenty-eight days, the rats were subjected to the perfusion method and samples were taken from the lumbar spinal cord. Slides were stained with toluidine blue, the neuron density was measured using a dissector method and the stereology method and the results were compared by t-test and Anova analysis. Spinal cord samples were taken, Total RNA extracted, and cDNA was synthesized, and then, and the expression changes of caspase 3 and 9 genes in the samples were investigated. The results showed that neuronal density increased significantly in all treatment groups compared to the compression group (p < 0.001) and it is more in the n-butanol group than the other groups. The gene expression of Caspase 3 and 9 decreased in all treatment groups compared with compression group (p < 0.01). The extract of this plant can be effective in repairing the nervous system due to the presence of compounds such as tocopherol, which has anti-oxidant and anti-apoptotic effects.

SH-SY5Y is a neuroblastoma cell line which used as a cancer and neurodegenerative disorders model and its neuro-experimental studies. The different diseases cause by a defect in apoptosis pathway. Disruption of apoptotic proteins has an effect on the treatment process and response to drugs. In nerve cells, due to the high expression of apoptosis inhibitory proteins, the efficacy of drugs is low. Combination therapy is one of the developing treatment methods. The aim of this research is to evaluate the effectiveness of doxorubicin drug on apoptosis in SH-SY5Y cells under the conditions of high expression of caspase9. Caspase9 is a key enzyme in intrinsic apoptosis. First, cell viability was obtained through MTT assay under the different drug concentrations. Then, caspase9 gene was transfected in cells and affected by the concentration lower than IC50 of drug, and cell energy level and cell death were checked by different methods. ATP assay showed that the expression of caspase9 with drug lead to ATP decreases. Caspase3/7 activity indicated an increase in cell death by drug and caspase. Propidium staining to hoechst showed that the expression of caspase9 in combination with doxorubicin induce more death. To ensure the expression levels of protein that induces cell death, the amount of caspase3 protein was checked by western blotting, which showed a significant increase in combination of caspase9 and drug. Our findings showed that the induction of caspase9 expression intensifies the effect of drug and the combined treatment may be effective on the responsiveness of neuronal diseases.

Diabetes is a chronic disease that affects the metabolism of sugar or blood glucose. This disease is caused by insulin resistance in the body. Diabetes can lead to various complications in the body, including liver damage. Berberine reduces the amount of damage caused by oxidative stress on mitochondria and increases the antioxidant capacity of liver tissue. exercise training causes mitochondrial biogenesis in diabetic patients. Therefore, the aim of this study was to investigate the effect of aerobic exercise with different doses of berberine chloride on the apoptosis of liver cells in diabetic male rats with streptozotocin.

In this experimental study, 64 male Wistar rats were randomly divided into 8 groups of 8. The groups include the control group, diabetic, diabetic and taking berberine chloride with a dose of 15 mg/kg, diabetic and taking berberine chloride with a dose of 30 mg/kg, diabetic with aerobic exercise, taking berberine chloride with a dose of 15 mg/kg, diabetic with aerobic exercise and the consumption of berberine chloride with a dose of 30 mg/kg, diabetics with aerobic exercise and the control group with aerobic exercise were divided. Intraperitoneal injection of streptozotocin 60mg/kg was used to make diabetic groups diabetic. Berberine chloride (15 and 30 mg/Kg) was taken orally by gavage once a day. The exercise groups performed aerobic exercise for 6 weeks with an intensity of 50-55% of maximum oxygen consumption. 48 hours after the last training session, the liver tissue was extracted to check the changes of caspase3 protein. Using the TUNEL method, the amount of apoptosis in the liver tissue was evaluated. Data were analyzed using one-way analysis of variance and Tukey's follow-up test.

In the present study, diabetic rats showed a significant increase in liver tissue apoptosis and caspase3 protein expression. Treatment of diabetic animals with berberine in doses of 15 and 30 mg/kg led to a significant reduction of apoptotic cells in diabetic rats. The expression of caspase3 protein in the diabetic group with aerobic exercise and berberine chloride consumption at a dose of 30 mg/kg was significantly decreased compared to the diabetic group and berberine chloride consumption at a dose of 15 mg/kg.

It seems that the treatment of diabetic rats with berberine chloride together with exercise activity was able to prevent the occurrence of apoptosis in the liver tissue and improve the liver damage caused by diabetes.

Programmed cell death is a vital cellular process that is highly conserved in evolution. Apoptosis, as a common mode of programmed cell death, is disturbed in the most human malignancies and leads the resistance of cancers to current treatment strategies. Caspase 9 is a key protein in mitochondrial apoptosis. Activated Caspase 9 leads to activation of Caspase 3/7, initiating a caspase cascade and killing cell. In this study, Caspase 9 gene was cloned into pcDNA3.1(+) and its expression and function evaluated in cell.

PCR amplification of Caspase 9 was performed by specific primers and ligated into pcDNA3.1(+) after double-digestion with KpnI and BamHI. After sequencing, pcDNA/Caspase 9 was transfected into SH-SY5Y cells and treated with doxorubicin. Caspase 9 function was determined by its effect on cell death level by trypan blue and PI staining, and Caspase 3 activity, and its expression in cells measured by western blotting.

Caspase 9 gene cloning was done and its expression in cell defined by western blot. Overexpression of Caspase 9 led to autoprocessing following homodimerization and induction of cell death and also increased cell sensitivity to doxorubicin treatment and declined cell viability.

The cloned Caspase 9 was functional in cell and enhanced apoptosis in the treated cells by doxorubicin through self-activation and subsequently amplification of Caspase 3 activation.

In this study the effects of paclitaxel and oxaliplatin on colon cancer cell line (HT29) and expression of apoptotic genes caspase 3, 9, and p53 were examined. Using the MTT method, the cytotoxicity of paclitaxel and oxaliplatin and synergic effect at different concentrations on the HT29 cell line was assessed. The IC50 of paclitaxel and oxaliplatin was determined.The expression level of caspase 3 and 9 was assessed using the Real-Time PCR method after the cells had been exposed to the IC50 concentration. Apoptosis of the cells was done using Annexin V/PI and DAPI staining. The treatment of cells revealed that paclitaxel and oxaliplatin at concentrations of 3.25 and 0.00062 µg/ml , respectively, have the most cytotoxic effects and its IC50 value was determined to be 12.5 µg/ml and 0.00016 and treatment groups at concentrations of 3.25 and 0.00062 µg/ml , respectively, decreased the cell viability and its IC50 value was determined to be 12.5 µg/ml and0.00016µg/m. The expression level of caspase 3 and 9 P53 was also increased in the treated colon cancer cell line. DAPI staining showed apoptosis in the simultaneous treatment groups with. Using Annexin V/PI reveals that 98 percent of the cells in the control group are healthy and a considerable number of the cells in the treated groups have undergone apoptosis. Is. It is clear that paclitaxel and oxaliplatin are effective options for treating colon cancer given their cytotoxicity and stimulation of the apoptotic process in colon cancer cell lines.

The studies indicated that sex steroid hormones affect the digestive system function and proliferation of the cancer cells in the cell and molecular level. This study examines the anti-proliferative effects of Nandrolone on colon cancer (HCT) cell line and evaluates the activity of caspases of 3, 8, and 9. In this experimental study, HCT cells were divided into the control group (without treatment) and groups treated with 0.625, 1.25, 2.5, 5, and 10 mg/ml of Nandrolone. The cytotoxic effect of hormone was measured using MTT. The activity level of caspases 3, 8, and 9 were evaluated by the standard kit. The data were compared among the groups using one-way analysis of variance and t-test. HCT cell viability significantly decreased in the groups treated with 2.5, 5, and 10 mg/ml of Nandrolone compared to the control group (p < 0.001); However, caspase 3 and 9 (p < 0.001) and caspase 8 activity (p < 0.01) significantly increased. This study's findings showed that Nandrolone has a cytotoxic effect on colon cancer cells, and this effect is partly mediated by increasing the caspases of 3, 8, and 9 activities.

Pulmonary hypertension syndrome with high pulmonary arterial pressure, right ventricular hypertrophy and dilation is a problem of broilers. On the other hand it is proved that apoptosis in heart failure and pulmonary hypertension increases. In this study, for the first the effect of vitamin c on apoptosis by measuring the expression of caspase (1) in the heart and lungs of broilers with pulmonary hypertension syndrome was evaluated. T3 as a thyroid hormone was added to the ration after week 1 of rearing. Pulmonary hypertension was induced at 49 days based on RV/TV ratio index. After PCR for caspase1 and b-actin (Housekeeping) genes the density of each band were measured and were recorded as the ratio caspase1 / β-actin and this ratio were compared at different ages in witness groups (the right ventricle and lung). The amount of mRNA of the caspase 1 gene in the right ventricle at 21 and 49 days and in lung tissue at 49 days significantly reduced in the treatment group compared to the control group(p

Breast cancer is the most common cancer and the second leading cause of cancer death in women after lung cancer. Many efforts, including surgery, radiation and chemotherapy, have been made to treat breast cancer. Due to some drawbacks of chemotherapy such as toxicity and drug resistance, some other strategies, including use of marine resources, have recently been developed. Cyanobacteria have some effective compounds that can induce the process of cell death in cancer cells and might be promising candidate for cancer therapy. In the present study, the effect of hydro-alcoholic extract of Oscillatoria cyanobacterium on the viability and gene expression of caspase 3 (CASP3) in MCF-7 breast cancer cell line was examined. Breast cancer cell lines were treated with concentrations of 0.075, 0.15, 0.3, 0.6, 1.2, 2.5, 5 and 10 mg/mL of Oscillatoria extract. The effect of extracts on the viability of cells was evaluated by MTT assay and alteration of caspase 3 gene expression was checked out by Real Time PCR. The results showed that the MCF7 cell viability was reduced in the presence of increasing concentration of the extract with significant differences compared to the control samples. The results of Real Time PCR showed that the expression of caspase 3 significantly increased after 24 h in comparison with the control group.