جستجوی مقالات مرتبط با کلیدواژه « micro propagation » در نشریات گروه « زیست شناسی »

Lythrum salicaria L., commonly referred to as Purple Loosestrife, is a medicinal plant that has been valued for its therapeutic properties for centuries. The aim of this study is to determine the effect of using activated charcoal on establishing the in vitro propagation of L. Salicaria L. In this study, shoot explants (1.5 cm) were excised from buds that were 30 days old with a sterile scalpel blade and then cultured on full‐strength MS medium supplemented with different concentrations of activated charcoal to multiply and increase the length of shoots. Additionally, to investigate the in vitro rooting response, shoot tip explants were cut from elongated shoots and cultured on an MS medium containing different concentrations of NAA with or without activated charcoal with three replicates in each experiment. MS medium with 0.5 g/L activated charcoal had the highest mean shoot length (7.1 cm ± 0.15) and mean number of shoots per explant (2.4±0.11). The results show that 0.5 mg/L NAA and 0.2 g/L activated charcoal provide the best response for rooting. The improved protocol can be utilized to grow roots in micro shoots of L. salicaria, which is an important stage in the micro propagation of L. salicaria.

Hypericum perforatum L. (St. Johns’ wort) is the most commercially important species of the genus Hypericum and contains a wide range of components including naphthodianthrones, phloroglucinols, tannins, xanthones, phenolic acids and essential oil. In order to establish an efficient protocol for regeneration, the effects of explant type and plant growth regulators on direct and indirect shoot regeneration in H. perforatum were evaluated. According to obtained results the media supplemented with 0.1 mg l-1 Benzyl Adenine (BA) was effective for shoot proliferation from shoot tip explants of H. perforatum that showed the highest shoots number (15.5 shoots per explant) and shoot height (2.07 cm). In second experiment a method for rapid micro propagation of H. perforatum through indirect plant regeneration from calli has been developed. The results demonstrated that a combination of auxin and cytokinin was needed for optimum callus induction and leaf segments were suitable explant for callus induction in H. perforatum. Callus induction was observed in most studied treatments but the highest callus volume (1.43 cm3) was obtained by leaf segments in media supplemented with 0.25 mg l-1 2,4Dichlorophenoxyacetic acid (2,4-D) mg l-1 Kinetin. Successful shoot regeneration from callus was observed in MS medium containing 0.5 mg l-1 BA, which resulted the highest shoot proliferation rate (61.75%) and maximum number of induced shoots (9 shoots per explant). All of shoots formed root in media with 0.5 mg l-1 Indole-3-Butyric Acid (IBA) on which 100% of the regenerated shoots developed roots with an average number of 5 roots per shoot. The plantlets were acclimatized and transferred to the greenhouse with 80% survival. This in vitro propagation protocol should be useful for conservation as well as mass propagation of H. perforatum plant.

Ficus religiosa has great mythological, religious, and medicinal importance in the culture of India and Nepal since times immemorial. The present paper was done to evaluate the potential of sodium hypochlorite in controlling the contamination of F. religiosa micro-propagation and seed germination of F. religiosa. In this study, a factorial experiment based on a completely randomized design with 18 treatments including six different sodium hypochlorite concentrations (0, 5, 10, 15, 20, and 25 %) and three soaking time of explants (5, 10 and, 15 min) with three replications was conducted. The seeds were inoculated on one-tenth strength of MS (Murashige and Skoog, 1962) medium. The lowest rate of contamination (0%) was obtained in treatments containing 20% Sodium hypochlorite at 10 and 15 min immersion and 25% Sodium hypochlorite at 5, 10 and 15 min immersion. The highest seed germination (63.33%) was observed in treatments including 10% Sodium hypochlorite at 5 and 10 min immersion.